Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0459 and EX28DRAFT_0460 are separated by 300 nucleotides EX28DRAFT_0460 and EX28DRAFT_0461 are separated by 136 nucleotides
EX28DRAFT_0459: EX28DRAFT_0459 - Superfamily I DNA and RNA helicases, at 443,269 to 445,050
_0459
EX28DRAFT_0460: EX28DRAFT_0460 - hypothetical protein, at 445,351 to 445,593
_0460
EX28DRAFT_0461: EX28DRAFT_0461 - tRNA_anti-like, at 445,730 to 446,512
_0461
Position (kb) 445
446 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 444.528 kb on + strand, within EX28DRAFT_0459 at 444.529 kb on - strand, within EX28DRAFT_0459 at 444.598 kb on + strand, within EX28DRAFT_0459 at 444.710 kb on + strand, within EX28DRAFT_0459 at 444.739 kb on + strand, within EX28DRAFT_0459 at 444.740 kb on - strand, within EX28DRAFT_0459 at 444.740 kb on - strand, within EX28DRAFT_0459 at 444.740 kb on - strand, within EX28DRAFT_0459 at 444.740 kb on - strand, within EX28DRAFT_0459 at 444.744 kb on + strand, within EX28DRAFT_0459 at 444.919 kb on + strand at 444.920 kb on - strand at 444.923 kb on + strand at 444.923 kb on + strand at 444.924 kb on - strand at 444.924 kb on - strand at 444.997 kb on + strand at 444.997 kb on + strand at 444.999 kb on + strand at 445.000 kb on - strand at 445.000 kb on - strand at 445.036 kb on + strand at 445.037 kb on - strand at 445.061 kb on + strand at 445.066 kb on + strand at 445.230 kb on - strand at 445.230 kb on - strand at 445.243 kb on - strand at 445.288 kb on - strand at 445.293 kb on + strand at 445.332 kb on + strand at 445.333 kb on - strand at 445.333 kb on - strand at 445.333 kb on - strand at 445.336 kb on + strand at 445.336 kb on + strand at 445.384 kb on - strand, within EX28DRAFT_0460 at 445.384 kb on - strand, within EX28DRAFT_0460 at 445.416 kb on + strand, within EX28DRAFT_0460 at 445.417 kb on - strand, within EX28DRAFT_0460 at 445.424 kb on + strand, within EX28DRAFT_0460 at 445.425 kb on - strand, within EX28DRAFT_0460 at 445.425 kb on - strand, within EX28DRAFT_0460 at 445.428 kb on + strand, within EX28DRAFT_0460 at 445.428 kb on + strand, within EX28DRAFT_0460 at 445.429 kb on - strand, within EX28DRAFT_0460 at 445.431 kb on + strand, within EX28DRAFT_0460 at 445.431 kb on + strand, within EX28DRAFT_0460 at 445.496 kb on - strand, within EX28DRAFT_0460 at 445.541 kb on - strand, within EX28DRAFT_0460 at 445.550 kb on + strand, within EX28DRAFT_0460 at 445.550 kb on + strand, within EX28DRAFT_0460 at 445.551 kb on - strand, within EX28DRAFT_0460 at 445.551 kb on - strand, within EX28DRAFT_0460 at 445.741 kb on + strand at 445.823 kb on + strand, within EX28DRAFT_0461 at 445.835 kb on + strand, within EX28DRAFT_0461 at 445.835 kb on + strand, within EX28DRAFT_0461 at 445.836 kb on - strand, within EX28DRAFT_0461 at 445.836 kb on - strand, within EX28DRAFT_0461 at 445.836 kb on - strand, within EX28DRAFT_0461 at 445.873 kb on - strand, within EX28DRAFT_0461 at 445.965 kb on - strand, within EX28DRAFT_0461 at 445.977 kb on - strand, within EX28DRAFT_0461 at 445.984 kb on + strand, within EX28DRAFT_0461 at 445.985 kb on - strand, within EX28DRAFT_0461 at 445.985 kb on - strand, within EX28DRAFT_0461 at 445.985 kb on - strand, within EX28DRAFT_0461 at 446.207 kb on + strand, within EX28DRAFT_0461 at 446.240 kb on + strand, within EX28DRAFT_0461 at 446.284 kb on + strand, within EX28DRAFT_0461 at 446.285 kb on - strand, within EX28DRAFT_0461 at 446.316 kb on + strand, within EX28DRAFT_0461 at 446.316 kb on + strand, within EX28DRAFT_0461 at 446.317 kb on - strand, within EX28DRAFT_0461 at 446.317 kb on - strand, within EX28DRAFT_0461 at 446.347 kb on + strand, within EX28DRAFT_0461 at 446.500 kb on + strand at 446.500 kb on + strand at 446.506 kb on + strand at 446.552 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 444,528 + EX28DRAFT_0459 0.71 +0.1 444,529 - EX28DRAFT_0459 0.71 +0.2 444,598 + EX28DRAFT_0459 0.75 +0.3 444,710 + EX28DRAFT_0459 0.81 -0.6 444,739 + EX28DRAFT_0459 0.82 -1.4 444,740 - EX28DRAFT_0459 0.83 -0.8 444,740 - EX28DRAFT_0459 0.83 +0.8 444,740 - EX28DRAFT_0459 0.83 -0.7 444,740 - EX28DRAFT_0459 0.83 -0.3 444,744 + EX28DRAFT_0459 0.83 -0.2 444,919 + +0.4 444,920 - +0.4 444,923 + -1.3 444,923 + -0.8 444,924 - -1.7 444,924 - -0.1 444,997 + -1.5 444,997 + -0.2 444,999 + +1.1 445,000 - -1.1 445,000 - +0.7 445,036 + -0.7 445,037 - +0.9 445,061 + +0.9 445,066 + -1.1 445,230 - -0.1 445,230 - +0.5 445,243 - -0.3 445,288 - +0.8 445,293 + +0.2 445,332 + -0.5 445,333 - +0.8 445,333 - +1.1 445,333 - +2.2 445,336 + -2.4 445,336 + -0.7 445,384 - EX28DRAFT_0460 0.14 -1.7 445,384 - EX28DRAFT_0460 0.14 +1.2 445,416 + EX28DRAFT_0460 0.27 +0.4 445,417 - EX28DRAFT_0460 0.27 -0.0 445,424 + EX28DRAFT_0460 0.30 -0.5 445,425 - EX28DRAFT_0460 0.30 +0.8 445,425 - EX28DRAFT_0460 0.30 +0.5 445,428 + EX28DRAFT_0460 0.32 +0.6 445,428 + EX28DRAFT_0460 0.32 -0.3 445,429 - EX28DRAFT_0460 0.32 +0.7 445,431 + EX28DRAFT_0460 0.33 -2.1 445,431 + EX28DRAFT_0460 0.33 -0.2 445,496 - EX28DRAFT_0460 0.60 -0.1 445,541 - EX28DRAFT_0460 0.78 -0.4 445,550 + EX28DRAFT_0460 0.82 +0.2 445,550 + EX28DRAFT_0460 0.82 -0.1 445,551 - EX28DRAFT_0460 0.82 -0.2 445,551 - EX28DRAFT_0460 0.82 +0.7 445,741 + -0.5 445,823 + EX28DRAFT_0461 0.12 -0.5 445,835 + EX28DRAFT_0461 0.13 -0.3 445,835 + EX28DRAFT_0461 0.13 -0.6 445,836 - EX28DRAFT_0461 0.14 +0.1 445,836 - EX28DRAFT_0461 0.14 -3.1 445,836 - EX28DRAFT_0461 0.14 +1.3 445,873 - EX28DRAFT_0461 0.18 +0.0 445,965 - EX28DRAFT_0461 0.30 -1.2 445,977 - EX28DRAFT_0461 0.32 -0.4 445,984 + EX28DRAFT_0461 0.32 -0.6 445,985 - EX28DRAFT_0461 0.33 +0.6 445,985 - EX28DRAFT_0461 0.33 +0.6 445,985 - EX28DRAFT_0461 0.33 -0.2 446,207 + EX28DRAFT_0461 0.61 -0.8 446,240 + EX28DRAFT_0461 0.65 +0.0 446,284 + EX28DRAFT_0461 0.71 +0.8 446,285 - EX28DRAFT_0461 0.71 +0.3 446,316 + EX28DRAFT_0461 0.75 -1.7 446,316 + EX28DRAFT_0461 0.75 +1.0 446,317 - EX28DRAFT_0461 0.75 -0.3 446,317 - EX28DRAFT_0461 0.75 -1.7 446,347 + EX28DRAFT_0461 0.79 +0.6 446,500 + +1.5 446,500 + +0.3 446,506 + -0.3 446,552 + -0.3
Or see this region's nucleotide sequence