Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0730 and EX28DRAFT_0731 are separated by 161 nucleotides EX28DRAFT_0731 and EX28DRAFT_0732 are separated by 74 nucleotides EX28DRAFT_0732 and EX28DRAFT_0733 are separated by 23 nucleotides EX28DRAFT_0733 and EX28DRAFT_0734 are separated by 506 nucleotides EX28DRAFT_0734 and EX28DRAFT_0735 overlap by 286 nucleotides
EX28DRAFT_0730: EX28DRAFT_0730 - YobH-like protein, at 729,760 to 729,999
_0730
EX28DRAFT_0731: EX28DRAFT_0731 - MgrB protein, at 730,161 to 730,304
_0731
EX28DRAFT_0732: EX28DRAFT_0732 - YebO-like protein, at 730,379 to 730,669
_0732
EX28DRAFT_0733: EX28DRAFT_0733 - Predicted Zn-dependent hydrolases of the beta-lactamase fold, at 730,693 to 731,682
_0733
EX28DRAFT_0734: EX28DRAFT_0734 - RNA, at 732,189 to 732,651
_0734
EX28DRAFT_0735: EX28DRAFT_0735 - Cold shock proteins, at 732,366 to 732,575
_0735
Position (kb) 730
731
732 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 729.704 kb on + strand at 729.872 kb on - strand, within EX28DRAFT_0730 at 729.875 kb on + strand, within EX28DRAFT_0730 at 729.975 kb on + strand, within EX28DRAFT_0730 at 730.022 kb on + strand at 730.034 kb on - strand at 730.034 kb on - strand at 730.100 kb on - strand at 730.133 kb on + strand at 730.305 kb on + strand at 730.305 kb on + strand at 730.625 kb on - strand, within EX28DRAFT_0732 at 730.625 kb on - strand, within EX28DRAFT_0732 at 730.960 kb on - strand, within EX28DRAFT_0733 at 730.968 kb on + strand, within EX28DRAFT_0733 at 731.238 kb on + strand, within EX28DRAFT_0733 at 731.238 kb on + strand, within EX28DRAFT_0733 at 731.267 kb on + strand, within EX28DRAFT_0733 at 731.381 kb on - strand, within EX28DRAFT_0733 at 731.422 kb on - strand, within EX28DRAFT_0733 at 731.444 kb on + strand, within EX28DRAFT_0733 at 731.453 kb on - strand, within EX28DRAFT_0733 at 731.484 kb on + strand, within EX28DRAFT_0733 at 731.668 kb on + strand at 731.697 kb on + strand at 731.704 kb on + strand at 731.706 kb on + strand at 731.706 kb on + strand at 731.992 kb on - strand at 732.078 kb on + strand at 732.079 kb on - strand at 732.161 kb on + strand at 732.161 kb on + strand at 732.208 kb on - strand at 732.280 kb on + strand, within EX28DRAFT_0734 at 732.282 kb on + strand, within EX28DRAFT_0734 at 732.282 kb on + strand, within EX28DRAFT_0734 at 732.282 kb on + strand, within EX28DRAFT_0734 at 732.282 kb on + strand, within EX28DRAFT_0734 at 732.282 kb on + strand, within EX28DRAFT_0734 at 732.286 kb on - strand, within EX28DRAFT_0734 at 732.300 kb on + strand, within EX28DRAFT_0734 at 732.377 kb on + strand at 732.389 kb on + strand at 732.457 kb on + strand at 732.569 kb on + strand at 732.602 kb on - strand at 732.621 kb on + strand at 732.659 kb on - strand at 732.670 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 729,704 + +0.5 729,872 - EX28DRAFT_0730 0.47 -1.5 729,875 + EX28DRAFT_0730 0.48 -2.9 729,975 + EX28DRAFT_0730 0.90 +0.1 730,022 + +0.1 730,034 - +0.6 730,034 - -0.5 730,100 - +0.2 730,133 + -1.0 730,305 + +0.3 730,305 + -1.0 730,625 - EX28DRAFT_0732 0.85 -0.0 730,625 - EX28DRAFT_0732 0.85 +0.2 730,960 - EX28DRAFT_0733 0.27 -0.8 730,968 + EX28DRAFT_0733 0.28 -0.3 731,238 + EX28DRAFT_0733 0.55 +1.2 731,238 + EX28DRAFT_0733 0.55 +0.0 731,267 + EX28DRAFT_0733 0.58 -3.6 731,381 - EX28DRAFT_0733 0.69 +0.2 731,422 - EX28DRAFT_0733 0.74 -0.6 731,444 + EX28DRAFT_0733 0.76 +0.6 731,453 - EX28DRAFT_0733 0.77 +3.5 731,484 + EX28DRAFT_0733 0.80 +0.4 731,668 + +0.6 731,697 + -0.0 731,704 + +0.8 731,706 + +0.4 731,706 + -0.9 731,992 - +1.3 732,078 + -0.4 732,079 - -1.4 732,161 + +0.2 732,161 + -1.8 732,208 - -1.0 732,280 + EX28DRAFT_0734 0.20 +1.1 732,282 + EX28DRAFT_0734 0.20 -1.1 732,282 + EX28DRAFT_0734 0.20 +0.4 732,282 + EX28DRAFT_0734 0.20 +0.8 732,282 + EX28DRAFT_0734 0.20 +0.3 732,282 + EX28DRAFT_0734 0.20 -1.2 732,286 - EX28DRAFT_0734 0.21 -0.3 732,300 + EX28DRAFT_0734 0.24 +0.1 732,377 + +1.0 732,389 + -2.1 732,457 + -0.1 732,569 + -0.2 732,602 - +0.2 732,621 + -0.3 732,659 - -0.3 732,670 + +1.0
Or see this region's nucleotide sequence