Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0412 and EX28DRAFT_0413 are separated by 129 nucleotides EX28DRAFT_0413 and EX28DRAFT_0414 overlap by 4 nucleotides EX28DRAFT_0414 and EX28DRAFT_0415 are separated by 191 nucleotides EX28DRAFT_0415 and EX28DRAFT_0416 are separated by 59 nucleotides
EX28DRAFT_0412: EX28DRAFT_0412 - cytidine deaminase (EC 3.5.4.5), at 390,407 to 391,291
_0412
EX28DRAFT_0413: EX28DRAFT_0413 - TIGR00659 family protein, at 391,421 to 392,116
_0413
EX28DRAFT_0414: EX28DRAFT_0414 - Putative effector of murein hydrolase LrgA, at 392,113 to 392,508
_0414
EX28DRAFT_0415: EX28DRAFT_0415 - GMP synthase - Glutamine amidotransferase domain, at 392,700 to 393,416
_0415
EX28DRAFT_0416: EX28DRAFT_0416 - AraC-type DNA-binding domain-containing proteins, at 393,476 to 394,273
_0416
Position (kb)
392
393 Strain fitness (log2 ratio)
-1
0
1
2 at 391.306 kb on - strand at 391.507 kb on + strand, within EX28DRAFT_0413 at 391.525 kb on - strand, within EX28DRAFT_0413 at 391.598 kb on - strand, within EX28DRAFT_0413 at 391.845 kb on - strand, within EX28DRAFT_0413 at 391.884 kb on + strand, within EX28DRAFT_0413 at 391.884 kb on + strand, within EX28DRAFT_0413 at 391.886 kb on + strand, within EX28DRAFT_0413 at 391.886 kb on + strand, within EX28DRAFT_0413 at 391.953 kb on + strand, within EX28DRAFT_0413 at 392.104 kb on + strand at 392.142 kb on + strand at 392.255 kb on + strand, within EX28DRAFT_0414 at 392.300 kb on + strand, within EX28DRAFT_0414 at 392.441 kb on + strand, within EX28DRAFT_0414 at 392.442 kb on - strand, within EX28DRAFT_0414 at 392.453 kb on + strand, within EX28DRAFT_0414 at 392.454 kb on - strand, within EX28DRAFT_0414 at 392.454 kb on - strand, within EX28DRAFT_0414 at 392.514 kb on + strand at 392.590 kb on + strand at 392.591 kb on - strand at 392.591 kb on - strand at 392.620 kb on - strand at 392.912 kb on - strand, within EX28DRAFT_0415 at 392.945 kb on - strand, within EX28DRAFT_0415 at 392.981 kb on + strand, within EX28DRAFT_0415 at 392.982 kb on - strand, within EX28DRAFT_0415 at 392.982 kb on - strand, within EX28DRAFT_0415 at 393.175 kb on - strand, within EX28DRAFT_0415 at 393.186 kb on + strand, within EX28DRAFT_0415 at 393.186 kb on + strand, within EX28DRAFT_0415 at 393.328 kb on + strand, within EX28DRAFT_0415 at 393.335 kb on + strand, within EX28DRAFT_0415 at 393.336 kb on - strand, within EX28DRAFT_0415 at 393.338 kb on + strand, within EX28DRAFT_0415 at 393.434 kb on - strand at 393.439 kb on + strand at 393.440 kb on - strand at 393.453 kb on + strand at 393.453 kb on + strand at 393.454 kb on - strand at 393.454 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 391,306 - -0.1 391,507 + EX28DRAFT_0413 0.12 -0.3 391,525 - EX28DRAFT_0413 0.15 +2.3 391,598 - EX28DRAFT_0413 0.25 -0.6 391,845 - EX28DRAFT_0413 0.61 +0.7 391,884 + EX28DRAFT_0413 0.67 +0.2 391,884 + EX28DRAFT_0413 0.67 +0.3 391,886 + EX28DRAFT_0413 0.67 +0.5 391,886 + EX28DRAFT_0413 0.67 -0.4 391,953 + EX28DRAFT_0413 0.76 -1.1 392,104 + -0.7 392,142 + +0.5 392,255 + EX28DRAFT_0414 0.36 -0.1 392,300 + EX28DRAFT_0414 0.47 -0.0 392,441 + EX28DRAFT_0414 0.83 -1.0 392,442 - EX28DRAFT_0414 0.83 +0.9 392,453 + EX28DRAFT_0414 0.86 -0.3 392,454 - EX28DRAFT_0414 0.86 -0.9 392,454 - EX28DRAFT_0414 0.86 -0.0 392,514 + -1.2 392,590 + +0.1 392,591 - -0.3 392,591 - +0.7 392,620 - -0.0 392,912 - EX28DRAFT_0415 0.30 +0.1 392,945 - EX28DRAFT_0415 0.34 +0.7 392,981 + EX28DRAFT_0415 0.39 -1.0 392,982 - EX28DRAFT_0415 0.39 +0.5 392,982 - EX28DRAFT_0415 0.39 +0.9 393,175 - EX28DRAFT_0415 0.66 -1.4 393,186 + EX28DRAFT_0415 0.68 +0.7 393,186 + EX28DRAFT_0415 0.68 -0.1 393,328 + EX28DRAFT_0415 0.88 +0.6 393,335 + EX28DRAFT_0415 0.89 -0.3 393,336 - EX28DRAFT_0415 0.89 -0.8 393,338 + EX28DRAFT_0415 0.89 +0.0 393,434 - +0.4 393,439 + +0.1 393,440 - -1.7 393,453 + +0.1 393,453 + +0.5 393,454 - +0.7 393,454 - +0.0
Or see this region's nucleotide sequence