Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0884 and EX28DRAFT_0885 are separated by 66 nucleotides EX28DRAFT_0885 and EX28DRAFT_0886 are separated by 31 nucleotides EX28DRAFT_0886 and EX28DRAFT_0887 overlap by 41 nucleotides EX28DRAFT_0887 and EX28DRAFT_0888 are separated by 200 nucleotides EX28DRAFT_0888 and EX28DRAFT_0889 are separated by 412 nucleotides
EX28DRAFT_0884: EX28DRAFT_0884 - hypothetical protein, at 892,838 to 893,338
_0884
EX28DRAFT_0885: EX28DRAFT_0885 - Protein of unknown function (DUF2732), at 893,405 to 893,614
_0885
EX28DRAFT_0886: EX28DRAFT_0886 - hypothetical protein, at 893,646 to 893,909
_0886
EX28DRAFT_0887: EX28DRAFT_0887 - Bacteriophage replication gene A protein (GPA), at 893,869 to 896,226
_0887
EX28DRAFT_0888: EX28DRAFT_0888 - hypothetical protein, at 896,427 to 896,684
_0888
EX28DRAFT_0889: EX28DRAFT_0889 - hypothetical protein, at 897,097 to 898,164
_0889
Position (kb) 893
894
895
896
897 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 892.904 kb on + strand, within EX28DRAFT_0884 at 892.905 kb on - strand, within EX28DRAFT_0884 at 892.996 kb on - strand, within EX28DRAFT_0884 at 893.197 kb on + strand, within EX28DRAFT_0884 at 893.198 kb on - strand, within EX28DRAFT_0884 at 893.296 kb on + strand at 893.297 kb on - strand at 893.305 kb on - strand at 893.327 kb on + strand at 893.332 kb on - strand at 893.336 kb on + strand at 893.399 kb on - strand at 893.527 kb on + strand, within EX28DRAFT_0885 at 893.569 kb on - strand, within EX28DRAFT_0885 at 893.569 kb on - strand, within EX28DRAFT_0885 at 893.572 kb on - strand, within EX28DRAFT_0885 at 893.572 kb on - strand, within EX28DRAFT_0885 at 893.572 kb on - strand, within EX28DRAFT_0885 at 893.653 kb on + strand at 893.666 kb on + strand at 893.676 kb on + strand, within EX28DRAFT_0886 at 893.677 kb on - strand, within EX28DRAFT_0886 at 893.798 kb on + strand, within EX28DRAFT_0886 at 893.798 kb on + strand, within EX28DRAFT_0886 at 893.798 kb on + strand, within EX28DRAFT_0886 at 893.798 kb on + strand, within EX28DRAFT_0886 at 893.798 kb on + strand, within EX28DRAFT_0886 at 893.799 kb on - strand, within EX28DRAFT_0886 at 893.904 kb on - strand at 893.907 kb on + strand at 893.908 kb on - strand at 893.910 kb on + strand at 893.996 kb on - strand at 893.996 kb on - strand at 894.124 kb on - strand, within EX28DRAFT_0887 at 894.124 kb on - strand, within EX28DRAFT_0887 at 894.124 kb on - strand, within EX28DRAFT_0887 at 894.124 kb on - strand, within EX28DRAFT_0887 at 894.133 kb on + strand, within EX28DRAFT_0887 at 894.135 kb on + strand, within EX28DRAFT_0887 at 894.135 kb on + strand, within EX28DRAFT_0887 at 894.136 kb on - strand, within EX28DRAFT_0887 at 894.164 kb on + strand, within EX28DRAFT_0887 at 894.164 kb on + strand, within EX28DRAFT_0887 at 894.176 kb on - strand, within EX28DRAFT_0887 at 894.289 kb on + strand, within EX28DRAFT_0887 at 894.289 kb on + strand, within EX28DRAFT_0887 at 894.290 kb on - strand, within EX28DRAFT_0887 at 894.306 kb on + strand, within EX28DRAFT_0887 at 894.307 kb on - strand, within EX28DRAFT_0887 at 894.345 kb on - strand, within EX28DRAFT_0887 at 894.367 kb on + strand, within EX28DRAFT_0887 at 894.378 kb on - strand, within EX28DRAFT_0887 at 894.378 kb on - strand, within EX28DRAFT_0887 at 894.447 kb on - strand, within EX28DRAFT_0887 at 894.448 kb on + strand, within EX28DRAFT_0887 at 894.645 kb on - strand, within EX28DRAFT_0887 at 894.711 kb on + strand, within EX28DRAFT_0887 at 894.765 kb on + strand, within EX28DRAFT_0887 at 894.766 kb on - strand, within EX28DRAFT_0887 at 894.795 kb on + strand, within EX28DRAFT_0887 at 894.795 kb on + strand, within EX28DRAFT_0887 at 894.813 kb on - strand, within EX28DRAFT_0887 at 894.813 kb on - strand, within EX28DRAFT_0887 at 894.837 kb on + strand, within EX28DRAFT_0887 at 894.888 kb on + strand, within EX28DRAFT_0887 at 894.889 kb on - strand, within EX28DRAFT_0887 at 895.013 kb on - strand, within EX28DRAFT_0887 at 895.016 kb on + strand, within EX28DRAFT_0887 at 895.016 kb on + strand, within EX28DRAFT_0887 at 895.017 kb on - strand, within EX28DRAFT_0887 at 895.042 kb on - strand, within EX28DRAFT_0887 at 895.065 kb on - strand, within EX28DRAFT_0887 at 895.170 kb on + strand, within EX28DRAFT_0887 at 895.245 kb on + strand, within EX28DRAFT_0887 at 895.267 kb on + strand, within EX28DRAFT_0887 at 895.269 kb on + strand, within EX28DRAFT_0887 at 895.270 kb on - strand, within EX28DRAFT_0887 at 895.279 kb on + strand, within EX28DRAFT_0887 at 895.280 kb on - strand, within EX28DRAFT_0887 at 895.280 kb on - strand, within EX28DRAFT_0887 at 895.280 kb on - strand, within EX28DRAFT_0887 at 895.392 kb on + strand, within EX28DRAFT_0887 at 895.540 kb on + strand, within EX28DRAFT_0887 at 895.660 kb on + strand, within EX28DRAFT_0887 at 895.660 kb on + strand, within EX28DRAFT_0887 at 895.791 kb on + strand, within EX28DRAFT_0887 at 895.827 kb on + strand, within EX28DRAFT_0887 at 895.935 kb on - strand, within EX28DRAFT_0887 at 896.167 kb on - strand at 896.199 kb on + strand at 896.224 kb on + strand at 896.224 kb on + strand at 896.225 kb on - strand at 896.228 kb on + strand at 896.229 kb on - strand at 896.229 kb on - strand at 896.249 kb on + strand at 896.249 kb on + strand at 896.379 kb on - strand at 896.455 kb on + strand, within EX28DRAFT_0888 at 896.455 kb on + strand, within EX28DRAFT_0888 at 896.476 kb on + strand, within EX28DRAFT_0888 at 896.580 kb on + strand, within EX28DRAFT_0888 at 896.580 kb on + strand, within EX28DRAFT_0888 at 896.788 kb on + strand at 896.788 kb on + strand at 896.845 kb on + strand at 896.848 kb on - strand at 896.848 kb on - strand at 896.859 kb on - strand at 896.900 kb on - strand at 896.908 kb on - strand at 896.920 kb on - strand at 896.939 kb on - strand at 896.944 kb on + strand at 896.975 kb on - strand at 897.005 kb on - strand at 897.012 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 892,904 + EX28DRAFT_0884 0.13 +0.0 892,905 - EX28DRAFT_0884 0.13 +0.3 892,996 - EX28DRAFT_0884 0.32 +1.8 893,197 + EX28DRAFT_0884 0.72 -0.4 893,198 - EX28DRAFT_0884 0.72 -0.3 893,296 + -1.7 893,297 - -0.4 893,305 - -3.0 893,327 + +0.5 893,332 - +0.0 893,336 + -0.2 893,399 - +0.0 893,527 + EX28DRAFT_0885 0.58 +0.6 893,569 - EX28DRAFT_0885 0.78 -0.5 893,569 - EX28DRAFT_0885 0.78 -0.1 893,572 - EX28DRAFT_0885 0.80 -0.4 893,572 - EX28DRAFT_0885 0.80 +0.2 893,572 - EX28DRAFT_0885 0.80 +0.9 893,653 + -0.8 893,666 + +1.8 893,676 + EX28DRAFT_0886 0.11 +0.2 893,677 - EX28DRAFT_0886 0.12 -0.6 893,798 + EX28DRAFT_0886 0.58 -1.6 893,798 + EX28DRAFT_0886 0.58 -0.2 893,798 + EX28DRAFT_0886 0.58 +0.3 893,798 + EX28DRAFT_0886 0.58 +0.4 893,798 + EX28DRAFT_0886 0.58 -0.6 893,799 - EX28DRAFT_0886 0.58 -1.4 893,904 - -0.4 893,907 + +2.0 893,908 - +2.2 893,910 + -0.3 893,996 - -0.7 893,996 - +0.4 894,124 - EX28DRAFT_0887 0.11 -1.0 894,124 - EX28DRAFT_0887 0.11 +0.1 894,124 - EX28DRAFT_0887 0.11 -0.1 894,124 - EX28DRAFT_0887 0.11 -0.9 894,133 + EX28DRAFT_0887 0.11 -0.1 894,135 + EX28DRAFT_0887 0.11 -0.5 894,135 + EX28DRAFT_0887 0.11 +0.0 894,136 - EX28DRAFT_0887 0.11 -3.2 894,164 + EX28DRAFT_0887 0.13 +0.4 894,164 + EX28DRAFT_0887 0.13 -0.2 894,176 - EX28DRAFT_0887 0.13 +1.4 894,289 + EX28DRAFT_0887 0.18 -1.1 894,289 + EX28DRAFT_0887 0.18 -0.2 894,290 - EX28DRAFT_0887 0.18 +0.1 894,306 + EX28DRAFT_0887 0.19 +0.2 894,307 - EX28DRAFT_0887 0.19 -0.5 894,345 - EX28DRAFT_0887 0.20 +0.3 894,367 + EX28DRAFT_0887 0.21 -1.2 894,378 - EX28DRAFT_0887 0.22 -0.7 894,378 - EX28DRAFT_0887 0.22 +0.3 894,447 - EX28DRAFT_0887 0.25 -1.1 894,448 + EX28DRAFT_0887 0.25 -0.2 894,645 - EX28DRAFT_0887 0.33 -0.7 894,711 + EX28DRAFT_0887 0.36 -3.4 894,765 + EX28DRAFT_0887 0.38 +0.2 894,766 - EX28DRAFT_0887 0.38 +0.3 894,795 + EX28DRAFT_0887 0.39 -1.0 894,795 + EX28DRAFT_0887 0.39 -0.4 894,813 - EX28DRAFT_0887 0.40 +0.6 894,813 - EX28DRAFT_0887 0.40 -2.0 894,837 + EX28DRAFT_0887 0.41 -1.6 894,888 + EX28DRAFT_0887 0.43 -0.9 894,889 - EX28DRAFT_0887 0.43 +0.9 895,013 - EX28DRAFT_0887 0.49 +0.1 895,016 + EX28DRAFT_0887 0.49 -0.8 895,016 + EX28DRAFT_0887 0.49 +2.8 895,017 - EX28DRAFT_0887 0.49 +0.6 895,042 - EX28DRAFT_0887 0.50 +0.4 895,065 - EX28DRAFT_0887 0.51 -1.5 895,170 + EX28DRAFT_0887 0.55 +0.7 895,245 + EX28DRAFT_0887 0.58 -1.0 895,267 + EX28DRAFT_0887 0.59 -0.2 895,269 + EX28DRAFT_0887 0.59 +0.3 895,270 - EX28DRAFT_0887 0.59 -0.2 895,279 + EX28DRAFT_0887 0.60 +2.5 895,280 - EX28DRAFT_0887 0.60 -3.0 895,280 - EX28DRAFT_0887 0.60 -0.6 895,280 - EX28DRAFT_0887 0.60 -0.7 895,392 + EX28DRAFT_0887 0.65 +0.2 895,540 + EX28DRAFT_0887 0.71 +0.5 895,660 + EX28DRAFT_0887 0.76 +0.9 895,660 + EX28DRAFT_0887 0.76 -0.1 895,791 + EX28DRAFT_0887 0.82 -0.3 895,827 + EX28DRAFT_0887 0.83 -0.4 895,935 - EX28DRAFT_0887 0.88 +0.3 896,167 - +0.7 896,199 + +0.1 896,224 + -0.3 896,224 + -0.6 896,225 - -0.4 896,228 + -0.0 896,229 - -0.7 896,229 - +1.2 896,249 + -0.8 896,249 + -0.1 896,379 - +0.3 896,455 + EX28DRAFT_0888 0.11 -0.2 896,455 + EX28DRAFT_0888 0.11 +1.5 896,476 + EX28DRAFT_0888 0.19 +0.4 896,580 + EX28DRAFT_0888 0.59 +0.0 896,580 + EX28DRAFT_0888 0.59 +0.3 896,788 + +1.4 896,788 + -0.2 896,845 + -3.0 896,848 - -2.6 896,848 - -2.0 896,859 - -3.2 896,900 - -0.1 896,908 - +0.5 896,920 - -0.0 896,939 - -0.4 896,944 + -3.0 896,975 - +0.5 897,005 - +0.8 897,012 + +1.4
Or see this region's nucleotide sequence