Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4444 and EX28DRAFT_4445 are separated by 45 nucleotides EX28DRAFT_4445 and EX28DRAFT_4446 are separated by 152 nucleotides
EX28DRAFT_4444: EX28DRAFT_4444 - threonine ammonia-lyase, biosynthetic, long form, at 8,675 to 10,222
_4444
EX28DRAFT_4445: EX28DRAFT_4445 - Transcriptional regulator, at 10,268 to 11,158
_4445
EX28DRAFT_4446: EX28DRAFT_4446 - ketol-acid reductoisomerase (EC 1.1.1.86), at 11,311 to 12,786
_4446
Position (kb)
10
11
12 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 9.299 kb on - strand, within EX28DRAFT_4444 at 9.449 kb on + strand, within EX28DRAFT_4444 at 9.475 kb on + strand, within EX28DRAFT_4444 at 9.485 kb on - strand, within EX28DRAFT_4444 at 9.778 kb on + strand, within EX28DRAFT_4444 at 9.779 kb on - strand, within EX28DRAFT_4444 at 9.811 kb on + strand, within EX28DRAFT_4444 at 9.939 kb on - strand, within EX28DRAFT_4444 at 10.054 kb on + strand, within EX28DRAFT_4444 at 10.055 kb on - strand, within EX28DRAFT_4444 at 10.166 kb on + strand at 10.238 kb on + strand at 10.303 kb on + strand at 10.501 kb on - strand, within EX28DRAFT_4445 at 10.579 kb on + strand, within EX28DRAFT_4445 at 10.580 kb on - strand, within EX28DRAFT_4445 at 10.849 kb on + strand, within EX28DRAFT_4445 at 10.851 kb on + strand, within EX28DRAFT_4445 at 11.150 kb on + strand at 11.151 kb on - strand at 11.242 kb on + strand at 11.252 kb on - strand at 11.281 kb on + strand at 11.281 kb on + strand at 11.281 kb on + strand at 11.282 kb on - strand at 11.303 kb on + strand at 11.303 kb on + strand at 11.303 kb on + strand at 11.320 kb on + strand at 11.320 kb on + strand at 11.321 kb on - strand at 11.321 kb on - strand at 11.325 kb on + strand at 11.328 kb on + strand at 11.329 kb on - strand at 11.584 kb on + strand, within EX28DRAFT_4446 at 11.585 kb on - strand, within EX28DRAFT_4446 at 11.585 kb on - strand, within EX28DRAFT_4446 at 11.663 kb on + strand, within EX28DRAFT_4446 at 11.793 kb on - strand, within EX28DRAFT_4446 at 11.804 kb on - strand, within EX28DRAFT_4446 at 11.804 kb on - strand, within EX28DRAFT_4446 at 12.120 kb on + strand, within EX28DRAFT_4446 at 12.120 kb on + strand, within EX28DRAFT_4446 at 12.120 kb on + strand, within EX28DRAFT_4446 at 12.121 kb on - strand, within EX28DRAFT_4446 at 12.121 kb on - strand, within EX28DRAFT_4446 at 12.121 kb on - strand, within EX28DRAFT_4446
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 9,299 - EX28DRAFT_4444 0.40 -4.7 9,449 + EX28DRAFT_4444 0.50 -2.0 9,475 + EX28DRAFT_4444 0.52 -1.0 9,485 - EX28DRAFT_4444 0.52 -3.0 9,778 + EX28DRAFT_4444 0.71 -3.2 9,779 - EX28DRAFT_4444 0.71 -1.6 9,811 + EX28DRAFT_4444 0.73 -4.4 9,939 - EX28DRAFT_4444 0.82 -3.0 10,054 + EX28DRAFT_4444 0.89 -4.8 10,055 - EX28DRAFT_4444 0.89 -4.7 10,166 + -2.3 10,238 + +0.2 10,303 + -2.6 10,501 - EX28DRAFT_4445 0.26 -1.7 10,579 + EX28DRAFT_4445 0.35 -3.0 10,580 - EX28DRAFT_4445 0.35 -2.4 10,849 + EX28DRAFT_4445 0.65 -2.7 10,851 + EX28DRAFT_4445 0.65 -0.8 11,150 + -1.7 11,151 - -2.1 11,242 + -1.7 11,252 - -5.7 11,281 + -1.6 11,281 + -2.1 11,281 + -1.6 11,282 - -2.4 11,303 + -4.4 11,303 + -2.0 11,303 + -3.7 11,320 + -4.6 11,320 + -4.2 11,321 - -1.4 11,321 - -0.9 11,325 + -2.7 11,328 + -4.9 11,329 - -2.0 11,584 + EX28DRAFT_4446 0.18 -3.8 11,585 - EX28DRAFT_4446 0.19 -4.4 11,585 - EX28DRAFT_4446 0.19 -4.3 11,663 + EX28DRAFT_4446 0.24 -1.2 11,793 - EX28DRAFT_4446 0.33 -4.8 11,804 - EX28DRAFT_4446 0.33 -2.4 11,804 - EX28DRAFT_4446 0.33 -6.7 12,120 + EX28DRAFT_4446 0.55 -5.7 12,120 + EX28DRAFT_4446 0.55 -3.4 12,120 + EX28DRAFT_4446 0.55 -2.6 12,121 - EX28DRAFT_4446 0.55 -5.6 12,121 - EX28DRAFT_4446 0.55 -2.7 12,121 - EX28DRAFT_4446 0.55 -4.2
Or see this region's nucleotide sequence