Strain Fitness in Pseudomonas sp. RS175 around PFR28_00007

Experiment: D-Glucosamine Hydrochloride carbon 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFR28_00006 and PFR28_00007 overlap by 4 nucleotidesPFR28_00007 and PFR28_00008 overlap by 4 nucleotides PFR28_00006: PFR28_00006 - RecBCD enzyme subunit RecC, at 5,884 to 9,336 _00006 PFR28_00007: PFR28_00007 - RecBCD enzyme subunit RecB, at 9,333 to 13,022 _00007 PFR28_00008: PFR28_00008 - RecBCD enzyme subunit RecD, at 13,019 to 15,115 _00008 Position (kb) 9 10 11 12 13 14Strain fitness (log2 ratio) -2 -1 0 1at 8.375 kb on + strand, within PFR28_00006at 8.744 kb on + strand, within PFR28_00006at 8.744 kb on + strand, within PFR28_00006at 12.136 kb on + strand, within PFR28_00007at 12.136 kb on + strand, within PFR28_00007at 12.136 kb on + strand, within PFR28_00007at 12.973 kb on + strandat 13.004 kb on - strandat 13.018 kb on + strandat 13.018 kb on + strandat 13.019 kb on - strandat 13.019 kb on - strandat 13.019 kb on - strandat 13.019 kb on - strandat 13.074 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride carbon 10 mM
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8,375 + PFR28_00006 0.72 -0.0
8,744 + PFR28_00006 0.83 +0.2
8,744 + PFR28_00006 0.83 -1.0
12,136 + PFR28_00007 0.76 +1.0
12,136 + PFR28_00007 0.76 +1.4
12,136 + PFR28_00007 0.76 -0.2
12,973 + -0.0
13,004 - -1.2
13,018 + -0.5
13,018 + -0.7
13,019 - -1.9
13,019 - -0.3
13,019 - -0.2
13,019 - +0.8
13,074 + -0.2

Or see this region's nucleotide sequence