Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4418 and EX28DRAFT_4419 are separated by 271 nucleotides EX28DRAFT_4419 and EX28DRAFT_4420 are separated by 88 nucleotides
EX28DRAFT_4418: EX28DRAFT_4418 - Phosphoenolpyruvate carboxylase, type 1 (EC 4.1.1.31), at 83,546 to 86,197
_4418
EX28DRAFT_4419: EX28DRAFT_4419 - acetylornithine deacetylase (ArgE), at 86,469 to 87,620
_4419
EX28DRAFT_4420: EX28DRAFT_4420 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 87,709 to 88,713
_4420
Position (kb)
86
87
88 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 85.540 kb on - strand, within EX28DRAFT_4418 at 85.543 kb on + strand, within EX28DRAFT_4418 at 85.544 kb on - strand, within EX28DRAFT_4418 at 85.544 kb on - strand, within EX28DRAFT_4418 at 86.167 kb on + strand at 86.170 kb on + strand at 86.198 kb on - strand at 86.276 kb on - strand at 86.285 kb on + strand at 86.332 kb on - strand at 86.332 kb on - strand at 86.374 kb on + strand at 86.471 kb on - strand at 86.632 kb on + strand, within EX28DRAFT_4419 at 86.633 kb on - strand, within EX28DRAFT_4419 at 87.163 kb on + strand, within EX28DRAFT_4419 at 87.211 kb on + strand, within EX28DRAFT_4419 at 87.634 kb on + strand at 87.634 kb on + strand at 87.634 kb on + strand at 87.634 kb on + strand at 87.634 kb on + strand at 87.635 kb on - strand at 87.635 kb on - strand at 87.635 kb on - strand at 87.635 kb on - strand at 87.645 kb on - strand at 87.647 kb on + strand at 87.654 kb on - strand at 87.697 kb on + strand at 87.697 kb on + strand at 87.697 kb on + strand at 87.698 kb on - strand at 87.698 kb on - strand at 87.718 kb on - strand at 87.761 kb on + strand at 87.762 kb on - strand at 87.766 kb on + strand at 87.786 kb on + strand at 87.787 kb on - strand at 87.787 kb on - strand at 88.132 kb on - strand, within EX28DRAFT_4420 at 88.327 kb on + strand, within EX28DRAFT_4420
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 85,540 - EX28DRAFT_4418 0.75 -2.6 85,543 + EX28DRAFT_4418 0.75 -2.1 85,544 - EX28DRAFT_4418 0.75 -4.2 85,544 - EX28DRAFT_4418 0.75 -3.4 86,167 + -1.8 86,170 + -3.4 86,198 - -4.5 86,276 - -1.5 86,285 + -2.4 86,332 - -0.1 86,332 - -0.7 86,374 + -3.6 86,471 - +0.6 86,632 + EX28DRAFT_4419 0.14 -0.8 86,633 - EX28DRAFT_4419 0.14 -3.6 87,163 + EX28DRAFT_4419 0.60 -2.9 87,211 + EX28DRAFT_4419 0.64 -1.2 87,634 + +0.1 87,634 + +0.1 87,634 + +0.2 87,634 + -0.5 87,634 + +0.2 87,635 - -1.1 87,635 - -3.8 87,635 - -2.6 87,635 - -1.3 87,645 - -2.1 87,647 + -0.2 87,654 - -3.1 87,697 + -0.1 87,697 + +0.4 87,697 + -3.6 87,698 - -3.7 87,698 - -4.9 87,718 - -4.2 87,761 + -3.7 87,762 - -5.6 87,766 + -4.0 87,786 + -3.6 87,787 - -2.6 87,787 - -1.6 88,132 - EX28DRAFT_4420 0.42 -3.5 88,327 + EX28DRAFT_4420 0.61 -4.5
Or see this region's nucleotide sequence