Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2651 and EX28DRAFT_2652 are separated by 304 nucleotides EX28DRAFT_2652 and EX28DRAFT_2653 are separated by 109 nucleotides EX28DRAFT_2653 and EX28DRAFT_2654 are separated by 57 nucleotides
EX28DRAFT_2651: EX28DRAFT_2651 - glutamate 5-kinase, at 2,688,359 to 2,689,462
_2651
EX28DRAFT_2652: EX28DRAFT_2652 - Outer membrane protein (porin), at 2,689,767 to 2,690,819
_2652
EX28DRAFT_2653: EX28DRAFT_2653 - Transcriptional regulator Crl, at 2,690,929 to 2,691,330
_2653
EX28DRAFT_2654: EX28DRAFT_2654 - Alpha/beta hydrolase of unknown function (DUF1100), at 2,691,388 to 2,692,632
_2654
Position (kb)
2689
2690
2691 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2689.182 kb on + strand, within EX28DRAFT_2651 at 2689.182 kb on + strand, within EX28DRAFT_2651 at 2689.183 kb on - strand, within EX28DRAFT_2651 at 2689.331 kb on - strand, within EX28DRAFT_2651 at 2689.331 kb on - strand, within EX28DRAFT_2651 at 2689.331 kb on - strand, within EX28DRAFT_2651 at 2689.368 kb on - strand at 2689.368 kb on - strand at 2689.543 kb on + strand at 2689.544 kb on - strand at 2689.649 kb on - strand at 2689.750 kb on - strand at 2689.839 kb on + strand at 2689.839 kb on + strand at 2689.841 kb on + strand at 2689.853 kb on + strand at 2689.857 kb on - strand at 2689.857 kb on - strand at 2689.870 kb on - strand at 2689.893 kb on + strand, within EX28DRAFT_2652 at 2689.893 kb on + strand, within EX28DRAFT_2652 at 2689.894 kb on - strand, within EX28DRAFT_2652 at 2689.894 kb on - strand, within EX28DRAFT_2652 at 2689.932 kb on + strand, within EX28DRAFT_2652 at 2689.933 kb on - strand, within EX28DRAFT_2652 at 2689.933 kb on - strand, within EX28DRAFT_2652 at 2690.016 kb on - strand, within EX28DRAFT_2652 at 2690.144 kb on + strand, within EX28DRAFT_2652 at 2690.145 kb on - strand, within EX28DRAFT_2652 at 2690.277 kb on + strand, within EX28DRAFT_2652 at 2690.278 kb on - strand, within EX28DRAFT_2652 at 2690.346 kb on + strand, within EX28DRAFT_2652 at 2690.346 kb on + strand, within EX28DRAFT_2652 at 2690.347 kb on - strand, within EX28DRAFT_2652 at 2690.473 kb on - strand, within EX28DRAFT_2652 at 2690.490 kb on + strand, within EX28DRAFT_2652 at 2690.505 kb on + strand, within EX28DRAFT_2652 at 2690.523 kb on - strand, within EX28DRAFT_2652 at 2690.583 kb on + strand, within EX28DRAFT_2652 at 2690.676 kb on + strand, within EX28DRAFT_2652 at 2690.676 kb on - strand, within EX28DRAFT_2652 at 2690.802 kb on - strand at 2690.808 kb on + strand at 2690.809 kb on - strand at 2690.869 kb on + strand at 2690.870 kb on - strand at 2690.870 kb on - strand at 2690.927 kb on + strand at 2691.036 kb on + strand, within EX28DRAFT_2653 at 2691.037 kb on - strand, within EX28DRAFT_2653 at 2691.037 kb on - strand, within EX28DRAFT_2653 at 2691.037 kb on - strand, within EX28DRAFT_2653 at 2691.037 kb on - strand, within EX28DRAFT_2653 at 2691.038 kb on + strand, within EX28DRAFT_2653 at 2691.039 kb on - strand, within EX28DRAFT_2653 at 2691.120 kb on - strand, within EX28DRAFT_2653 at 2691.123 kb on + strand, within EX28DRAFT_2653 at 2691.123 kb on + strand, within EX28DRAFT_2653 at 2691.124 kb on - strand, within EX28DRAFT_2653 at 2691.125 kb on + strand, within EX28DRAFT_2653 at 2691.265 kb on - strand, within EX28DRAFT_2653 at 2691.300 kb on + strand at 2691.386 kb on + strand at 2691.387 kb on - strand at 2691.608 kb on + strand, within EX28DRAFT_2654 at 2691.684 kb on - strand, within EX28DRAFT_2654 at 2691.684 kb on - strand, within EX28DRAFT_2654 at 2691.694 kb on + strand, within EX28DRAFT_2654 at 2691.695 kb on - strand, within EX28DRAFT_2654 at 2691.780 kb on + strand, within EX28DRAFT_2654 at 2691.781 kb on - strand, within EX28DRAFT_2654 at 2691.782 kb on + strand, within EX28DRAFT_2654 at 2691.783 kb on - strand, within EX28DRAFT_2654
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 2,689,182 + EX28DRAFT_2651 0.75 -2.2 2,689,182 + EX28DRAFT_2651 0.75 -2.7 2,689,183 - EX28DRAFT_2651 0.75 -2.4 2,689,331 - EX28DRAFT_2651 0.88 -3.4 2,689,331 - EX28DRAFT_2651 0.88 -4.5 2,689,331 - EX28DRAFT_2651 0.88 -3.5 2,689,368 - -2.8 2,689,368 - -3.0 2,689,543 + -0.8 2,689,544 - -2.0 2,689,649 - -0.3 2,689,750 - -1.1 2,689,839 + -0.5 2,689,839 + -1.6 2,689,841 + +0.4 2,689,853 + +0.4 2,689,857 - +0.2 2,689,857 - +0.3 2,689,870 - +0.0 2,689,893 + EX28DRAFT_2652 0.12 +0.0 2,689,893 + EX28DRAFT_2652 0.12 -0.1 2,689,894 - EX28DRAFT_2652 0.12 +0.1 2,689,894 - EX28DRAFT_2652 0.12 -0.4 2,689,932 + EX28DRAFT_2652 0.16 +0.8 2,689,933 - EX28DRAFT_2652 0.16 -0.2 2,689,933 - EX28DRAFT_2652 0.16 +0.5 2,690,016 - EX28DRAFT_2652 0.24 -0.3 2,690,144 + EX28DRAFT_2652 0.36 +0.2 2,690,145 - EX28DRAFT_2652 0.36 +0.4 2,690,277 + EX28DRAFT_2652 0.48 +0.4 2,690,278 - EX28DRAFT_2652 0.49 -0.0 2,690,346 + EX28DRAFT_2652 0.55 +1.9 2,690,346 + EX28DRAFT_2652 0.55 -1.3 2,690,347 - EX28DRAFT_2652 0.55 +0.7 2,690,473 - EX28DRAFT_2652 0.67 +0.2 2,690,490 + EX28DRAFT_2652 0.69 +0.2 2,690,505 + EX28DRAFT_2652 0.70 +0.7 2,690,523 - EX28DRAFT_2652 0.72 -0.0 2,690,583 + EX28DRAFT_2652 0.77 +0.3 2,690,676 + EX28DRAFT_2652 0.86 +0.0 2,690,676 - EX28DRAFT_2652 0.86 -0.5 2,690,802 - -0.2 2,690,808 + +0.1 2,690,809 - -0.8 2,690,869 + -0.4 2,690,870 - +0.0 2,690,870 - -0.5 2,690,927 + +0.9 2,691,036 + EX28DRAFT_2653 0.27 +0.4 2,691,037 - EX28DRAFT_2653 0.27 +0.6 2,691,037 - EX28DRAFT_2653 0.27 +3.0 2,691,037 - EX28DRAFT_2653 0.27 -0.1 2,691,037 - EX28DRAFT_2653 0.27 -2.0 2,691,038 + EX28DRAFT_2653 0.27 -0.4 2,691,039 - EX28DRAFT_2653 0.27 +1.0 2,691,120 - EX28DRAFT_2653 0.48 +1.0 2,691,123 + EX28DRAFT_2653 0.48 +1.2 2,691,123 + EX28DRAFT_2653 0.48 -0.2 2,691,124 - EX28DRAFT_2653 0.49 +0.1 2,691,125 + EX28DRAFT_2653 0.49 +0.0 2,691,265 - EX28DRAFT_2653 0.84 +0.8 2,691,300 + +0.0 2,691,386 + -0.4 2,691,387 - +0.1 2,691,608 + EX28DRAFT_2654 0.18 -0.0 2,691,684 - EX28DRAFT_2654 0.24 +0.2 2,691,684 - EX28DRAFT_2654 0.24 -0.2 2,691,694 + EX28DRAFT_2654 0.25 -0.1 2,691,695 - EX28DRAFT_2654 0.25 +0.8 2,691,780 + EX28DRAFT_2654 0.31 -0.3 2,691,781 - EX28DRAFT_2654 0.32 -0.3 2,691,782 + EX28DRAFT_2654 0.32 -0.5 2,691,783 - EX28DRAFT_2654 0.32 -0.0
Or see this region's nucleotide sequence