Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4420 and EX28DRAFT_4421 are separated by 9 nucleotides EX28DRAFT_4421 and EX28DRAFT_4422 are separated by 74 nucleotides EX28DRAFT_4422 and EX28DRAFT_4423 are separated by 129 nucleotides EX28DRAFT_4423 and EX28DRAFT_4424 are separated by 96 nucleotides
EX28DRAFT_4420: EX28DRAFT_4420 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 87,709 to 88,713
_4420
EX28DRAFT_4421: EX28DRAFT_4421 - N-acetylglutamate kinase (EC 2.7.2.8), at 88,723 to 89,496
_4421
EX28DRAFT_4422: EX28DRAFT_4422 - argininosuccinate lyase, at 89,571 to 90,944
_4422
EX28DRAFT_4423: EX28DRAFT_4423 - RNA, at 91,074 to 91,184
_4423
EX28DRAFT_4424: EX28DRAFT_4424 - Transcriptional regulator, at 91,281 to 92,198
_4424
Position (kb) 89
90
91 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 88.635 kb on - strand at 88.674 kb on + strand at 88.675 kb on - strand at 88.707 kb on + strand at 88.737 kb on + strand at 88.754 kb on + strand at 89.048 kb on + strand, within EX28DRAFT_4421 at 89.484 kb on + strand at 89.484 kb on + strand at 89.490 kb on - strand at 89.515 kb on + strand at 89.549 kb on + strand at 89.670 kb on - strand at 89.738 kb on - strand, within EX28DRAFT_4422 at 89.738 kb on - strand, within EX28DRAFT_4422 at 89.933 kb on + strand, within EX28DRAFT_4422 at 90.184 kb on - strand, within EX28DRAFT_4422 at 90.521 kb on + strand, within EX28DRAFT_4422 at 90.521 kb on + strand, within EX28DRAFT_4422 at 90.522 kb on - strand, within EX28DRAFT_4422 at 90.706 kb on + strand, within EX28DRAFT_4422 at 90.728 kb on + strand, within EX28DRAFT_4422 at 90.729 kb on - strand, within EX28DRAFT_4422 at 91.184 kb on + strand at 91.193 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 88,635 - -3.6 88,674 + -4.1 88,675 - -3.5 88,707 + -1.3 88,737 + -3.0 88,754 + -1.6 89,048 + EX28DRAFT_4421 0.42 -4.6 89,484 + -1.7 89,484 + -2.4 89,490 - -2.5 89,515 + +0.6 89,549 + +0.3 89,670 - -5.0 89,738 - EX28DRAFT_4422 0.12 -5.2 89,738 - EX28DRAFT_4422 0.12 -5.6 89,933 + EX28DRAFT_4422 0.26 -3.7 90,184 - EX28DRAFT_4422 0.45 -3.1 90,521 + EX28DRAFT_4422 0.69 -4.7 90,521 + EX28DRAFT_4422 0.69 -6.0 90,522 - EX28DRAFT_4422 0.69 -3.0 90,706 + EX28DRAFT_4422 0.83 -5.6 90,728 + EX28DRAFT_4422 0.84 -2.7 90,729 - EX28DRAFT_4422 0.84 -2.9 91,184 + -0.3 91,193 + +1.3
Or see this region's nucleotide sequence