Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4542 and EX28DRAFT_4543 are separated by 11 nucleotides EX28DRAFT_4543 and EX28DRAFT_4544 are separated by 49 nucleotides EX28DRAFT_4544 and EX28DRAFT_4545 are separated by 15 nucleotides
EX28DRAFT_4542: EX28DRAFT_4542 - Bacterial nucleoid DNA-binding protein, at 24,833 to 25,105
_4542
EX28DRAFT_4543: EX28DRAFT_4543 - Protein of unknown function (DUF1481), at 25,117 to 25,809
_4543
EX28DRAFT_4544: EX28DRAFT_4544 - phosphoribosylamine--glycine ligase (EC 6.3.4.13), at 25,859 to 27,151
_4544
EX28DRAFT_4545: EX28DRAFT_4545 - AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful), at 27,167 to 28,756
_4545
Position (kb) 25
26
27
28 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 24.859 kb on - strand at 24.895 kb on + strand, within EX28DRAFT_4542 at 24.913 kb on + strand, within EX28DRAFT_4542 at 25.006 kb on + strand, within EX28DRAFT_4542 at 25.079 kb on - strand at 25.099 kb on + strand at 25.103 kb on + strand at 25.104 kb on - strand at 25.129 kb on - strand at 25.344 kb on + strand, within EX28DRAFT_4543 at 25.344 kb on + strand, within EX28DRAFT_4543 at 25.344 kb on + strand, within EX28DRAFT_4543 at 25.345 kb on - strand, within EX28DRAFT_4543 at 25.345 kb on - strand, within EX28DRAFT_4543 at 25.345 kb on - strand, within EX28DRAFT_4543 at 25.406 kb on - strand, within EX28DRAFT_4543 at 25.406 kb on - strand, within EX28DRAFT_4543 at 25.465 kb on - strand, within EX28DRAFT_4543 at 25.679 kb on + strand, within EX28DRAFT_4543 at 25.679 kb on + strand, within EX28DRAFT_4543 at 25.679 kb on + strand, within EX28DRAFT_4543 at 25.680 kb on - strand, within EX28DRAFT_4543 at 25.680 kb on - strand, within EX28DRAFT_4543 at 25.680 kb on - strand, within EX28DRAFT_4543 at 25.680 kb on - strand, within EX28DRAFT_4543 at 25.680 kb on - strand, within EX28DRAFT_4543 at 25.680 kb on - strand, within EX28DRAFT_4543 at 25.779 kb on + strand at 25.812 kb on + strand at 25.813 kb on - strand at 26.118 kb on + strand, within EX28DRAFT_4544 at 27.169 kb on - strand at 28.041 kb on + strand, within EX28DRAFT_4545 at 28.041 kb on + strand, within EX28DRAFT_4545
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 24,859 - -0.4 24,895 + EX28DRAFT_4542 0.23 +0.5 24,913 + EX28DRAFT_4542 0.29 +0.0 25,006 + EX28DRAFT_4542 0.63 +0.9 25,079 - +1.6 25,099 + +0.5 25,103 + +0.5 25,104 - +0.5 25,129 - +0.1 25,344 + EX28DRAFT_4543 0.33 +1.5 25,344 + EX28DRAFT_4543 0.33 -0.1 25,344 + EX28DRAFT_4543 0.33 -0.8 25,345 - EX28DRAFT_4543 0.33 +1.1 25,345 - EX28DRAFT_4543 0.33 +0.5 25,345 - EX28DRAFT_4543 0.33 -0.1 25,406 - EX28DRAFT_4543 0.42 -0.0 25,406 - EX28DRAFT_4543 0.42 +1.6 25,465 - EX28DRAFT_4543 0.50 +0.1 25,679 + EX28DRAFT_4543 0.81 +1.1 25,679 + EX28DRAFT_4543 0.81 +0.2 25,679 + EX28DRAFT_4543 0.81 -0.1 25,680 - EX28DRAFT_4543 0.81 +1.4 25,680 - EX28DRAFT_4543 0.81 +0.7 25,680 - EX28DRAFT_4543 0.81 -0.5 25,680 - EX28DRAFT_4543 0.81 -0.4 25,680 - EX28DRAFT_4543 0.81 +1.3 25,680 - EX28DRAFT_4543 0.81 +0.6 25,779 + +0.2 25,812 + +1.3 25,813 - -0.1 26,118 + EX28DRAFT_4544 0.20 -3.5 27,169 - -0.2 28,041 + EX28DRAFT_4545 0.55 -1.5 28,041 + EX28DRAFT_4545 0.55 -3.3
Or see this region's nucleotide sequence