Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3046 and EX28DRAFT_3047 are separated by 143 nucleotides EX28DRAFT_3047 and EX28DRAFT_3048 are separated by 39 nucleotides EX28DRAFT_3048 and EX28DRAFT_3049 are separated by 195 nucleotides
EX28DRAFT_3046: EX28DRAFT_3046 - cystathionine beta-lyase, bacterial, at 353,058 to 354,245
_3046
EX28DRAFT_3047: EX28DRAFT_3047 - Uncharacterized membrane-associated protein, at 354,389 to 355,048
_3047
EX28DRAFT_3048: EX28DRAFT_3048 - AraC-type DNA-binding domain-containing proteins, at 355,088 to 355,987
_3048
EX28DRAFT_3049: EX28DRAFT_3049 - Uncharacterized oxidoreductases, Fe-dependent alcohol dehydrogenase family, at 356,183 to 357,346
_3049
Position (kb) 355
356 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 354.362 kb on + strand at 354.362 kb on + strand at 354.363 kb on - strand at 354.364 kb on + strand at 354.364 kb on + strand at 354.364 kb on + strand at 354.365 kb on - strand at 354.365 kb on - strand at 354.365 kb on - strand at 354.365 kb on - strand at 354.406 kb on + strand at 354.647 kb on + strand, within EX28DRAFT_3047 at 354.647 kb on + strand, within EX28DRAFT_3047 at 354.716 kb on + strand, within EX28DRAFT_3047 at 354.717 kb on - strand, within EX28DRAFT_3047 at 354.717 kb on - strand, within EX28DRAFT_3047 at 354.917 kb on - strand, within EX28DRAFT_3047 at 355.035 kb on - strand at 355.089 kb on + strand at 355.089 kb on + strand at 355.146 kb on + strand at 355.147 kb on - strand at 355.250 kb on - strand, within EX28DRAFT_3048 at 355.250 kb on - strand, within EX28DRAFT_3048 at 355.268 kb on - strand, within EX28DRAFT_3048 at 355.268 kb on - strand, within EX28DRAFT_3048 at 355.309 kb on + strand, within EX28DRAFT_3048 at 355.597 kb on + strand, within EX28DRAFT_3048 at 355.598 kb on - strand, within EX28DRAFT_3048 at 355.748 kb on + strand, within EX28DRAFT_3048 at 355.764 kb on + strand, within EX28DRAFT_3048 at 355.802 kb on - strand, within EX28DRAFT_3048 at 355.803 kb on + strand, within EX28DRAFT_3048 at 355.988 kb on - strand at 356.195 kb on - strand at 356.288 kb on + strand at 356.510 kb on + strand, within EX28DRAFT_3049 at 356.563 kb on + strand, within EX28DRAFT_3049 at 356.724 kb on - strand, within EX28DRAFT_3049 at 356.776 kb on + strand, within EX28DRAFT_3049 at 356.790 kb on - strand, within EX28DRAFT_3049 at 356.790 kb on - strand, within EX28DRAFT_3049 at 356.799 kb on - strand, within EX28DRAFT_3049 at 356.913 kb on + strand, within EX28DRAFT_3049 at 356.914 kb on - strand, within EX28DRAFT_3049 at 356.914 kb on - strand, within EX28DRAFT_3049 at 356.914 kb on - strand, within EX28DRAFT_3049 at 356.914 kb on - strand, within EX28DRAFT_3049
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 354,362 + -0.5 354,362 + -0.2 354,363 - -0.5 354,364 + -0.2 354,364 + -0.1 354,364 + +0.3 354,365 - +0.5 354,365 - +2.7 354,365 - -0.2 354,365 - -0.4 354,406 + -0.2 354,647 + EX28DRAFT_3047 0.39 +0.6 354,647 + EX28DRAFT_3047 0.39 -0.0 354,716 + EX28DRAFT_3047 0.50 +0.2 354,717 - EX28DRAFT_3047 0.50 -0.9 354,717 - EX28DRAFT_3047 0.50 -0.1 354,917 - EX28DRAFT_3047 0.80 -0.1 355,035 - +0.7 355,089 + +0.2 355,089 + +0.0 355,146 + -0.6 355,147 - -0.1 355,250 - EX28DRAFT_3048 0.18 -0.3 355,250 - EX28DRAFT_3048 0.18 -0.3 355,268 - EX28DRAFT_3048 0.20 -3.0 355,268 - EX28DRAFT_3048 0.20 -0.2 355,309 + EX28DRAFT_3048 0.25 -2.1 355,597 + EX28DRAFT_3048 0.57 -1.0 355,598 - EX28DRAFT_3048 0.57 +0.2 355,748 + EX28DRAFT_3048 0.73 +0.5 355,764 + EX28DRAFT_3048 0.75 +0.1 355,802 - EX28DRAFT_3048 0.79 +0.3 355,803 + EX28DRAFT_3048 0.79 -0.7 355,988 - -0.0 356,195 - +0.3 356,288 + -1.2 356,510 + EX28DRAFT_3049 0.28 -0.5 356,563 + EX28DRAFT_3049 0.33 -0.5 356,724 - EX28DRAFT_3049 0.46 -0.6 356,776 + EX28DRAFT_3049 0.51 -0.1 356,790 - EX28DRAFT_3049 0.52 +0.0 356,790 - EX28DRAFT_3049 0.52 +0.6 356,799 - EX28DRAFT_3049 0.53 +0.0 356,913 + EX28DRAFT_3049 0.63 +0.3 356,914 - EX28DRAFT_3049 0.63 -0.1 356,914 - EX28DRAFT_3049 0.63 +1.3 356,914 - EX28DRAFT_3049 0.63 +0.1 356,914 - EX28DRAFT_3049 0.63 -0.1
Or see this region's nucleotide sequence