Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4531 and EX28DRAFT_4532 overlap by 4 nucleotides EX28DRAFT_4532 and EX28DRAFT_4533 are separated by 1 nucleotides EX28DRAFT_4533 and EX28DRAFT_4534 overlap by 17 nucleotides EX28DRAFT_4534 and EX28DRAFT_4535 overlap by 14 nucleotides
EX28DRAFT_4531: EX28DRAFT_4531 - thiazole biosynthesis protein ThiH, at 15,261 to 16,388
_4531
EX28DRAFT_4532: EX28DRAFT_4532 - Uncharacterized enzyme of thiazole biosynthesis, at 16,385 to 17,155
_4532
EX28DRAFT_4533: EX28DRAFT_4533 - thiamine biosynthesis protein ThiS, at 17,157 to 17,357
_4533
EX28DRAFT_4534: EX28DRAFT_4534 - Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2, at 17,341 to 18,096
_4534
EX28DRAFT_4535: EX28DRAFT_4535 - thiamine-phosphate diphosphorylase, at 18,083 to 18,724
_4535
Position (kb) 16
17
18 Strain fitness (log2 ratio) -1
0
1 at 15.678 kb on - strand, within EX28DRAFT_4531 at 15.678 kb on - strand, within EX28DRAFT_4531 at 15.679 kb on + strand, within EX28DRAFT_4531 at 15.679 kb on + strand, within EX28DRAFT_4531 at 15.680 kb on - strand, within EX28DRAFT_4531 at 15.680 kb on - strand, within EX28DRAFT_4531 at 15.889 kb on + strand, within EX28DRAFT_4531 at 15.889 kb on + strand, within EX28DRAFT_4531 at 15.890 kb on - strand, within EX28DRAFT_4531 at 15.890 kb on - strand, within EX28DRAFT_4531 at 15.970 kb on + strand, within EX28DRAFT_4531 at 16.083 kb on - strand, within EX28DRAFT_4531 at 16.117 kb on + strand, within EX28DRAFT_4531 at 16.117 kb on + strand, within EX28DRAFT_4531 at 16.150 kb on + strand, within EX28DRAFT_4531 at 16.150 kb on + strand, within EX28DRAFT_4531 at 16.163 kb on - strand, within EX28DRAFT_4531 at 16.163 kb on - strand, within EX28DRAFT_4531 at 16.163 kb on - strand, within EX28DRAFT_4531 at 17.015 kb on + strand, within EX28DRAFT_4532 at 17.016 kb on - strand, within EX28DRAFT_4532 at 17.147 kb on - strand at 17.147 kb on - strand at 17.151 kb on - strand at 17.509 kb on + strand, within EX28DRAFT_4534 at 17.671 kb on - strand, within EX28DRAFT_4534
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 15,678 - EX28DRAFT_4531 0.37 -0.4 15,678 - EX28DRAFT_4531 0.37 +0.2 15,679 + EX28DRAFT_4531 0.37 -1.1 15,679 + EX28DRAFT_4531 0.37 -0.6 15,680 - EX28DRAFT_4531 0.37 -0.5 15,680 - EX28DRAFT_4531 0.37 -1.7 15,889 + EX28DRAFT_4531 0.56 -1.3 15,889 + EX28DRAFT_4531 0.56 -0.9 15,890 - EX28DRAFT_4531 0.56 -1.4 15,890 - EX28DRAFT_4531 0.56 -0.4 15,970 + EX28DRAFT_4531 0.63 -1.2 16,083 - EX28DRAFT_4531 0.73 -0.8 16,117 + EX28DRAFT_4531 0.76 +0.9 16,117 + EX28DRAFT_4531 0.76 -0.2 16,150 + EX28DRAFT_4531 0.79 +0.2 16,150 + EX28DRAFT_4531 0.79 -0.1 16,163 - EX28DRAFT_4531 0.80 -1.0 16,163 - EX28DRAFT_4531 0.80 -1.4 16,163 - EX28DRAFT_4531 0.80 -0.0 17,015 + EX28DRAFT_4532 0.82 -0.3 17,016 - EX28DRAFT_4532 0.82 -0.5 17,147 - -0.8 17,147 - -0.7 17,151 - +0.0 17,509 + EX28DRAFT_4534 0.22 -0.8 17,671 - EX28DRAFT_4534 0.44 -0.7
Or see this region's nucleotide sequence