Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4438 and EX28DRAFT_4439 are separated by 65 nucleotides EX28DRAFT_4439 and EX28DRAFT_4440 are separated by 385 nucleotides EX28DRAFT_4440 and EX28DRAFT_4441 overlap by 4 nucleotides EX28DRAFT_4441 and EX28DRAFT_4442 are separated by 18 nucleotides
EX28DRAFT_4438: EX28DRAFT_4438 - Mg chelatase-related protein, at 1,811 to 3,331
_4438
EX28DRAFT_4439: EX28DRAFT_4439 - hypothetical protein, at 3,397 to 3,525
_4439
EX28DRAFT_4440: EX28DRAFT_4440 - acetolactate synthase, large subunit (EC 2.2.1.6), at 3,911 to 5,557
_4440
EX28DRAFT_4441: EX28DRAFT_4441 - acetolactate synthase, small subunit (EC 2.2.1.6), at 5,554 to 5,817
_4441
EX28DRAFT_4442: EX28DRAFT_4442 - branched-chain amino acid aminotransferase, group I, at 5,836 to 6,765
_4442
Position (kb)
3
4
5
6 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2.943 kb on - strand, within EX28DRAFT_4438 at 3.041 kb on - strand, within EX28DRAFT_4438 at 3.084 kb on - strand, within EX28DRAFT_4438 at 3.158 kb on - strand, within EX28DRAFT_4438 at 3.158 kb on - strand, within EX28DRAFT_4438 at 3.159 kb on + strand, within EX28DRAFT_4438 at 3.166 kb on + strand, within EX28DRAFT_4438 at 3.170 kb on + strand, within EX28DRAFT_4438 at 3.170 kb on + strand, within EX28DRAFT_4438 at 3.171 kb on - strand, within EX28DRAFT_4438 at 3.171 kb on - strand, within EX28DRAFT_4438 at 3.171 kb on - strand, within EX28DRAFT_4438 at 3.253 kb on + strand at 3.258 kb on - strand at 3.258 kb on - strand at 3.492 kb on + strand, within EX28DRAFT_4439 at 3.541 kb on + strand at 3.593 kb on + strand at 3.593 kb on + strand at 3.594 kb on - strand at 3.639 kb on + strand at 3.687 kb on - strand at 3.730 kb on - strand at 3.826 kb on + strand at 3.931 kb on - strand at 3.933 kb on + strand at 3.933 kb on + strand at 3.934 kb on - strand at 3.934 kb on - strand at 3.991 kb on + strand at 4.002 kb on - strand at 4.014 kb on - strand at 4.079 kb on + strand, within EX28DRAFT_4440 at 4.080 kb on - strand, within EX28DRAFT_4440 at 4.390 kb on + strand, within EX28DRAFT_4440 at 4.391 kb on - strand, within EX28DRAFT_4440 at 4.640 kb on + strand, within EX28DRAFT_4440 at 4.641 kb on - strand, within EX28DRAFT_4440 at 4.641 kb on - strand, within EX28DRAFT_4440 at 4.641 kb on - strand, within EX28DRAFT_4440 at 4.786 kb on + strand, within EX28DRAFT_4440 at 4.787 kb on - strand, within EX28DRAFT_4440 at 4.787 kb on - strand, within EX28DRAFT_4440 at 4.967 kb on + strand, within EX28DRAFT_4440 at 4.968 kb on - strand, within EX28DRAFT_4440 at 5.002 kb on + strand, within EX28DRAFT_4440 at 5.003 kb on - strand, within EX28DRAFT_4440 at 5.003 kb on - strand, within EX28DRAFT_4440 at 5.035 kb on - strand, within EX28DRAFT_4440 at 5.072 kb on + strand, within EX28DRAFT_4440 at 5.073 kb on - strand, within EX28DRAFT_4440 at 5.183 kb on - strand, within EX28DRAFT_4440 at 5.550 kb on + strand at 5.550 kb on + strand at 5.652 kb on + strand, within EX28DRAFT_4441 at 5.653 kb on - strand, within EX28DRAFT_4441 at 5.700 kb on - strand, within EX28DRAFT_4441 at 5.730 kb on - strand, within EX28DRAFT_4441 at 5.736 kb on + strand, within EX28DRAFT_4441 at 5.757 kb on - strand, within EX28DRAFT_4441 at 5.857 kb on + strand at 5.858 kb on - strand at 5.929 kb on + strand, within EX28DRAFT_4442 at 5.929 kb on + strand, within EX28DRAFT_4442 at 5.930 kb on - strand, within EX28DRAFT_4442 at 5.935 kb on - strand, within EX28DRAFT_4442 at 5.942 kb on + strand, within EX28DRAFT_4442 at 5.943 kb on - strand, within EX28DRAFT_4442 at 5.943 kb on - strand, within EX28DRAFT_4442 at 5.962 kb on + strand, within EX28DRAFT_4442 at 5.963 kb on - strand, within EX28DRAFT_4442 at 6.182 kb on - strand, within EX28DRAFT_4442 at 6.184 kb on - strand, within EX28DRAFT_4442 at 6.329 kb on - strand, within EX28DRAFT_4442 at 6.407 kb on - strand, within EX28DRAFT_4442 at 6.407 kb on - strand, within EX28DRAFT_4442 at 6.407 kb on - strand, within EX28DRAFT_4442 at 6.453 kb on + strand, within EX28DRAFT_4442 at 6.546 kb on - strand, within EX28DRAFT_4442 at 6.546 kb on - strand, within EX28DRAFT_4442
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 2,943 - EX28DRAFT_4438 0.74 -0.1 3,041 - EX28DRAFT_4438 0.81 -2.0 3,084 - EX28DRAFT_4438 0.84 -0.5 3,158 - EX28DRAFT_4438 0.89 -0.0 3,158 - EX28DRAFT_4438 0.89 -0.4 3,159 + EX28DRAFT_4438 0.89 +0.7 3,166 + EX28DRAFT_4438 0.89 +0.4 3,170 + EX28DRAFT_4438 0.89 +0.3 3,170 + EX28DRAFT_4438 0.89 +0.6 3,171 - EX28DRAFT_4438 0.89 +0.1 3,171 - EX28DRAFT_4438 0.89 +0.4 3,171 - EX28DRAFT_4438 0.89 -1.1 3,253 + -0.6 3,258 - -0.1 3,258 - -0.1 3,492 + EX28DRAFT_4439 0.74 +0.4 3,541 + +0.7 3,593 + -0.9 3,593 + +0.1 3,594 - +0.2 3,639 + +0.3 3,687 - -2.4 3,730 - -1.9 3,826 + +0.6 3,931 - -1.4 3,933 + -0.4 3,933 + -1.0 3,934 - -2.4 3,934 - -1.5 3,991 + -0.2 4,002 - -3.9 4,014 - -4.5 4,079 + EX28DRAFT_4440 0.10 -0.5 4,080 - EX28DRAFT_4440 0.10 -1.7 4,390 + EX28DRAFT_4440 0.29 -1.3 4,391 - EX28DRAFT_4440 0.29 -4.0 4,640 + EX28DRAFT_4440 0.44 -0.5 4,641 - EX28DRAFT_4440 0.44 -3.5 4,641 - EX28DRAFT_4440 0.44 -2.0 4,641 - EX28DRAFT_4440 0.44 -0.8 4,786 + EX28DRAFT_4440 0.53 -0.2 4,787 - EX28DRAFT_4440 0.53 -1.2 4,787 - EX28DRAFT_4440 0.53 -0.7 4,967 + EX28DRAFT_4440 0.64 +1.5 4,968 - EX28DRAFT_4440 0.64 -0.9 5,002 + EX28DRAFT_4440 0.66 -0.4 5,003 - EX28DRAFT_4440 0.66 -3.3 5,003 - EX28DRAFT_4440 0.66 -0.4 5,035 - EX28DRAFT_4440 0.68 -1.9 5,072 + EX28DRAFT_4440 0.70 -1.0 5,073 - EX28DRAFT_4440 0.71 -2.0 5,183 - EX28DRAFT_4440 0.77 -1.4 5,550 + +0.2 5,550 + -0.1 5,652 + EX28DRAFT_4441 0.37 -0.1 5,653 - EX28DRAFT_4441 0.38 -2.4 5,700 - EX28DRAFT_4441 0.55 -0.4 5,730 - EX28DRAFT_4441 0.67 -1.4 5,736 + EX28DRAFT_4441 0.69 -0.3 5,757 - EX28DRAFT_4441 0.77 -2.2 5,857 + -1.0 5,858 - -3.2 5,929 + EX28DRAFT_4442 0.10 -0.2 5,929 + EX28DRAFT_4442 0.10 +0.4 5,930 - EX28DRAFT_4442 0.10 -2.0 5,935 - EX28DRAFT_4442 0.11 -3.9 5,942 + EX28DRAFT_4442 0.11 +1.0 5,943 - EX28DRAFT_4442 0.12 -1.7 5,943 - EX28DRAFT_4442 0.12 -2.4 5,962 + EX28DRAFT_4442 0.14 -0.8 5,963 - EX28DRAFT_4442 0.14 -5.1 6,182 - EX28DRAFT_4442 0.37 -2.9 6,184 - EX28DRAFT_4442 0.37 -4.3 6,329 - EX28DRAFT_4442 0.53 -3.2 6,407 - EX28DRAFT_4442 0.61 -4.4 6,407 - EX28DRAFT_4442 0.61 -5.7 6,407 - EX28DRAFT_4442 0.61 -5.0 6,453 + EX28DRAFT_4442 0.66 -1.6 6,546 - EX28DRAFT_4442 0.76 -2.6 6,546 - EX28DRAFT_4442 0.76 -4.2
Or see this region's nucleotide sequence