Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0619 and EX28DRAFT_0620 are separated by 48 nucleotides EX28DRAFT_0620 and EX28DRAFT_0621 are separated by 5 nucleotides EX28DRAFT_0621 and EX28DRAFT_0622 are separated by 21 nucleotides EX28DRAFT_0622 and EX28DRAFT_0623 are separated by 400 nucleotides
EX28DRAFT_0619: EX28DRAFT_0619 - Glycosidases, at 616,566 to 618,056
_0619
EX28DRAFT_0620: EX28DRAFT_0620 - Flagellar protein FliT, at 618,105 to 618,479
_0620
EX28DRAFT_0621: EX28DRAFT_0621 - flagellar biosynthetic protein FliS, at 618,485 to 618,889
_0621
EX28DRAFT_0622: EX28DRAFT_0622 - Flagellar capping protein, at 618,911 to 620,335
_0622
EX28DRAFT_0623: EX28DRAFT_0623 - Bacterial flagellin N-terminal helical region/Bacterial flagellin C-terminal helical region, at 620,736 to 621,560
_0623
Position (kb) 618
619
620
621 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 617.918 kb on + strand at 618.016 kb on + strand at 618.016 kb on + strand at 618.017 kb on - strand at 618.033 kb on + strand at 618.034 kb on - strand at 618.034 kb on - strand at 618.091 kb on + strand at 618.109 kb on - strand at 618.146 kb on + strand, within EX28DRAFT_0620 at 618.171 kb on - strand, within EX28DRAFT_0620 at 618.362 kb on - strand, within EX28DRAFT_0620 at 618.473 kb on + strand at 618.548 kb on + strand, within EX28DRAFT_0621 at 618.575 kb on + strand, within EX28DRAFT_0621 at 618.617 kb on - strand, within EX28DRAFT_0621 at 618.618 kb on + strand, within EX28DRAFT_0621 at 618.780 kb on + strand, within EX28DRAFT_0621 at 618.782 kb on - strand, within EX28DRAFT_0621 at 618.845 kb on + strand, within EX28DRAFT_0621 at 618.851 kb on + strand at 618.883 kb on + strand at 618.884 kb on - strand at 618.884 kb on - strand at 618.886 kb on - strand at 618.886 kb on - strand at 618.886 kb on - strand at 618.886 kb on - strand at 618.886 kb on - strand at 618.890 kb on - strand at 618.907 kb on - strand at 618.907 kb on - strand at 618.940 kb on - strand at 618.968 kb on + strand at 618.971 kb on + strand at 619.041 kb on + strand at 619.449 kb on + strand, within EX28DRAFT_0622 at 619.450 kb on - strand, within EX28DRAFT_0622 at 619.450 kb on - strand, within EX28DRAFT_0622 at 619.450 kb on - strand, within EX28DRAFT_0622 at 619.450 kb on - strand, within EX28DRAFT_0622 at 619.489 kb on - strand, within EX28DRAFT_0622 at 619.955 kb on - strand, within EX28DRAFT_0622 at 619.964 kb on - strand, within EX28DRAFT_0622 at 619.964 kb on - strand, within EX28DRAFT_0622 at 619.973 kb on - strand, within EX28DRAFT_0622 at 620.092 kb on + strand, within EX28DRAFT_0622 at 620.208 kb on - strand at 620.221 kb on - strand at 620.283 kb on + strand at 620.284 kb on - strand at 620.284 kb on - strand at 620.284 kb on - strand at 620.285 kb on + strand at 620.286 kb on - strand at 620.429 kb on + strand at 620.449 kb on - strand at 620.469 kb on - strand at 620.546 kb on - strand at 620.750 kb on + strand at 620.750 kb on + strand at 620.754 kb on - strand at 620.810 kb on + strand at 621.162 kb on - strand, within EX28DRAFT_0623 at 621.193 kb on + strand, within EX28DRAFT_0623
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 617,918 + -0.6 618,016 + +0.5 618,016 + +0.9 618,017 - +0.4 618,033 + -2.2 618,034 - +0.3 618,034 - -2.5 618,091 + -0.5 618,109 - +0.5 618,146 + EX28DRAFT_0620 0.11 -3.0 618,171 - EX28DRAFT_0620 0.18 -1.2 618,362 - EX28DRAFT_0620 0.69 +0.5 618,473 + +1.0 618,548 + EX28DRAFT_0621 0.16 -1.3 618,575 + EX28DRAFT_0621 0.22 +0.1 618,617 - EX28DRAFT_0621 0.33 -0.0 618,618 + EX28DRAFT_0621 0.33 -0.8 618,780 + EX28DRAFT_0621 0.73 -1.2 618,782 - EX28DRAFT_0621 0.73 +0.1 618,845 + EX28DRAFT_0621 0.89 +0.5 618,851 + +0.7 618,883 + -0.8 618,884 - -1.3 618,884 - +0.2 618,886 - +1.1 618,886 - -0.1 618,886 - +0.1 618,886 - -0.4 618,886 - -0.2 618,890 - -0.8 618,907 - +1.0 618,907 - -0.9 618,940 - +1.3 618,968 + +0.7 618,971 + +0.5 619,041 + +0.2 619,449 + EX28DRAFT_0622 0.38 +0.5 619,450 - EX28DRAFT_0622 0.38 -1.3 619,450 - EX28DRAFT_0622 0.38 -1.6 619,450 - EX28DRAFT_0622 0.38 -0.2 619,450 - EX28DRAFT_0622 0.38 +0.2 619,489 - EX28DRAFT_0622 0.41 -0.2 619,955 - EX28DRAFT_0622 0.73 -1.2 619,964 - EX28DRAFT_0622 0.74 -1.4 619,964 - EX28DRAFT_0622 0.74 +0.3 619,973 - EX28DRAFT_0622 0.75 -0.4 620,092 + EX28DRAFT_0622 0.83 -0.9 620,208 - +0.8 620,221 - -0.4 620,283 + -0.4 620,284 - -0.5 620,284 - +1.5 620,284 - +0.1 620,285 + +1.5 620,286 - +0.9 620,429 + +1.2 620,449 - -0.2 620,469 - +0.2 620,546 - -0.1 620,750 + -0.1 620,750 + -0.6 620,754 - -1.2 620,810 + -0.0 621,162 - EX28DRAFT_0623 0.52 -2.2 621,193 + EX28DRAFT_0623 0.55 -0.1
Or see this region's nucleotide sequence