Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2314 and EX28DRAFT_2315 are separated by 400 nucleotides EX28DRAFT_2315 and EX28DRAFT_2316 overlap by 5 nucleotides EX28DRAFT_2316 and EX28DRAFT_2317 are separated by 5 nucleotides EX28DRAFT_2317 and EX28DRAFT_2318 overlap by 1 nucleotides
EX28DRAFT_2314: EX28DRAFT_2314 - apolipoprotein N-acyltransferase, at 2,339,130 to 2,340,668
_2314
EX28DRAFT_2315: EX28DRAFT_2315 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 2,341,069 to 2,341,974
_2315
EX28DRAFT_2316: EX28DRAFT_2316 - RNA, at 2,341,970 to 2,342,124
_2316
EX28DRAFT_2317: EX28DRAFT_2317 - amine acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family, at 2,342,130 to 2,342,870
_2317
EX28DRAFT_2318: EX28DRAFT_2318 - amine acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family, at 2,342,870 to 2,343,544
_2318
Position (kb)
2341
2342 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2340.727 kb on - strand at 2340.727 kb on - strand at 2340.850 kb on + strand at 2340.851 kb on - strand at 2340.888 kb on - strand at 2340.963 kb on + strand at 2340.964 kb on - strand at 2340.984 kb on - strand at 2341.010 kb on - strand at 2341.011 kb on + strand at 2341.011 kb on + strand at 2341.011 kb on + strand at 2341.012 kb on - strand at 2341.012 kb on - strand at 2341.012 kb on - strand at 2341.053 kb on - strand at 2341.080 kb on + strand at 2341.231 kb on + strand, within EX28DRAFT_2315 at 2341.271 kb on - strand, within EX28DRAFT_2315 at 2341.271 kb on - strand, within EX28DRAFT_2315 at 2341.359 kb on + strand, within EX28DRAFT_2315 at 2341.452 kb on + strand, within EX28DRAFT_2315 at 2341.672 kb on - strand, within EX28DRAFT_2315 at 2341.773 kb on + strand, within EX28DRAFT_2315 at 2341.972 kb on + strand at 2341.973 kb on - strand at 2341.973 kb on - strand at 2341.976 kb on + strand at 2341.976 kb on + strand at 2341.977 kb on - strand at 2341.977 kb on - strand at 2342.033 kb on + strand, within EX28DRAFT_2316 at 2342.033 kb on + strand, within EX28DRAFT_2316 at 2342.034 kb on - strand, within EX28DRAFT_2316 at 2342.035 kb on + strand, within EX28DRAFT_2316 at 2342.126 kb on + strand at 2342.126 kb on + strand at 2342.127 kb on - strand at 2342.129 kb on + strand at 2342.130 kb on - strand at 2342.138 kb on - strand at 2342.138 kb on - strand at 2342.155 kb on + strand at 2342.155 kb on + strand at 2342.190 kb on + strand at 2342.190 kb on + strand at 2342.190 kb on + strand at 2342.191 kb on - strand at 2342.283 kb on - strand, within EX28DRAFT_2317 at 2342.331 kb on + strand, within EX28DRAFT_2317 at 2342.331 kb on + strand, within EX28DRAFT_2317 at 2342.375 kb on + strand, within EX28DRAFT_2317 at 2342.784 kb on + strand, within EX28DRAFT_2317 at 2342.785 kb on - strand, within EX28DRAFT_2317 at 2342.868 kb on + strand at 2342.868 kb on + strand at 2342.869 kb on - strand at 2342.912 kb on + strand at 2342.913 kb on - strand at 2342.935 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 2,340,727 - -2.4 2,340,727 - +1.1 2,340,850 + +0.3 2,340,851 - +0.3 2,340,888 - -0.8 2,340,963 + +0.3 2,340,964 - +0.2 2,340,984 - +0.2 2,341,010 - +0.0 2,341,011 + -0.6 2,341,011 + +0.4 2,341,011 + -0.2 2,341,012 - +0.5 2,341,012 - -1.6 2,341,012 - -1.6 2,341,053 - +1.5 2,341,080 + +0.3 2,341,231 + EX28DRAFT_2315 0.18 +0.6 2,341,271 - EX28DRAFT_2315 0.22 +0.0 2,341,271 - EX28DRAFT_2315 0.22 +0.1 2,341,359 + EX28DRAFT_2315 0.32 +0.7 2,341,452 + EX28DRAFT_2315 0.42 +0.3 2,341,672 - EX28DRAFT_2315 0.67 -0.7 2,341,773 + EX28DRAFT_2315 0.78 -0.5 2,341,972 + +1.2 2,341,973 - +0.5 2,341,973 - -3.8 2,341,976 + -0.0 2,341,976 + +0.5 2,341,977 - +1.4 2,341,977 - +0.4 2,342,033 + EX28DRAFT_2316 0.41 -1.4 2,342,033 + EX28DRAFT_2316 0.41 -0.4 2,342,034 - EX28DRAFT_2316 0.41 -0.5 2,342,035 + EX28DRAFT_2316 0.42 +0.4 2,342,126 + -0.3 2,342,126 + -0.1 2,342,127 - -0.3 2,342,129 + +0.4 2,342,130 - -2.8 2,342,138 - -0.0 2,342,138 - +1.1 2,342,155 + -0.0 2,342,155 + +0.2 2,342,190 + -0.2 2,342,190 + +0.6 2,342,190 + +1.0 2,342,191 - +0.5 2,342,283 - EX28DRAFT_2317 0.21 -0.5 2,342,331 + EX28DRAFT_2317 0.27 -0.6 2,342,331 + EX28DRAFT_2317 0.27 -0.1 2,342,375 + EX28DRAFT_2317 0.33 -0.5 2,342,784 + EX28DRAFT_2317 0.88 -0.6 2,342,785 - EX28DRAFT_2317 0.88 +0.9 2,342,868 + +0.4 2,342,868 + +0.6 2,342,869 - -0.4 2,342,912 + +0.2 2,342,913 - +1.2 2,342,935 + -0.6
Or see this region's nucleotide sequence