Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4527 and EX28DRAFT_4528 are separated by 114 nucleotides EX28DRAFT_4528 and EX28DRAFT_4529 overlap by 1 nucleotides EX28DRAFT_4529 and EX28DRAFT_4530 are separated by 180 nucleotides
EX28DRAFT_4527: EX28DRAFT_4527 - DNA-directed RNA polymerase, beta' subunit, predominant form, at 8,239 to 12,462
_4527
EX28DRAFT_4528: EX28DRAFT_4528 - Phosphotransferase system cellobiose-specific component IIA, at 12,577 to 12,894
_4528
EX28DRAFT_4529: EX28DRAFT_4529 - Phosphotransferase system cellobiose-specific component IIB, at 12,894 to 13,202
_4529
EX28DRAFT_4530: EX28DRAFT_4530 - FOG: GGDEF domain, at 13,383 to 14,924
_4530
Position (kb) 12
13
14 Strain fitness (log2 ratio) -2
-1
0
1 at 12.345 kb on + strand at 12.562 kb on + strand at 12.562 kb on + strand at 12.570 kb on + strand at 12.570 kb on + strand at 12.571 kb on - strand at 12.571 kb on - strand at 12.571 kb on - strand at 12.896 kb on - strand at 12.928 kb on - strand, within EX28DRAFT_4529 at 13.024 kb on + strand, within EX28DRAFT_4529 at 13.024 kb on + strand, within EX28DRAFT_4529 at 13.024 kb on + strand, within EX28DRAFT_4529 at 13.024 kb on + strand, within EX28DRAFT_4529 at 13.048 kb on - strand, within EX28DRAFT_4529 at 13.143 kb on - strand, within EX28DRAFT_4529 at 13.217 kb on + strand at 13.436 kb on - strand at 13.511 kb on + strand at 13.703 kb on + strand, within EX28DRAFT_4530 at 13.749 kb on - strand, within EX28DRAFT_4530 at 13.805 kb on + strand, within EX28DRAFT_4530 at 13.870 kb on + strand, within EX28DRAFT_4530 at 13.871 kb on - strand, within EX28DRAFT_4530 at 13.902 kb on - strand, within EX28DRAFT_4530 at 13.904 kb on + strand, within EX28DRAFT_4530 at 13.967 kb on + strand, within EX28DRAFT_4530 at 13.967 kb on + strand, within EX28DRAFT_4530 at 14.005 kb on + strand, within EX28DRAFT_4530 at 14.005 kb on + strand, within EX28DRAFT_4530 at 14.135 kb on + strand, within EX28DRAFT_4530 at 14.143 kb on + strand, within EX28DRAFT_4530 at 14.144 kb on - strand, within EX28DRAFT_4530 at 14.146 kb on + strand, within EX28DRAFT_4530 at 14.146 kb on + strand, within EX28DRAFT_4530 at 14.146 kb on + strand, within EX28DRAFT_4530 at 14.147 kb on - strand, within EX28DRAFT_4530 at 14.147 kb on - strand, within EX28DRAFT_4530 at 14.149 kb on + strand, within EX28DRAFT_4530 at 14.149 kb on + strand, within EX28DRAFT_4530 at 14.150 kb on - strand, within EX28DRAFT_4530 at 14.152 kb on + strand, within EX28DRAFT_4530 at 14.176 kb on + strand, within EX28DRAFT_4530 at 14.177 kb on - strand, within EX28DRAFT_4530
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 12,345 + +0.5 12,562 + -0.0 12,562 + +0.9 12,570 + +0.4 12,570 + -0.3 12,571 - -0.0 12,571 - +0.2 12,571 - -0.4 12,896 - -0.4 12,928 - EX28DRAFT_4529 0.11 +0.1 13,024 + EX28DRAFT_4529 0.42 +0.1 13,024 + EX28DRAFT_4529 0.42 +0.2 13,024 + EX28DRAFT_4529 0.42 +0.1 13,024 + EX28DRAFT_4529 0.42 +0.2 13,048 - EX28DRAFT_4529 0.50 +0.7 13,143 - EX28DRAFT_4529 0.81 +0.4 13,217 + -0.1 13,436 - +0.4 13,511 + +0.6 13,703 + EX28DRAFT_4530 0.21 -1.3 13,749 - EX28DRAFT_4530 0.24 +1.2 13,805 + EX28DRAFT_4530 0.27 +0.4 13,870 + EX28DRAFT_4530 0.32 +0.3 13,871 - EX28DRAFT_4530 0.32 +0.4 13,902 - EX28DRAFT_4530 0.34 -0.1 13,904 + EX28DRAFT_4530 0.34 +0.0 13,967 + EX28DRAFT_4530 0.38 -0.0 13,967 + EX28DRAFT_4530 0.38 -0.4 14,005 + EX28DRAFT_4530 0.40 -0.2 14,005 + EX28DRAFT_4530 0.40 +1.0 14,135 + EX28DRAFT_4530 0.49 +0.4 14,143 + EX28DRAFT_4530 0.49 +1.2 14,144 - EX28DRAFT_4530 0.49 +0.1 14,146 + EX28DRAFT_4530 0.49 +0.1 14,146 + EX28DRAFT_4530 0.49 -0.9 14,146 + EX28DRAFT_4530 0.49 -1.5 14,147 - EX28DRAFT_4530 0.50 -0.2 14,147 - EX28DRAFT_4530 0.50 -1.1 14,149 + EX28DRAFT_4530 0.50 -0.3 14,149 + EX28DRAFT_4530 0.50 +0.0 14,150 - EX28DRAFT_4530 0.50 +1.4 14,152 + EX28DRAFT_4530 0.50 +0.5 14,176 + EX28DRAFT_4530 0.51 +0.2 14,177 - EX28DRAFT_4530 0.51 -1.8
Or see this region's nucleotide sequence