Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4536 and EX28DRAFT_4537 are separated by 244 nucleotides EX28DRAFT_4537 and EX28DRAFT_4538 are separated by 95 nucleotides EX28DRAFT_4538 and EX28DRAFT_4539 are separated by 38 nucleotides
EX28DRAFT_4536: EX28DRAFT_4536 - phosphomethylpyrimidine synthase, at 18,724 to 20,619
_4536
EX28DRAFT_4537: EX28DRAFT_4537 - Regulator of sigma D, at 20,864 to 21,361
_4537
EX28DRAFT_4538: EX28DRAFT_4538 - NTP pyrophosphohydrolases containing a Zn-finger, probably nucleic-acid-binding, at 21,457 to 22,230
_4538
EX28DRAFT_4539: EX28DRAFT_4539 - uroporphyrinogen decarboxylase (EC 4.1.1.37), at 22,269 to 23,333
_4539
Position (kb) 20
21
22 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 19.897 kb on - strand, within EX28DRAFT_4536 at 20.012 kb on - strand, within EX28DRAFT_4536 at 20.073 kb on - strand, within EX28DRAFT_4536 at 20.518 kb on - strand at 20.518 kb on - strand at 20.519 kb on + strand at 20.614 kb on - strand at 20.619 kb on + strand at 20.619 kb on + strand at 20.619 kb on + strand at 20.619 kb on + strand at 20.619 kb on + strand at 20.619 kb on + strand at 20.619 kb on + strand at 20.620 kb on - strand at 20.620 kb on - strand at 20.620 kb on - strand at 20.620 kb on - strand at 20.620 kb on - strand at 20.693 kb on - strand at 20.838 kb on + strand at 20.838 kb on + strand at 20.936 kb on - strand, within EX28DRAFT_4537 at 20.936 kb on - strand, within EX28DRAFT_4537 at 21.003 kb on + strand, within EX28DRAFT_4537 at 21.003 kb on + strand, within EX28DRAFT_4537 at 21.003 kb on + strand, within EX28DRAFT_4537 at 21.003 kb on + strand, within EX28DRAFT_4537 at 21.003 kb on + strand, within EX28DRAFT_4537 at 21.003 kb on + strand, within EX28DRAFT_4537 at 21.003 kb on + strand, within EX28DRAFT_4537 at 21.003 kb on + strand, within EX28DRAFT_4537 at 21.004 kb on - strand, within EX28DRAFT_4537 at 21.004 kb on - strand, within EX28DRAFT_4537 at 21.004 kb on - strand, within EX28DRAFT_4537 at 21.004 kb on - strand, within EX28DRAFT_4537 at 21.004 kb on - strand, within EX28DRAFT_4537 at 21.008 kb on - strand, within EX28DRAFT_4537 at 21.042 kb on + strand, within EX28DRAFT_4537 at 21.045 kb on + strand, within EX28DRAFT_4537 at 21.045 kb on + strand, within EX28DRAFT_4537 at 21.045 kb on + strand, within EX28DRAFT_4537 at 21.102 kb on - strand, within EX28DRAFT_4537 at 21.105 kb on + strand, within EX28DRAFT_4537 at 21.145 kb on - strand, within EX28DRAFT_4537 at 21.149 kb on - strand, within EX28DRAFT_4537 at 21.171 kb on + strand, within EX28DRAFT_4537 at 21.172 kb on - strand, within EX28DRAFT_4537 at 21.244 kb on + strand, within EX28DRAFT_4537 at 21.244 kb on + strand, within EX28DRAFT_4537 at 21.245 kb on - strand, within EX28DRAFT_4537 at 21.261 kb on + strand, within EX28DRAFT_4537 at 21.275 kb on + strand, within EX28DRAFT_4537 at 21.276 kb on - strand, within EX28DRAFT_4537 at 21.276 kb on - strand, within EX28DRAFT_4537 at 21.287 kb on + strand, within EX28DRAFT_4537 at 21.288 kb on - strand, within EX28DRAFT_4537 at 21.288 kb on - strand, within EX28DRAFT_4537 at 21.288 kb on - strand, within EX28DRAFT_4537 at 21.293 kb on + strand, within EX28DRAFT_4537 at 21.293 kb on + strand, within EX28DRAFT_4537 at 21.294 kb on - strand, within EX28DRAFT_4537 at 21.357 kb on - strand at 21.357 kb on - strand at 21.380 kb on + strand at 21.381 kb on - strand at 21.407 kb on + strand at 21.444 kb on + strand at 21.452 kb on + strand at 21.453 kb on - strand at 21.453 kb on - strand at 21.453 kb on - strand at 21.453 kb on - strand at 21.456 kb on + strand at 21.456 kb on + strand at 21.456 kb on + strand at 21.456 kb on + strand at 21.457 kb on - strand at 21.465 kb on + strand at 21.466 kb on - strand at 21.466 kb on - strand at 21.469 kb on - strand at 21.519 kb on - strand at 21.684 kb on - strand, within EX28DRAFT_4538 at 21.741 kb on + strand, within EX28DRAFT_4538 at 21.741 kb on + strand, within EX28DRAFT_4538 at 21.742 kb on - strand, within EX28DRAFT_4538 at 21.745 kb on + strand, within EX28DRAFT_4538 at 21.745 kb on + strand, within EX28DRAFT_4538 at 21.745 kb on + strand, within EX28DRAFT_4538 at 21.746 kb on - strand, within EX28DRAFT_4538 at 21.746 kb on - strand, within EX28DRAFT_4538 at 21.746 kb on - strand, within EX28DRAFT_4538 at 21.823 kb on + strand, within EX28DRAFT_4538 at 21.850 kb on - strand, within EX28DRAFT_4538 at 22.069 kb on - strand, within EX28DRAFT_4538 at 22.078 kb on + strand, within EX28DRAFT_4538 at 22.190 kb on + strand at 22.191 kb on - strand at 22.198 kb on - strand at 22.222 kb on + strand at 22.223 kb on - strand at 22.228 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 19,897 - EX28DRAFT_4536 0.62 -0.3 20,012 - EX28DRAFT_4536 0.68 -0.5 20,073 - EX28DRAFT_4536 0.71 +0.5 20,518 - -0.0 20,518 - -0.5 20,519 + -0.2 20,614 - -1.3 20,619 + -1.4 20,619 + +1.2 20,619 + -0.3 20,619 + -0.0 20,619 + +0.7 20,619 + -0.7 20,619 + +3.3 20,620 - -1.3 20,620 - -0.5 20,620 - -0.0 20,620 - -0.5 20,620 - -0.3 20,693 - +0.3 20,838 + -0.5 20,838 + -1.0 20,936 - EX28DRAFT_4537 0.14 +0.4 20,936 - EX28DRAFT_4537 0.14 +0.8 21,003 + EX28DRAFT_4537 0.28 +0.3 21,003 + EX28DRAFT_4537 0.28 +1.8 21,003 + EX28DRAFT_4537 0.28 +0.3 21,003 + EX28DRAFT_4537 0.28 -0.0 21,003 + EX28DRAFT_4537 0.28 +0.2 21,003 + EX28DRAFT_4537 0.28 +1.7 21,003 + EX28DRAFT_4537 0.28 +0.9 21,003 + EX28DRAFT_4537 0.28 +0.3 21,004 - EX28DRAFT_4537 0.28 -0.0 21,004 - EX28DRAFT_4537 0.28 -1.1 21,004 - EX28DRAFT_4537 0.28 -0.2 21,004 - EX28DRAFT_4537 0.28 -0.5 21,004 - EX28DRAFT_4537 0.28 +0.2 21,008 - EX28DRAFT_4537 0.29 +0.1 21,042 + EX28DRAFT_4537 0.36 +0.3 21,045 + EX28DRAFT_4537 0.36 +1.1 21,045 + EX28DRAFT_4537 0.36 +0.6 21,045 + EX28DRAFT_4537 0.36 +0.9 21,102 - EX28DRAFT_4537 0.48 +0.7 21,105 + EX28DRAFT_4537 0.48 +0.5 21,145 - EX28DRAFT_4537 0.56 +0.3 21,149 - EX28DRAFT_4537 0.57 -0.3 21,171 + EX28DRAFT_4537 0.62 -0.1 21,172 - EX28DRAFT_4537 0.62 +0.2 21,244 + EX28DRAFT_4537 0.76 -0.2 21,244 + EX28DRAFT_4537 0.76 +0.4 21,245 - EX28DRAFT_4537 0.77 -0.2 21,261 + EX28DRAFT_4537 0.80 +0.1 21,275 + EX28DRAFT_4537 0.83 -0.1 21,276 - EX28DRAFT_4537 0.83 +0.8 21,276 - EX28DRAFT_4537 0.83 +0.3 21,287 + EX28DRAFT_4537 0.85 +0.2 21,288 - EX28DRAFT_4537 0.85 +0.8 21,288 - EX28DRAFT_4537 0.85 +0.1 21,288 - EX28DRAFT_4537 0.85 +0.3 21,293 + EX28DRAFT_4537 0.86 +0.2 21,293 + EX28DRAFT_4537 0.86 +0.0 21,294 - EX28DRAFT_4537 0.86 -0.8 21,357 - -0.5 21,357 - -1.7 21,380 + -0.1 21,381 - +0.1 21,407 + +0.0 21,444 + +1.0 21,452 + -0.1 21,453 - -0.3 21,453 - -0.4 21,453 - -0.1 21,453 - -1.5 21,456 + +0.4 21,456 + +0.2 21,456 + +0.7 21,456 + +0.4 21,457 - +0.4 21,465 + +0.4 21,466 - +0.4 21,466 - -0.3 21,469 - -0.0 21,519 - -0.5 21,684 - EX28DRAFT_4538 0.29 +0.3 21,741 + EX28DRAFT_4538 0.37 +1.0 21,741 + EX28DRAFT_4538 0.37 +0.2 21,742 - EX28DRAFT_4538 0.37 -0.0 21,745 + EX28DRAFT_4538 0.37 +0.1 21,745 + EX28DRAFT_4538 0.37 -0.4 21,745 + EX28DRAFT_4538 0.37 +0.1 21,746 - EX28DRAFT_4538 0.37 -0.6 21,746 - EX28DRAFT_4538 0.37 -2.0 21,746 - EX28DRAFT_4538 0.37 -0.1 21,823 + EX28DRAFT_4538 0.47 +0.3 21,850 - EX28DRAFT_4538 0.51 +0.4 22,069 - EX28DRAFT_4538 0.79 +0.2 22,078 + EX28DRAFT_4538 0.80 -0.1 22,190 + +0.3 22,191 - +0.4 22,198 - -1.1 22,222 + -0.2 22,223 - +1.0 22,228 + +0.2
Or see this region's nucleotide sequence