Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0147 and EX28DRAFT_0148 are separated by 11 nucleotides EX28DRAFT_0148 and EX28DRAFT_0149 are separated by 301 nucleotides
EX28DRAFT_0147: EX28DRAFT_0147 - methylmalonic acid semialdehyde dehydrogenase, at 91,102 to 92,607
_0147
EX28DRAFT_0148: EX28DRAFT_0148 - Acetolactate synthase, at 92,619 to 94,544
_0148
EX28DRAFT_0149: EX28DRAFT_0149 - Predicted dehydrogenases and related proteins, at 94,846 to 95,832
_0149
Position (kb) 92
93
94
95 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 91.802 kb on - strand, within EX28DRAFT_0147 at 91.977 kb on + strand, within EX28DRAFT_0147 at 92.113 kb on + strand, within EX28DRAFT_0147 at 92.195 kb on - strand, within EX28DRAFT_0147 at 92.195 kb on - strand, within EX28DRAFT_0147 at 92.245 kb on + strand, within EX28DRAFT_0147 at 92.245 kb on + strand, within EX28DRAFT_0147 at 92.246 kb on - strand, within EX28DRAFT_0147 at 92.246 kb on - strand, within EX28DRAFT_0147 at 92.332 kb on + strand, within EX28DRAFT_0147 at 92.332 kb on + strand, within EX28DRAFT_0147 at 92.332 kb on + strand, within EX28DRAFT_0147 at 92.333 kb on - strand, within EX28DRAFT_0147 at 92.618 kb on + strand at 92.633 kb on + strand at 92.634 kb on - strand at 92.676 kb on + strand at 92.677 kb on - strand at 92.828 kb on + strand, within EX28DRAFT_0148 at 92.910 kb on - strand, within EX28DRAFT_0148 at 92.976 kb on + strand, within EX28DRAFT_0148 at 92.977 kb on - strand, within EX28DRAFT_0148 at 93.022 kb on - strand, within EX28DRAFT_0148 at 93.022 kb on - strand, within EX28DRAFT_0148 at 93.137 kb on + strand, within EX28DRAFT_0148 at 93.138 kb on - strand, within EX28DRAFT_0148 at 93.430 kb on + strand, within EX28DRAFT_0148 at 93.430 kb on + strand, within EX28DRAFT_0148 at 93.447 kb on - strand, within EX28DRAFT_0148 at 93.545 kb on + strand, within EX28DRAFT_0148 at 93.546 kb on - strand, within EX28DRAFT_0148 at 93.802 kb on + strand, within EX28DRAFT_0148 at 93.949 kb on + strand, within EX28DRAFT_0148 at 93.984 kb on + strand, within EX28DRAFT_0148 at 93.997 kb on - strand, within EX28DRAFT_0148 at 94.071 kb on + strand, within EX28DRAFT_0148 at 94.072 kb on - strand, within EX28DRAFT_0148 at 94.095 kb on + strand, within EX28DRAFT_0148 at 94.096 kb on - strand, within EX28DRAFT_0148 at 94.096 kb on - strand, within EX28DRAFT_0148 at 94.096 kb on - strand, within EX28DRAFT_0148 at 94.142 kb on + strand, within EX28DRAFT_0148 at 94.142 kb on + strand, within EX28DRAFT_0148 at 94.143 kb on - strand, within EX28DRAFT_0148 at 94.187 kb on + strand, within EX28DRAFT_0148 at 94.188 kb on - strand, within EX28DRAFT_0148 at 94.311 kb on + strand, within EX28DRAFT_0148 at 94.437 kb on + strand at 94.619 kb on - strand at 94.890 kb on - strand at 94.890 kb on - strand at 94.899 kb on + strand at 94.981 kb on + strand, within EX28DRAFT_0149 at 94.982 kb on - strand, within EX28DRAFT_0149 at 94.982 kb on - strand, within EX28DRAFT_0149 at 95.126 kb on + strand, within EX28DRAFT_0149 at 95.166 kb on - strand, within EX28DRAFT_0149 at 95.177 kb on - strand, within EX28DRAFT_0149 at 95.491 kb on + strand, within EX28DRAFT_0149 at 95.491 kb on + strand, within EX28DRAFT_0149
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 91,802 - EX28DRAFT_0147 0.46 +0.6 91,977 + EX28DRAFT_0147 0.58 +0.2 92,113 + EX28DRAFT_0147 0.67 -2.9 92,195 - EX28DRAFT_0147 0.73 -0.3 92,195 - EX28DRAFT_0147 0.73 -1.0 92,245 + EX28DRAFT_0147 0.76 +1.3 92,245 + EX28DRAFT_0147 0.76 +0.1 92,246 - EX28DRAFT_0147 0.76 -2.9 92,246 - EX28DRAFT_0147 0.76 -0.6 92,332 + EX28DRAFT_0147 0.82 +1.6 92,332 + EX28DRAFT_0147 0.82 +0.1 92,332 + EX28DRAFT_0147 0.82 -0.5 92,333 - EX28DRAFT_0147 0.82 -0.5 92,618 + +0.1 92,633 + -0.1 92,634 - -0.9 92,676 + -0.3 92,677 - +0.6 92,828 + EX28DRAFT_0148 0.11 +0.3 92,910 - EX28DRAFT_0148 0.15 +0.6 92,976 + EX28DRAFT_0148 0.19 +0.3 92,977 - EX28DRAFT_0148 0.19 -0.1 93,022 - EX28DRAFT_0148 0.21 -3.1 93,022 - EX28DRAFT_0148 0.21 +0.3 93,137 + EX28DRAFT_0148 0.27 +0.5 93,138 - EX28DRAFT_0148 0.27 -4.2 93,430 + EX28DRAFT_0148 0.42 -0.4 93,430 + EX28DRAFT_0148 0.42 -0.3 93,447 - EX28DRAFT_0148 0.43 -0.1 93,545 + EX28DRAFT_0148 0.48 -0.2 93,546 - EX28DRAFT_0148 0.48 +0.8 93,802 + EX28DRAFT_0148 0.61 -0.8 93,949 + EX28DRAFT_0148 0.69 -0.5 93,984 + EX28DRAFT_0148 0.71 -2.4 93,997 - EX28DRAFT_0148 0.72 +0.1 94,071 + EX28DRAFT_0148 0.75 +1.1 94,072 - EX28DRAFT_0148 0.75 -2.3 94,095 + EX28DRAFT_0148 0.77 -0.1 94,096 - EX28DRAFT_0148 0.77 -0.5 94,096 - EX28DRAFT_0148 0.77 -2.4 94,096 - EX28DRAFT_0148 0.77 -0.6 94,142 + EX28DRAFT_0148 0.79 +0.3 94,142 + EX28DRAFT_0148 0.79 -1.2 94,143 - EX28DRAFT_0148 0.79 +0.3 94,187 + EX28DRAFT_0148 0.81 +0.2 94,188 - EX28DRAFT_0148 0.81 -0.5 94,311 + EX28DRAFT_0148 0.88 -2.3 94,437 + -0.7 94,619 - +0.1 94,890 - -0.4 94,890 - +0.6 94,899 + +0.0 94,981 + EX28DRAFT_0149 0.14 -1.5 94,982 - EX28DRAFT_0149 0.14 +0.7 94,982 - EX28DRAFT_0149 0.14 -0.6 95,126 + EX28DRAFT_0149 0.28 +0.7 95,166 - EX28DRAFT_0149 0.32 +0.6 95,177 - EX28DRAFT_0149 0.34 +0.6 95,491 + EX28DRAFT_0149 0.65 -0.9 95,491 + EX28DRAFT_0149 0.65 +0.2
Or see this region's nucleotide sequence