Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0198 and EX28DRAFT_0199 are separated by 56 nucleotides EX28DRAFT_0199 and EX28DRAFT_0200 overlap by 1 nucleotides
EX28DRAFT_0198: EX28DRAFT_0198 - Malic enzyme, at 152,558 to 154,837
_0198
EX28DRAFT_0199: EX28DRAFT_0199 - coproporphyrinogen oxidase (EC 1.3.3.3), at 154,894 to 155,793
_0199
EX28DRAFT_0200: EX28DRAFT_0200 - N-acetylmuramoyl-L-alanine amidase, at 155,793 to 156,668
_0200
Position (kb) 154
155
156 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 153.951 kb on + strand, within EX28DRAFT_0198 at 154.097 kb on + strand, within EX28DRAFT_0198 at 154.098 kb on - strand, within EX28DRAFT_0198 at 154.100 kb on + strand, within EX28DRAFT_0198 at 154.101 kb on - strand, within EX28DRAFT_0198 at 154.352 kb on + strand, within EX28DRAFT_0198 at 154.352 kb on + strand, within EX28DRAFT_0198 at 154.353 kb on - strand, within EX28DRAFT_0198 at 154.353 kb on - strand, within EX28DRAFT_0198 at 154.353 kb on - strand, within EX28DRAFT_0198 at 154.477 kb on + strand, within EX28DRAFT_0198 at 154.478 kb on - strand, within EX28DRAFT_0198 at 154.603 kb on + strand, within EX28DRAFT_0198 at 154.636 kb on + strand at 154.645 kb on + strand at 154.676 kb on + strand at 154.716 kb on + strand at 154.784 kb on - strand at 154.836 kb on - strand at 154.895 kb on + strand at 154.896 kb on - strand at 154.896 kb on - strand at 154.896 kb on - strand at 154.967 kb on + strand at 155.169 kb on - strand, within EX28DRAFT_0199 at 155.391 kb on - strand, within EX28DRAFT_0199 at 155.846 kb on + strand at 156.027 kb on - strand, within EX28DRAFT_0200 at 156.027 kb on - strand, within EX28DRAFT_0200 at 156.041 kb on + strand, within EX28DRAFT_0200 at 156.042 kb on - strand, within EX28DRAFT_0200 at 156.075 kb on - strand, within EX28DRAFT_0200 at 156.109 kb on - strand, within EX28DRAFT_0200 at 156.252 kb on - strand, within EX28DRAFT_0200 at 156.252 kb on - strand, within EX28DRAFT_0200 at 156.275 kb on + strand, within EX28DRAFT_0200 at 156.276 kb on - strand, within EX28DRAFT_0200 at 156.276 kb on - strand, within EX28DRAFT_0200 at 156.319 kb on + strand, within EX28DRAFT_0200 at 156.319 kb on + strand, within EX28DRAFT_0200 at 156.680 kb on - strand at 156.703 kb on + strand at 156.704 kb on - strand at 156.705 kb on + strand at 156.706 kb on - strand at 156.711 kb on - strand at 156.768 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 153,951 + EX28DRAFT_0198 0.61 +0.1 154,097 + EX28DRAFT_0198 0.68 -1.2 154,098 - EX28DRAFT_0198 0.68 -0.9 154,100 + EX28DRAFT_0198 0.68 +1.0 154,101 - EX28DRAFT_0198 0.68 +0.4 154,352 + EX28DRAFT_0198 0.79 -0.4 154,352 + EX28DRAFT_0198 0.79 +0.6 154,353 - EX28DRAFT_0198 0.79 +0.3 154,353 - EX28DRAFT_0198 0.79 -0.0 154,353 - EX28DRAFT_0198 0.79 -0.4 154,477 + EX28DRAFT_0198 0.84 -0.2 154,478 - EX28DRAFT_0198 0.84 -1.6 154,603 + EX28DRAFT_0198 0.90 -0.0 154,636 + +0.3 154,645 + -0.2 154,676 + -2.3 154,716 + +0.1 154,784 - +1.0 154,836 - +0.6 154,895 + +0.0 154,896 - -0.2 154,896 - -0.6 154,896 - +1.1 154,967 + -2.5 155,169 - EX28DRAFT_0199 0.31 +0.1 155,391 - EX28DRAFT_0199 0.55 +1.2 155,846 + -0.4 156,027 - EX28DRAFT_0200 0.27 -0.9 156,027 - EX28DRAFT_0200 0.27 -0.0 156,041 + EX28DRAFT_0200 0.28 -0.8 156,042 - EX28DRAFT_0200 0.28 -0.4 156,075 - EX28DRAFT_0200 0.32 -1.3 156,109 - EX28DRAFT_0200 0.36 +1.1 156,252 - EX28DRAFT_0200 0.52 -0.3 156,252 - EX28DRAFT_0200 0.52 +0.6 156,275 + EX28DRAFT_0200 0.55 +0.6 156,276 - EX28DRAFT_0200 0.55 +0.5 156,276 - EX28DRAFT_0200 0.55 +0.6 156,319 + EX28DRAFT_0200 0.60 -0.6 156,319 + EX28DRAFT_0200 0.60 -0.0 156,680 - -0.3 156,703 + -2.9 156,704 - +0.3 156,705 + -0.4 156,706 - -0.7 156,711 - +0.4 156,768 - +0.6
Or see this region's nucleotide sequence