Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3817 and EX28DRAFT_3818 are separated by 2 nucleotides EX28DRAFT_3818 and EX28DRAFT_3819 overlap by 370 nucleotides EX28DRAFT_3819 and EX28DRAFT_3820 are separated by 246 nucleotides EX28DRAFT_3820 and EX28DRAFT_3821 are separated by 331 nucleotides EX28DRAFT_3821 and EX28DRAFT_3822 are separated by 39 nucleotides
EX28DRAFT_3817: EX28DRAFT_3817 - Lactate dehydrogenase and related dehydrogenases, at 21,895 to 22,869
_3817
EX28DRAFT_3818: EX28DRAFT_3818 - RNA, at 22,872 to 23,297
_3818
EX28DRAFT_3819: EX28DRAFT_3819 - Cold shock proteins, at 22,928 to 23,140
_3819
EX28DRAFT_3820: EX28DRAFT_3820 - Protein of unknown function (DUF3053), at 23,387 to 24,097
_3820
EX28DRAFT_3821: EX28DRAFT_3821 - Predicted transcriptional regulator, at 24,429 to 24,716
_3821
EX28DRAFT_3822: EX28DRAFT_3822 - ATPase components of ABC transporters with duplicated ATPase domains, at 24,756 to 26,375
_3822
Position (kb) 23
24
25 Strain fitness (log2 ratio) -2
-1
0
1
2 at 22.398 kb on + strand, within EX28DRAFT_3817 at 22.838 kb on + strand at 22.839 kb on - strand at 22.868 kb on - strand at 22.929 kb on + strand at 22.930 kb on - strand at 22.938 kb on - strand at 22.946 kb on - strand at 23.046 kb on - strand at 23.140 kb on + strand at 23.141 kb on - strand at 23.141 kb on - strand at 23.152 kb on + strand at 23.162 kb on + strand at 23.162 kb on + strand at 23.165 kb on - strand at 23.205 kb on - strand at 23.205 kb on - strand at 23.236 kb on - strand at 23.239 kb on + strand at 23.240 kb on - strand at 23.264 kb on - strand at 23.276 kb on + strand at 23.277 kb on - strand at 23.303 kb on + strand at 23.307 kb on + strand at 23.307 kb on + strand at 23.338 kb on + strand at 23.389 kb on - strand at 23.470 kb on - strand, within EX28DRAFT_3820 at 23.641 kb on - strand, within EX28DRAFT_3820 at 23.785 kb on + strand, within EX28DRAFT_3820 at 23.785 kb on + strand, within EX28DRAFT_3820 at 23.785 kb on + strand, within EX28DRAFT_3820 at 23.785 kb on + strand, within EX28DRAFT_3820 at 23.786 kb on - strand, within EX28DRAFT_3820 at 23.786 kb on - strand, within EX28DRAFT_3820 at 23.787 kb on + strand, within EX28DRAFT_3820 at 23.787 kb on + strand, within EX28DRAFT_3820 at 23.787 kb on + strand, within EX28DRAFT_3820 at 23.787 kb on + strand, within EX28DRAFT_3820 at 23.788 kb on - strand, within EX28DRAFT_3820 at 23.825 kb on + strand, within EX28DRAFT_3820 at 23.969 kb on - strand, within EX28DRAFT_3820 at 24.100 kb on + strand at 24.101 kb on - strand at 24.185 kb on - strand at 24.185 kb on - strand at 24.259 kb on + strand at 24.259 kb on + strand at 24.260 kb on - strand at 24.260 kb on - strand at 24.266 kb on - strand at 24.278 kb on - strand at 24.282 kb on + strand at 24.283 kb on - strand at 24.286 kb on + strand at 24.286 kb on + strand at 24.286 kb on + strand at 24.287 kb on - strand at 24.307 kb on + strand at 24.359 kb on + strand at 24.360 kb on - strand at 24.369 kb on + strand at 24.369 kb on + strand at 24.414 kb on + strand at 24.435 kb on + strand at 24.435 kb on + strand at 24.438 kb on - strand at 24.438 kb on - strand at 24.494 kb on - strand, within EX28DRAFT_3821 at 24.540 kb on + strand, within EX28DRAFT_3821 at 24.541 kb on - strand, within EX28DRAFT_3821 at 24.541 kb on - strand, within EX28DRAFT_3821 at 24.541 kb on - strand, within EX28DRAFT_3821 at 24.662 kb on - strand, within EX28DRAFT_3821 at 24.662 kb on - strand, within EX28DRAFT_3821 at 24.709 kb on + strand at 24.710 kb on - strand at 24.814 kb on - strand at 24.870 kb on + strand at 24.870 kb on + strand at 24.870 kb on + strand at 24.871 kb on - strand at 24.937 kb on - strand, within EX28DRAFT_3822 at 24.987 kb on - strand, within EX28DRAFT_3822 at 25.091 kb on + strand, within EX28DRAFT_3822 at 25.092 kb on - strand, within EX28DRAFT_3822 at 25.092 kb on - strand, within EX28DRAFT_3822 at 25.092 kb on - strand, within EX28DRAFT_3822
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 22,398 + EX28DRAFT_3817 0.52 +0.0 22,838 + -0.2 22,839 - +1.1 22,868 - +0.4 22,929 + -0.4 22,930 - +0.4 22,938 - +1.0 22,946 - -1.0 23,046 - -0.9 23,140 + +0.8 23,141 - +0.4 23,141 - -0.3 23,152 + +0.0 23,162 + -0.7 23,162 + -0.7 23,165 - +0.5 23,205 - -0.6 23,205 - +0.6 23,236 - +0.1 23,239 + -2.1 23,240 - +0.3 23,264 - +0.2 23,276 + +0.4 23,277 - +0.4 23,303 + +0.4 23,307 + +0.0 23,307 + -1.9 23,338 + +0.1 23,389 - +0.4 23,470 - EX28DRAFT_3820 0.12 -0.0 23,641 - EX28DRAFT_3820 0.36 +0.4 23,785 + EX28DRAFT_3820 0.56 -0.3 23,785 + EX28DRAFT_3820 0.56 -2.0 23,785 + EX28DRAFT_3820 0.56 +0.1 23,785 + EX28DRAFT_3820 0.56 -0.6 23,786 - EX28DRAFT_3820 0.56 +0.3 23,786 - EX28DRAFT_3820 0.56 +0.3 23,787 + EX28DRAFT_3820 0.56 -2.4 23,787 + EX28DRAFT_3820 0.56 -0.3 23,787 + EX28DRAFT_3820 0.56 -2.6 23,787 + EX28DRAFT_3820 0.56 +0.1 23,788 - EX28DRAFT_3820 0.56 -0.5 23,825 + EX28DRAFT_3820 0.62 -0.0 23,969 - EX28DRAFT_3820 0.82 -0.0 24,100 + -0.1 24,101 - -0.4 24,185 - -1.0 24,185 - -0.9 24,259 + +0.1 24,259 + -0.4 24,260 - -0.1 24,260 - +0.3 24,266 - +1.4 24,278 - +0.4 24,282 + -0.1 24,283 - +0.5 24,286 + +0.3 24,286 + +0.4 24,286 + +2.6 24,287 - -0.5 24,307 + +0.2 24,359 + -0.1 24,360 - +0.8 24,369 + +1.2 24,369 + +0.0 24,414 + -0.7 24,435 + -0.5 24,435 + +0.3 24,438 - -0.9 24,438 - -0.1 24,494 - EX28DRAFT_3821 0.23 +0.1 24,540 + EX28DRAFT_3821 0.39 -1.1 24,541 - EX28DRAFT_3821 0.39 +0.3 24,541 - EX28DRAFT_3821 0.39 +0.0 24,541 - EX28DRAFT_3821 0.39 -1.6 24,662 - EX28DRAFT_3821 0.81 +0.4 24,662 - EX28DRAFT_3821 0.81 -0.1 24,709 + +0.7 24,710 - +1.1 24,814 - +1.1 24,870 + +0.6 24,870 + +2.3 24,870 + +0.1 24,871 - +0.0 24,937 - EX28DRAFT_3822 0.11 +0.2 24,987 - EX28DRAFT_3822 0.14 -0.1 25,091 + EX28DRAFT_3822 0.21 -2.0 25,092 - EX28DRAFT_3822 0.21 +0.4 25,092 - EX28DRAFT_3822 0.21 +0.7 25,092 - EX28DRAFT_3822 0.21 +0.4
Or see this region's nucleotide sequence