Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0596 and EX28DRAFT_0597 are separated by 78 nucleotides EX28DRAFT_0597 and EX28DRAFT_0598 overlap by 47 nucleotides EX28DRAFT_0598 and EX28DRAFT_0599 overlap by 4 nucleotides
EX28DRAFT_0596: EX28DRAFT_0596 - Uncharacterized protein conserved in bacteria, at 599,218 to 600,129
_0596
EX28DRAFT_0597: EX28DRAFT_0597 - Uncharacterized small protein, at 600,208 to 600,390
_0597
EX28DRAFT_0598: EX28DRAFT_0598 - diguanylate cyclase (GGDEF) domain, at 600,344 to 602,152
_0598
EX28DRAFT_0599: EX28DRAFT_0599 - mannosyl-3-phosphoglycerate phosphatase-related protein, at 602,149 to 602,949
_0599
Position (kb)
600
601
602
603 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 599.421 kb on + strand, within EX28DRAFT_0596 at 599.422 kb on - strand, within EX28DRAFT_0596 at 599.525 kb on + strand, within EX28DRAFT_0596 at 599.526 kb on - strand, within EX28DRAFT_0596 at 599.568 kb on + strand, within EX28DRAFT_0596 at 599.568 kb on + strand, within EX28DRAFT_0596 at 599.569 kb on - strand, within EX28DRAFT_0596 at 599.780 kb on - strand, within EX28DRAFT_0596 at 600.111 kb on - strand at 600.127 kb on + strand at 600.127 kb on + strand at 600.127 kb on + strand at 600.195 kb on - strand at 600.207 kb on + strand at 600.208 kb on - strand at 600.235 kb on - strand, within EX28DRAFT_0597 at 600.241 kb on - strand, within EX28DRAFT_0597 at 600.258 kb on + strand, within EX28DRAFT_0597 at 600.258 kb on + strand, within EX28DRAFT_0597 at 600.258 kb on + strand, within EX28DRAFT_0597 at 600.259 kb on - strand, within EX28DRAFT_0597 at 600.259 kb on - strand, within EX28DRAFT_0597 at 600.259 kb on - strand, within EX28DRAFT_0597 at 600.259 kb on - strand, within EX28DRAFT_0597 at 600.388 kb on + strand at 600.389 kb on - strand at 600.396 kb on + strand at 600.431 kb on + strand at 600.486 kb on + strand at 600.487 kb on - strand at 600.567 kb on + strand, within EX28DRAFT_0598 at 600.617 kb on + strand, within EX28DRAFT_0598 at 600.618 kb on - strand, within EX28DRAFT_0598 at 600.618 kb on - strand, within EX28DRAFT_0598 at 600.618 kb on - strand, within EX28DRAFT_0598 at 600.634 kb on + strand, within EX28DRAFT_0598 at 600.816 kb on + strand, within EX28DRAFT_0598 at 600.989 kb on + strand, within EX28DRAFT_0598 at 600.990 kb on - strand, within EX28DRAFT_0598 at 600.990 kb on - strand, within EX28DRAFT_0598 at 601.049 kb on - strand, within EX28DRAFT_0598 at 601.061 kb on + strand, within EX28DRAFT_0598 at 601.171 kb on + strand, within EX28DRAFT_0598 at 601.172 kb on - strand, within EX28DRAFT_0598 at 601.196 kb on + strand, within EX28DRAFT_0598 at 601.197 kb on - strand, within EX28DRAFT_0598 at 601.238 kb on - strand, within EX28DRAFT_0598 at 601.323 kb on + strand, within EX28DRAFT_0598 at 601.324 kb on + strand, within EX28DRAFT_0598 at 601.325 kb on - strand, within EX28DRAFT_0598 at 601.571 kb on - strand, within EX28DRAFT_0598 at 601.581 kb on + strand, within EX28DRAFT_0598 at 601.582 kb on - strand, within EX28DRAFT_0598 at 601.625 kb on + strand, within EX28DRAFT_0598 at 601.625 kb on + strand, within EX28DRAFT_0598 at 601.626 kb on - strand, within EX28DRAFT_0598 at 601.626 kb on - strand, within EX28DRAFT_0598 at 601.783 kb on + strand, within EX28DRAFT_0598 at 601.960 kb on + strand, within EX28DRAFT_0598 at 601.981 kb on + strand at 602.064 kb on - strand at 602.266 kb on - strand, within EX28DRAFT_0599 at 602.513 kb on - strand, within EX28DRAFT_0599 at 602.619 kb on + strand, within EX28DRAFT_0599 at 602.711 kb on - strand, within EX28DRAFT_0599 at 602.836 kb on - strand, within EX28DRAFT_0599 at 602.879 kb on + strand at 602.879 kb on + strand at 602.879 kb on + strand at 602.880 kb on - strand at 602.880 kb on - strand at 602.880 kb on - strand at 602.880 kb on - strand at 602.931 kb on + strand at 602.931 kb on + strand at 602.932 kb on - strand at 603.110 kb on - strand at 603.115 kb on + strand at 603.117 kb on + strand at 603.145 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 599,421 + EX28DRAFT_0596 0.22 +0.5 599,422 - EX28DRAFT_0596 0.22 +0.4 599,525 + EX28DRAFT_0596 0.34 -0.6 599,526 - EX28DRAFT_0596 0.34 +0.7 599,568 + EX28DRAFT_0596 0.38 +0.1 599,568 + EX28DRAFT_0596 0.38 +0.5 599,569 - EX28DRAFT_0596 0.38 +0.5 599,780 - EX28DRAFT_0596 0.62 -0.2 600,111 - +0.4 600,127 + +0.7 600,127 + +0.7 600,127 + +0.2 600,195 - -1.3 600,207 + +1.5 600,208 - +2.3 600,235 - EX28DRAFT_0597 0.15 +0.2 600,241 - EX28DRAFT_0597 0.18 +0.6 600,258 + EX28DRAFT_0597 0.27 +1.0 600,258 + EX28DRAFT_0597 0.27 -0.2 600,258 + EX28DRAFT_0597 0.27 -1.7 600,259 - EX28DRAFT_0597 0.28 -0.2 600,259 - EX28DRAFT_0597 0.28 -0.5 600,259 - EX28DRAFT_0597 0.28 +0.0 600,259 - EX28DRAFT_0597 0.28 -0.4 600,388 + -0.8 600,389 - +0.1 600,396 + +0.0 600,431 + -0.8 600,486 + +0.0 600,487 - -0.4 600,567 + EX28DRAFT_0598 0.12 +1.8 600,617 + EX28DRAFT_0598 0.15 +0.0 600,618 - EX28DRAFT_0598 0.15 -0.1 600,618 - EX28DRAFT_0598 0.15 +0.3 600,618 - EX28DRAFT_0598 0.15 -1.2 600,634 + EX28DRAFT_0598 0.16 +0.9 600,816 + EX28DRAFT_0598 0.26 -1.0 600,989 + EX28DRAFT_0598 0.36 -0.1 600,990 - EX28DRAFT_0598 0.36 +3.6 600,990 - EX28DRAFT_0598 0.36 -1.8 601,049 - EX28DRAFT_0598 0.39 -1.5 601,061 + EX28DRAFT_0598 0.40 -1.4 601,171 + EX28DRAFT_0598 0.46 -0.5 601,172 - EX28DRAFT_0598 0.46 -0.0 601,196 + EX28DRAFT_0598 0.47 -1.6 601,197 - EX28DRAFT_0598 0.47 +2.0 601,238 - EX28DRAFT_0598 0.49 +1.2 601,323 + EX28DRAFT_0598 0.54 +0.7 601,324 + EX28DRAFT_0598 0.54 -1.2 601,325 - EX28DRAFT_0598 0.54 +1.3 601,571 - EX28DRAFT_0598 0.68 +1.8 601,581 + EX28DRAFT_0598 0.68 +0.3 601,582 - EX28DRAFT_0598 0.68 +0.4 601,625 + EX28DRAFT_0598 0.71 +0.4 601,625 + EX28DRAFT_0598 0.71 +0.1 601,626 - EX28DRAFT_0598 0.71 +0.1 601,626 - EX28DRAFT_0598 0.71 -0.5 601,783 + EX28DRAFT_0598 0.80 +0.6 601,960 + EX28DRAFT_0598 0.89 +0.5 601,981 + -2.8 602,064 - +0.7 602,266 - EX28DRAFT_0599 0.15 -2.2 602,513 - EX28DRAFT_0599 0.45 -0.4 602,619 + EX28DRAFT_0599 0.59 -0.4 602,711 - EX28DRAFT_0599 0.70 +0.2 602,836 - EX28DRAFT_0599 0.86 +1.7 602,879 + +0.1 602,879 + -0.3 602,879 + +0.2 602,880 - -3.4 602,880 - -1.2 602,880 - -0.8 602,880 - +0.5 602,931 + +0.0 602,931 + -0.0 602,932 - -3.5 603,110 - +0.5 603,115 + +0.1 603,117 + -1.8 603,145 + -2.5
Or see this region's nucleotide sequence