Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0193 and EX28DRAFT_0194 are separated by 66 nucleotides EX28DRAFT_0194 and EX28DRAFT_0195 are separated by 101 nucleotides EX28DRAFT_0195 and EX28DRAFT_0196 are separated by 96 nucleotides
EX28DRAFT_0193: EX28DRAFT_0193 - glutamate synthase small subunit family protein, proteobacterial, at 145,432 to 147,408
_0193
EX28DRAFT_0194: EX28DRAFT_0194 - nudix-type nucleoside diphosphatase, YffH/AdpP family, at 147,475 to 148,068
_0194
EX28DRAFT_0195: EX28DRAFT_0195 - Protein of unknown function (DUF1176), at 148,170 to 149,213
_0195
EX28DRAFT_0196: EX28DRAFT_0196 - transketolase, bacterial and yeast, at 149,310 to 151,298
_0196
Position (kb) 148
149
150 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 147.406 kb on + strand at 147.441 kb on - strand at 147.441 kb on - strand at 147.443 kb on + strand at 147.444 kb on - strand at 147.444 kb on - strand at 147.445 kb on + strand at 147.446 kb on - strand at 147.523 kb on + strand at 147.537 kb on + strand, within EX28DRAFT_0194 at 147.547 kb on + strand, within EX28DRAFT_0194 at 147.547 kb on + strand, within EX28DRAFT_0194 at 147.555 kb on + strand, within EX28DRAFT_0194 at 147.598 kb on + strand, within EX28DRAFT_0194 at 147.599 kb on - strand, within EX28DRAFT_0194 at 147.631 kb on + strand, within EX28DRAFT_0194 at 147.631 kb on + strand, within EX28DRAFT_0194 at 147.631 kb on + strand, within EX28DRAFT_0194 at 147.632 kb on - strand, within EX28DRAFT_0194 at 147.632 kb on - strand, within EX28DRAFT_0194 at 147.827 kb on + strand, within EX28DRAFT_0194 at 147.828 kb on - strand, within EX28DRAFT_0194 at 147.828 kb on - strand, within EX28DRAFT_0194 at 147.831 kb on + strand, within EX28DRAFT_0194 at 147.831 kb on + strand, within EX28DRAFT_0194 at 147.831 kb on + strand, within EX28DRAFT_0194 at 147.832 kb on - strand, within EX28DRAFT_0194 at 147.832 kb on - strand, within EX28DRAFT_0194 at 147.881 kb on - strand, within EX28DRAFT_0194 at 147.999 kb on + strand, within EX28DRAFT_0194 at 148.067 kb on - strand at 148.084 kb on + strand at 148.092 kb on - strand at 148.092 kb on - strand at 148.128 kb on + strand at 148.268 kb on - strand at 148.500 kb on - strand, within EX28DRAFT_0195 at 148.500 kb on - strand, within EX28DRAFT_0195 at 148.577 kb on + strand, within EX28DRAFT_0195 at 148.786 kb on - strand, within EX28DRAFT_0195 at 148.906 kb on - strand, within EX28DRAFT_0195 at 148.908 kb on - strand, within EX28DRAFT_0195 at 149.217 kb on + strand at 149.437 kb on + strand at 149.437 kb on + strand at 149.437 kb on + strand at 149.437 kb on + strand at 149.438 kb on - strand at 149.438 kb on - strand at 149.438 kb on - strand at 149.680 kb on + strand, within EX28DRAFT_0196 at 149.680 kb on + strand, within EX28DRAFT_0196 at 149.927 kb on - strand, within EX28DRAFT_0196 at 149.927 kb on - strand, within EX28DRAFT_0196 at 150.013 kb on + strand, within EX28DRAFT_0196 at 150.063 kb on - strand, within EX28DRAFT_0196 at 150.091 kb on + strand, within EX28DRAFT_0196 at 150.091 kb on + strand, within EX28DRAFT_0196 at 150.091 kb on + strand, within EX28DRAFT_0196 at 150.091 kb on + strand, within EX28DRAFT_0196
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 147,406 + +0.8 147,441 - +0.7 147,441 - +0.1 147,443 + -0.4 147,444 - -1.9 147,444 - +0.3 147,445 + -0.2 147,446 - +0.1 147,523 + -0.9 147,537 + EX28DRAFT_0194 0.10 +0.8 147,547 + EX28DRAFT_0194 0.12 +0.6 147,547 + EX28DRAFT_0194 0.12 +2.1 147,555 + EX28DRAFT_0194 0.13 -0.1 147,598 + EX28DRAFT_0194 0.21 +0.1 147,599 - EX28DRAFT_0194 0.21 +0.3 147,631 + EX28DRAFT_0194 0.26 +1.2 147,631 + EX28DRAFT_0194 0.26 -0.1 147,631 + EX28DRAFT_0194 0.26 -0.4 147,632 - EX28DRAFT_0194 0.26 +0.3 147,632 - EX28DRAFT_0194 0.26 -0.7 147,827 + EX28DRAFT_0194 0.59 -1.8 147,828 - EX28DRAFT_0194 0.59 -0.5 147,828 - EX28DRAFT_0194 0.59 +0.3 147,831 + EX28DRAFT_0194 0.60 -0.7 147,831 + EX28DRAFT_0194 0.60 +0.1 147,831 + EX28DRAFT_0194 0.60 -0.2 147,832 - EX28DRAFT_0194 0.60 +0.7 147,832 - EX28DRAFT_0194 0.60 +0.1 147,881 - EX28DRAFT_0194 0.68 +1.1 147,999 + EX28DRAFT_0194 0.88 -0.0 148,067 - +0.2 148,084 + -0.6 148,092 - +0.1 148,092 - -0.2 148,128 + +0.6 148,268 - -0.1 148,500 - EX28DRAFT_0195 0.32 -0.2 148,500 - EX28DRAFT_0195 0.32 +1.1 148,577 + EX28DRAFT_0195 0.39 +0.2 148,786 - EX28DRAFT_0195 0.59 -0.4 148,906 - EX28DRAFT_0195 0.70 +1.6 148,908 - EX28DRAFT_0195 0.71 +0.1 149,217 + +0.3 149,437 + -0.9 149,437 + -0.2 149,437 + +0.1 149,437 + +0.1 149,438 - +0.5 149,438 - +0.2 149,438 - +0.8 149,680 + EX28DRAFT_0196 0.19 +0.5 149,680 + EX28DRAFT_0196 0.19 +0.1 149,927 - EX28DRAFT_0196 0.31 +0.2 149,927 - EX28DRAFT_0196 0.31 -3.5 150,013 + EX28DRAFT_0196 0.35 -0.6 150,063 - EX28DRAFT_0196 0.38 -0.4 150,091 + EX28DRAFT_0196 0.39 +0.2 150,091 + EX28DRAFT_0196 0.39 -0.4 150,091 + EX28DRAFT_0196 0.39 +0.2 150,091 + EX28DRAFT_0196 0.39 -0.4
Or see this region's nucleotide sequence