Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4416 and EX28DRAFT_4417 are separated by 128 nucleotides EX28DRAFT_4417 and EX28DRAFT_4418 are separated by 93 nucleotides EX28DRAFT_4418 and EX28DRAFT_4419 are separated by 271 nucleotides
EX28DRAFT_4416: EX28DRAFT_4416 - fructose-6-phosphate aldolase, TalC/MipB family, at 81,897 to 82,559
_4416
EX28DRAFT_4417: EX28DRAFT_4417 - Helix-turn-helix domain, at 82,688 to 83,452
_4417
EX28DRAFT_4418: EX28DRAFT_4418 - Phosphoenolpyruvate carboxylase, type 1 (EC 4.1.1.31), at 83,546 to 86,197
_4418
EX28DRAFT_4419: EX28DRAFT_4419 - acetylornithine deacetylase (ArgE), at 86,469 to 87,620
_4419
Position (kb)
83
84
85
86
87 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 82.571 kb on - strand at 82.589 kb on - strand at 82.595 kb on + strand at 82.596 kb on - strand at 82.728 kb on + strand at 82.776 kb on + strand, within EX28DRAFT_4417 at 82.776 kb on + strand, within EX28DRAFT_4417 at 82.777 kb on - strand, within EX28DRAFT_4417 at 82.777 kb on - strand, within EX28DRAFT_4417 at 82.789 kb on + strand, within EX28DRAFT_4417 at 82.823 kb on - strand, within EX28DRAFT_4417 at 82.823 kb on - strand, within EX28DRAFT_4417 at 82.831 kb on - strand, within EX28DRAFT_4417 at 82.878 kb on + strand, within EX28DRAFT_4417 at 82.927 kb on - strand, within EX28DRAFT_4417 at 82.941 kb on + strand, within EX28DRAFT_4417 at 83.091 kb on - strand, within EX28DRAFT_4417 at 83.132 kb on + strand, within EX28DRAFT_4417 at 83.132 kb on + strand, within EX28DRAFT_4417 at 83.133 kb on - strand, within EX28DRAFT_4417 at 83.133 kb on - strand, within EX28DRAFT_4417 at 83.209 kb on + strand, within EX28DRAFT_4417 at 83.210 kb on - strand, within EX28DRAFT_4417 at 83.210 kb on - strand, within EX28DRAFT_4417 at 83.222 kb on + strand, within EX28DRAFT_4417 at 83.255 kb on + strand, within EX28DRAFT_4417 at 83.255 kb on + strand, within EX28DRAFT_4417 at 83.255 kb on + strand, within EX28DRAFT_4417 at 83.255 kb on + strand, within EX28DRAFT_4417 at 83.256 kb on - strand, within EX28DRAFT_4417 at 83.256 kb on - strand, within EX28DRAFT_4417 at 83.256 kb on - strand, within EX28DRAFT_4417 at 83.256 kb on - strand, within EX28DRAFT_4417 at 83.307 kb on - strand, within EX28DRAFT_4417 at 83.361 kb on + strand, within EX28DRAFT_4417 at 83.362 kb on - strand, within EX28DRAFT_4417 at 83.441 kb on - strand at 83.508 kb on + strand at 83.509 kb on - strand at 83.509 kb on - strand at 83.544 kb on + strand at 83.544 kb on + strand at 83.545 kb on - strand at 83.558 kb on - strand at 83.626 kb on + strand at 83.626 kb on + strand at 83.695 kb on - strand at 83.853 kb on + strand, within EX28DRAFT_4418 at 84.072 kb on - strand, within EX28DRAFT_4418 at 84.211 kb on + strand, within EX28DRAFT_4418 at 84.336 kb on + strand, within EX28DRAFT_4418 at 84.607 kb on + strand, within EX28DRAFT_4418 at 84.784 kb on - strand, within EX28DRAFT_4418 at 85.014 kb on - strand, within EX28DRAFT_4418 at 85.014 kb on - strand, within EX28DRAFT_4418 at 85.118 kb on - strand, within EX28DRAFT_4418 at 85.118 kb on - strand, within EX28DRAFT_4418 at 85.540 kb on - strand, within EX28DRAFT_4418 at 85.543 kb on + strand, within EX28DRAFT_4418 at 85.544 kb on - strand, within EX28DRAFT_4418 at 85.544 kb on - strand, within EX28DRAFT_4418 at 86.167 kb on + strand at 86.170 kb on + strand at 86.198 kb on - strand at 86.276 kb on - strand at 86.285 kb on + strand at 86.332 kb on - strand at 86.332 kb on - strand at 86.374 kb on + strand at 86.471 kb on - strand at 86.632 kb on + strand, within EX28DRAFT_4419 at 86.633 kb on - strand, within EX28DRAFT_4419 at 87.163 kb on + strand, within EX28DRAFT_4419
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 82,571 - +0.3 82,589 - -2.0 82,595 + +0.2 82,596 - +0.2 82,728 + +0.1 82,776 + EX28DRAFT_4417 0.12 -0.6 82,776 + EX28DRAFT_4417 0.12 +0.7 82,777 - EX28DRAFT_4417 0.12 +0.6 82,777 - EX28DRAFT_4417 0.12 -0.7 82,789 + EX28DRAFT_4417 0.13 -2.9 82,823 - EX28DRAFT_4417 0.18 +0.5 82,823 - EX28DRAFT_4417 0.18 -0.1 82,831 - EX28DRAFT_4417 0.19 +0.8 82,878 + EX28DRAFT_4417 0.25 +0.2 82,927 - EX28DRAFT_4417 0.31 +0.9 82,941 + EX28DRAFT_4417 0.33 -0.4 83,091 - EX28DRAFT_4417 0.53 -0.1 83,132 + EX28DRAFT_4417 0.58 -0.8 83,132 + EX28DRAFT_4417 0.58 -0.3 83,133 - EX28DRAFT_4417 0.58 +0.2 83,133 - EX28DRAFT_4417 0.58 +0.8 83,209 + EX28DRAFT_4417 0.68 +1.2 83,210 - EX28DRAFT_4417 0.68 -0.3 83,210 - EX28DRAFT_4417 0.68 +0.4 83,222 + EX28DRAFT_4417 0.70 -0.2 83,255 + EX28DRAFT_4417 0.74 -2.1 83,255 + EX28DRAFT_4417 0.74 +0.2 83,255 + EX28DRAFT_4417 0.74 -0.1 83,255 + EX28DRAFT_4417 0.74 -0.1 83,256 - EX28DRAFT_4417 0.74 -0.6 83,256 - EX28DRAFT_4417 0.74 -0.4 83,256 - EX28DRAFT_4417 0.74 +0.9 83,256 - EX28DRAFT_4417 0.74 -0.0 83,307 - EX28DRAFT_4417 0.81 -0.5 83,361 + EX28DRAFT_4417 0.88 +0.5 83,362 - EX28DRAFT_4417 0.88 +0.5 83,441 - -0.3 83,508 + +0.7 83,509 - +1.0 83,509 - +0.2 83,544 + -0.2 83,544 + -1.0 83,545 - -1.0 83,558 - -3.5 83,626 + -3.5 83,626 + -2.7 83,695 - -4.1 83,853 + EX28DRAFT_4418 0.12 -2.4 84,072 - EX28DRAFT_4418 0.20 -2.6 84,211 + EX28DRAFT_4418 0.25 -3.1 84,336 + EX28DRAFT_4418 0.30 +0.4 84,607 + EX28DRAFT_4418 0.40 -4.3 84,784 - EX28DRAFT_4418 0.47 -2.6 85,014 - EX28DRAFT_4418 0.55 -4.5 85,014 - EX28DRAFT_4418 0.55 -3.5 85,118 - EX28DRAFT_4418 0.59 -2.6 85,118 - EX28DRAFT_4418 0.59 -4.1 85,540 - EX28DRAFT_4418 0.75 -2.6 85,543 + EX28DRAFT_4418 0.75 -2.1 85,544 - EX28DRAFT_4418 0.75 -4.2 85,544 - EX28DRAFT_4418 0.75 -3.4 86,167 + -1.8 86,170 + -3.4 86,198 - -4.5 86,276 - -1.5 86,285 + -2.4 86,332 - -0.1 86,332 - -0.7 86,374 + -3.6 86,471 - +0.6 86,632 + EX28DRAFT_4419 0.14 -0.8 86,633 - EX28DRAFT_4419 0.14 -3.6 87,163 + EX28DRAFT_4419 0.60 -2.9
Or see this region's nucleotide sequence