Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0159 and EX28DRAFT_0160 are separated by 11 nucleotides EX28DRAFT_0160 and EX28DRAFT_0161 are separated by 50 nucleotides EX28DRAFT_0161 and EX28DRAFT_0162 are separated by 97 nucleotides EX28DRAFT_0162 and EX28DRAFT_0163 overlap by 4 nucleotides
EX28DRAFT_0159: EX28DRAFT_0159 - Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily), at 105,974 to 106,717
_0159
EX28DRAFT_0160: EX28DRAFT_0160 - hypothetical protein, at 106,729 to 107,196
_0160
EX28DRAFT_0161: EX28DRAFT_0161 - Cyanate permease, at 107,247 to 108,476
_0161
EX28DRAFT_0162: EX28DRAFT_0162 - Transcriptional regulator, at 108,574 to 109,464
_0162
EX28DRAFT_0163: EX28DRAFT_0163 - Protein of unknown function (DUF2633), at 109,461 to 109,631
_0163
Position (kb) 107
108
109 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 106.289 kb on + strand, within EX28DRAFT_0159 at 106.289 kb on + strand, within EX28DRAFT_0159 at 106.289 kb on + strand, within EX28DRAFT_0159 at 106.289 kb on + strand, within EX28DRAFT_0159 at 106.290 kb on - strand, within EX28DRAFT_0159 at 106.290 kb on - strand, within EX28DRAFT_0159 at 106.310 kb on - strand, within EX28DRAFT_0159 at 106.442 kb on + strand, within EX28DRAFT_0159 at 106.597 kb on + strand, within EX28DRAFT_0159 at 106.651 kb on + strand at 106.701 kb on - strand at 106.816 kb on - strand, within EX28DRAFT_0160 at 106.816 kb on - strand, within EX28DRAFT_0160 at 107.248 kb on + strand at 107.261 kb on + strand at 107.322 kb on + strand at 107.322 kb on + strand at 107.426 kb on + strand, within EX28DRAFT_0161 at 107.506 kb on + strand, within EX28DRAFT_0161 at 107.507 kb on - strand, within EX28DRAFT_0161 at 107.609 kb on + strand, within EX28DRAFT_0161 at 107.609 kb on + strand, within EX28DRAFT_0161 at 107.609 kb on + strand, within EX28DRAFT_0161 at 107.609 kb on + strand, within EX28DRAFT_0161 at 107.783 kb on + strand, within EX28DRAFT_0161 at 108.102 kb on + strand, within EX28DRAFT_0161 at 108.103 kb on - strand, within EX28DRAFT_0161 at 108.173 kb on - strand, within EX28DRAFT_0161 at 108.335 kb on - strand, within EX28DRAFT_0161 at 108.398 kb on - strand at 108.428 kb on + strand at 108.428 kb on + strand at 108.428 kb on + strand at 108.492 kb on + strand at 108.524 kb on - strand at 108.549 kb on - strand at 108.557 kb on + strand at 108.559 kb on + strand at 108.689 kb on + strand, within EX28DRAFT_0162 at 108.861 kb on + strand, within EX28DRAFT_0162 at 108.861 kb on + strand, within EX28DRAFT_0162 at 108.862 kb on - strand, within EX28DRAFT_0162 at 108.862 kb on - strand, within EX28DRAFT_0162 at 109.084 kb on - strand, within EX28DRAFT_0162 at 109.293 kb on + strand, within EX28DRAFT_0162 at 109.293 kb on + strand, within EX28DRAFT_0162 at 109.293 kb on + strand, within EX28DRAFT_0162 at 109.294 kb on - strand, within EX28DRAFT_0162 at 109.388 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 106,289 + EX28DRAFT_0159 0.42 +0.4 106,289 + EX28DRAFT_0159 0.42 +0.1 106,289 + EX28DRAFT_0159 0.42 -1.6 106,289 + EX28DRAFT_0159 0.42 +0.2 106,290 - EX28DRAFT_0159 0.42 -0.1 106,290 - EX28DRAFT_0159 0.42 -1.1 106,310 - EX28DRAFT_0159 0.45 -0.2 106,442 + EX28DRAFT_0159 0.63 -1.3 106,597 + EX28DRAFT_0159 0.84 +0.8 106,651 + +0.1 106,701 - -2.0 106,816 - EX28DRAFT_0160 0.19 +0.7 106,816 - EX28DRAFT_0160 0.19 -0.3 107,248 + -0.5 107,261 + +1.7 107,322 + +2.1 107,322 + +0.4 107,426 + EX28DRAFT_0161 0.15 -1.0 107,506 + EX28DRAFT_0161 0.21 -0.0 107,507 - EX28DRAFT_0161 0.21 +0.3 107,609 + EX28DRAFT_0161 0.29 +0.3 107,609 + EX28DRAFT_0161 0.29 -0.5 107,609 + EX28DRAFT_0161 0.29 -3.5 107,609 + EX28DRAFT_0161 0.29 +0.3 107,783 + EX28DRAFT_0161 0.44 -0.9 108,102 + EX28DRAFT_0161 0.70 -0.0 108,103 - EX28DRAFT_0161 0.70 -0.3 108,173 - EX28DRAFT_0161 0.75 +0.1 108,335 - EX28DRAFT_0161 0.88 -0.1 108,398 - -0.1 108,428 + +0.5 108,428 + +0.9 108,428 + -0.6 108,492 + -3.9 108,524 - +1.0 108,549 - -0.6 108,557 + +0.9 108,559 + -0.2 108,689 + EX28DRAFT_0162 0.13 +0.4 108,861 + EX28DRAFT_0162 0.32 -1.1 108,861 + EX28DRAFT_0162 0.32 -0.2 108,862 - EX28DRAFT_0162 0.32 -1.3 108,862 - EX28DRAFT_0162 0.32 -0.7 109,084 - EX28DRAFT_0162 0.57 -2.5 109,293 + EX28DRAFT_0162 0.81 -0.6 109,293 + EX28DRAFT_0162 0.81 +0.2 109,293 + EX28DRAFT_0162 0.81 +0.2 109,294 - EX28DRAFT_0162 0.81 +0.4 109,388 + -0.1
Or see this region's nucleotide sequence