Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4490 and EX28DRAFT_4491 are separated by 31 nucleotides EX28DRAFT_4491 and EX28DRAFT_4492 are separated by 82 nucleotides EX28DRAFT_4492 and EX28DRAFT_4493 overlap by 113 nucleotides EX28DRAFT_4493 and EX28DRAFT_4494 are separated by 115 nucleotides
EX28DRAFT_4490: EX28DRAFT_4490 - Lysophospholipase, at 56,011 to 57,003
_4490
EX28DRAFT_4491: EX28DRAFT_4491 - HAD-superfamily hydrolase, subfamily IIB, at 57,035 to 57,835
_4491
EX28DRAFT_4492: EX28DRAFT_4492 - Carboxylate/Amino Acid/Amine Transporter, at 57,918 to 58,817
_4492
EX28DRAFT_4493: EX28DRAFT_4493 - Transcriptional regulator, at 58,705 to 59,658
_4493
EX28DRAFT_4494: EX28DRAFT_4494 - methionine synthase (B12-independent) (EC 2.1.1.14), at 59,774 to 62,035
_4494
Position (kb) 57
58
59 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 56.949 kb on + strand at 56.997 kb on + strand at 56.997 kb on + strand at 56.997 kb on + strand at 57.034 kb on + strand at 57.038 kb on + strand at 57.039 kb on - strand at 57.117 kb on + strand, within EX28DRAFT_4491 at 57.216 kb on - strand, within EX28DRAFT_4491 at 57.216 kb on - strand, within EX28DRAFT_4491 at 57.323 kb on - strand, within EX28DRAFT_4491 at 57.370 kb on + strand, within EX28DRAFT_4491 at 57.371 kb on - strand, within EX28DRAFT_4491 at 57.421 kb on + strand, within EX28DRAFT_4491 at 57.422 kb on - strand, within EX28DRAFT_4491 at 57.422 kb on - strand, within EX28DRAFT_4491 at 57.422 kb on - strand, within EX28DRAFT_4491 at 57.422 kb on - strand, within EX28DRAFT_4491 at 57.422 kb on - strand, within EX28DRAFT_4491 at 57.422 kb on - strand, within EX28DRAFT_4491 at 57.422 kb on - strand, within EX28DRAFT_4491 at 57.426 kb on - strand, within EX28DRAFT_4491 at 57.784 kb on + strand at 57.809 kb on + strand at 57.809 kb on + strand at 57.809 kb on + strand at 57.817 kb on + strand at 57.834 kb on - strand at 57.834 kb on - strand at 57.882 kb on + strand at 57.925 kb on + strand at 57.941 kb on + strand at 58.095 kb on + strand, within EX28DRAFT_4492 at 58.096 kb on + strand, within EX28DRAFT_4492 at 58.096 kb on - strand, within EX28DRAFT_4492 at 58.097 kb on + strand, within EX28DRAFT_4492 at 58.097 kb on + strand, within EX28DRAFT_4492 at 58.098 kb on - strand, within EX28DRAFT_4492 at 58.098 kb on - strand, within EX28DRAFT_4492 at 58.098 kb on - strand, within EX28DRAFT_4492 at 58.131 kb on + strand, within EX28DRAFT_4492 at 58.131 kb on + strand, within EX28DRAFT_4492 at 58.131 kb on + strand, within EX28DRAFT_4492 at 58.131 kb on + strand, within EX28DRAFT_4492 at 58.132 kb on - strand, within EX28DRAFT_4492 at 58.206 kb on + strand, within EX28DRAFT_4492 at 58.305 kb on + strand, within EX28DRAFT_4492 at 58.389 kb on + strand, within EX28DRAFT_4492 at 58.428 kb on - strand, within EX28DRAFT_4492 at 58.446 kb on + strand, within EX28DRAFT_4492 at 58.446 kb on + strand, within EX28DRAFT_4492 at 58.446 kb on + strand, within EX28DRAFT_4492 at 58.447 kb on - strand, within EX28DRAFT_4492 at 58.447 kb on - strand, within EX28DRAFT_4492 at 58.703 kb on + strand, within EX28DRAFT_4492 at 58.704 kb on - strand, within EX28DRAFT_4492 at 58.865 kb on + strand, within EX28DRAFT_4493 at 58.866 kb on - strand, within EX28DRAFT_4493 at 58.866 kb on - strand, within EX28DRAFT_4493 at 59.177 kb on + strand, within EX28DRAFT_4493 at 59.358 kb on + strand, within EX28DRAFT_4493 at 59.387 kb on - strand, within EX28DRAFT_4493 at 59.387 kb on - strand, within EX28DRAFT_4493 at 59.387 kb on - strand, within EX28DRAFT_4493 at 59.506 kb on - strand, within EX28DRAFT_4493 at 59.685 kb on + strand at 59.701 kb on + strand at 59.701 kb on + strand at 59.702 kb on - strand at 59.702 kb on - strand at 59.702 kb on - strand at 59.702 kb on - strand at 59.703 kb on + strand at 59.703 kb on + strand at 59.704 kb on - strand at 59.704 kb on - strand at 59.708 kb on + strand at 59.722 kb on - strand at 59.722 kb on - strand at 59.770 kb on + strand at 59.770 kb on + strand at 59.770 kb on + strand at 59.771 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 56,949 + +0.4 56,997 + +0.1 56,997 + +0.7 56,997 + +1.3 57,034 + +0.6 57,038 + +1.0 57,039 - +0.3 57,117 + EX28DRAFT_4491 0.10 -0.5 57,216 - EX28DRAFT_4491 0.23 +1.3 57,216 - EX28DRAFT_4491 0.23 +0.3 57,323 - EX28DRAFT_4491 0.36 +0.3 57,370 + EX28DRAFT_4491 0.42 +1.6 57,371 - EX28DRAFT_4491 0.42 -0.2 57,421 + EX28DRAFT_4491 0.48 +0.6 57,422 - EX28DRAFT_4491 0.48 +1.4 57,422 - EX28DRAFT_4491 0.48 +0.3 57,422 - EX28DRAFT_4491 0.48 +0.1 57,422 - EX28DRAFT_4491 0.48 +1.3 57,422 - EX28DRAFT_4491 0.48 +0.4 57,422 - EX28DRAFT_4491 0.48 -0.4 57,422 - EX28DRAFT_4491 0.48 +0.0 57,426 - EX28DRAFT_4491 0.49 +1.2 57,784 + +1.0 57,809 + +0.9 57,809 + -1.0 57,809 + +0.1 57,817 + +0.2 57,834 - +0.6 57,834 - +1.2 57,882 + -0.1 57,925 + +1.5 57,941 + -0.8 58,095 + EX28DRAFT_4492 0.20 +0.4 58,096 + EX28DRAFT_4492 0.20 +1.2 58,096 - EX28DRAFT_4492 0.20 -0.9 58,097 + EX28DRAFT_4492 0.20 +0.4 58,097 + EX28DRAFT_4492 0.20 +0.8 58,098 - EX28DRAFT_4492 0.20 +0.7 58,098 - EX28DRAFT_4492 0.20 -1.3 58,098 - EX28DRAFT_4492 0.20 +0.1 58,131 + EX28DRAFT_4492 0.24 -0.2 58,131 + EX28DRAFT_4492 0.24 +1.2 58,131 + EX28DRAFT_4492 0.24 +0.6 58,131 + EX28DRAFT_4492 0.24 +0.4 58,132 - EX28DRAFT_4492 0.24 +0.1 58,206 + EX28DRAFT_4492 0.32 -0.7 58,305 + EX28DRAFT_4492 0.43 -0.0 58,389 + EX28DRAFT_4492 0.52 +1.4 58,428 - EX28DRAFT_4492 0.57 +0.4 58,446 + EX28DRAFT_4492 0.59 +0.1 58,446 + EX28DRAFT_4492 0.59 -0.2 58,446 + EX28DRAFT_4492 0.59 +0.6 58,447 - EX28DRAFT_4492 0.59 -0.5 58,447 - EX28DRAFT_4492 0.59 +0.5 58,703 + EX28DRAFT_4492 0.87 +1.0 58,704 - EX28DRAFT_4492 0.87 -0.4 58,865 + EX28DRAFT_4493 0.17 -4.9 58,866 - EX28DRAFT_4493 0.17 -5.0 58,866 - EX28DRAFT_4493 0.17 -2.2 59,177 + EX28DRAFT_4493 0.49 -5.7 59,358 + EX28DRAFT_4493 0.68 -3.1 59,387 - EX28DRAFT_4493 0.71 -2.7 59,387 - EX28DRAFT_4493 0.71 -3.0 59,387 - EX28DRAFT_4493 0.71 -2.3 59,506 - EX28DRAFT_4493 0.84 -2.6 59,685 + -3.5 59,701 + -3.2 59,701 + -2.1 59,702 - -2.0 59,702 - -1.4 59,702 - +0.9 59,702 - +0.7 59,703 + -1.0 59,703 + -0.4 59,704 - +0.1 59,704 - +0.6 59,708 + -2.5 59,722 - -0.0 59,722 - +0.7 59,770 + -0.1 59,770 + -1.0 59,770 + +0.4 59,771 - -0.3
Or see this region's nucleotide sequence