Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4381 and EX28DRAFT_4382 overlap by 1 nucleotides EX28DRAFT_4382 and EX28DRAFT_4383 are separated by 103 nucleotides EX28DRAFT_4383 and EX28DRAFT_4384 are separated by 203 nucleotides EX28DRAFT_4384 and EX28DRAFT_4385 are separated by 71 nucleotides
EX28DRAFT_4381: EX28DRAFT_4381 - Branched-chain amino acid transport protein (AzlD), at 45,788 to 46,111
_4381
EX28DRAFT_4382: EX28DRAFT_4382 - Predicted branched-chain amino acid permease (azaleucine resistance), at 46,111 to 46,770
_4382
EX28DRAFT_4383: EX28DRAFT_4383 - transcriptional regulator, XRE family with cupin sensor, at 46,874 to 47,422
_4383
EX28DRAFT_4384: EX28DRAFT_4384 - Superoxide dismutase, at 47,626 to 48,246
_4384
EX28DRAFT_4385: EX28DRAFT_4385 - Uncharacterized protein conserved in bacteria, at 48,318 to 48,992
_4385
Position (kb)
46
47
48 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 46.056 kb on + strand, within EX28DRAFT_4381 at 46.056 kb on + strand, within EX28DRAFT_4381 at 46.056 kb on + strand, within EX28DRAFT_4381 at 46.056 kb on + strand, within EX28DRAFT_4381 at 46.057 kb on - strand, within EX28DRAFT_4381 at 46.276 kb on + strand, within EX28DRAFT_4382 at 46.378 kb on + strand, within EX28DRAFT_4382 at 46.379 kb on - strand, within EX28DRAFT_4382 at 46.379 kb on - strand, within EX28DRAFT_4382 at 46.590 kb on + strand, within EX28DRAFT_4382 at 46.596 kb on + strand, within EX28DRAFT_4382 at 46.633 kb on + strand, within EX28DRAFT_4382 at 46.633 kb on + strand, within EX28DRAFT_4382 at 46.661 kb on + strand, within EX28DRAFT_4382 at 46.683 kb on - strand, within EX28DRAFT_4382 at 46.733 kb on - strand at 46.770 kb on + strand at 46.871 kb on + strand at 46.871 kb on + strand at 46.871 kb on + strand at 46.871 kb on + strand at 46.972 kb on + strand, within EX28DRAFT_4383 at 47.056 kb on - strand, within EX28DRAFT_4383 at 47.119 kb on + strand, within EX28DRAFT_4383 at 47.119 kb on + strand, within EX28DRAFT_4383 at 47.196 kb on + strand, within EX28DRAFT_4383 at 47.197 kb on - strand, within EX28DRAFT_4383 at 47.197 kb on - strand, within EX28DRAFT_4383 at 47.197 kb on - strand, within EX28DRAFT_4383 at 47.197 kb on - strand, within EX28DRAFT_4383 at 47.199 kb on - strand, within EX28DRAFT_4383 at 47.199 kb on - strand, within EX28DRAFT_4383 at 47.209 kb on + strand, within EX28DRAFT_4383 at 47.267 kb on - strand, within EX28DRAFT_4383 at 47.267 kb on - strand, within EX28DRAFT_4383 at 47.267 kb on - strand, within EX28DRAFT_4383 at 47.269 kb on + strand, within EX28DRAFT_4383 at 47.269 kb on + strand, within EX28DRAFT_4383 at 47.270 kb on - strand, within EX28DRAFT_4383 at 47.270 kb on - strand, within EX28DRAFT_4383 at 47.270 kb on - strand, within EX28DRAFT_4383 at 47.336 kb on + strand, within EX28DRAFT_4383 at 47.337 kb on - strand, within EX28DRAFT_4383 at 47.337 kb on - strand, within EX28DRAFT_4383 at 47.337 kb on - strand, within EX28DRAFT_4383 at 47.555 kb on - strand at 47.558 kb on + strand at 47.562 kb on + strand at 47.563 kb on - strand at 47.563 kb on - strand at 47.563 kb on - strand at 47.564 kb on + strand at 47.626 kb on - strand at 47.632 kb on + strand at 47.634 kb on + strand at 47.635 kb on - strand at 47.728 kb on + strand, within EX28DRAFT_4384 at 47.844 kb on + strand, within EX28DRAFT_4384 at 48.145 kb on + strand, within EX28DRAFT_4384 at 48.148 kb on + strand, within EX28DRAFT_4384 at 48.178 kb on + strand, within EX28DRAFT_4384 at 48.179 kb on - strand, within EX28DRAFT_4384 at 48.245 kb on - strand at 48.278 kb on + strand at 48.296 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 46,056 + EX28DRAFT_4381 0.83 -0.0 46,056 + EX28DRAFT_4381 0.83 -0.2 46,056 + EX28DRAFT_4381 0.83 -0.3 46,056 + EX28DRAFT_4381 0.83 +0.2 46,057 - EX28DRAFT_4381 0.83 +0.5 46,276 + EX28DRAFT_4382 0.25 +0.1 46,378 + EX28DRAFT_4382 0.40 +0.6 46,379 - EX28DRAFT_4382 0.41 -0.0 46,379 - EX28DRAFT_4382 0.41 +0.6 46,590 + EX28DRAFT_4382 0.73 -0.2 46,596 + EX28DRAFT_4382 0.73 +0.2 46,633 + EX28DRAFT_4382 0.79 +0.5 46,633 + EX28DRAFT_4382 0.79 +1.0 46,661 + EX28DRAFT_4382 0.83 -0.0 46,683 - EX28DRAFT_4382 0.87 +0.5 46,733 - +0.1 46,770 + +0.2 46,871 + +0.5 46,871 + -1.2 46,871 + +0.1 46,871 + +0.1 46,972 + EX28DRAFT_4383 0.18 +0.7 47,056 - EX28DRAFT_4383 0.33 -1.7 47,119 + EX28DRAFT_4383 0.45 +0.2 47,119 + EX28DRAFT_4383 0.45 +0.3 47,196 + EX28DRAFT_4383 0.59 -0.9 47,197 - EX28DRAFT_4383 0.59 -1.2 47,197 - EX28DRAFT_4383 0.59 -3.3 47,197 - EX28DRAFT_4383 0.59 -0.6 47,197 - EX28DRAFT_4383 0.59 -0.3 47,199 - EX28DRAFT_4383 0.59 +0.2 47,199 - EX28DRAFT_4383 0.59 -0.4 47,209 + EX28DRAFT_4383 0.61 -0.5 47,267 - EX28DRAFT_4383 0.72 -0.9 47,267 - EX28DRAFT_4383 0.72 -0.7 47,267 - EX28DRAFT_4383 0.72 -0.6 47,269 + EX28DRAFT_4383 0.72 +0.3 47,269 + EX28DRAFT_4383 0.72 -0.8 47,270 - EX28DRAFT_4383 0.72 -0.6 47,270 - EX28DRAFT_4383 0.72 -0.5 47,270 - EX28DRAFT_4383 0.72 -2.7 47,336 + EX28DRAFT_4383 0.84 +0.1 47,337 - EX28DRAFT_4383 0.84 -0.5 47,337 - EX28DRAFT_4383 0.84 -0.6 47,337 - EX28DRAFT_4383 0.84 -0.4 47,555 - -0.1 47,558 + -0.6 47,562 + -2.6 47,563 - +0.0 47,563 - -0.5 47,563 - +0.5 47,564 + +0.2 47,626 - +0.8 47,632 + +0.3 47,634 + +0.0 47,635 - +0.4 47,728 + EX28DRAFT_4384 0.16 +0.9 47,844 + EX28DRAFT_4384 0.35 -0.8 48,145 + EX28DRAFT_4384 0.84 -0.6 48,148 + EX28DRAFT_4384 0.84 -0.1 48,178 + EX28DRAFT_4384 0.89 +0.6 48,179 - EX28DRAFT_4384 0.89 +0.4 48,245 - +0.4 48,278 + +0.3 48,296 + -1.2
Or see this region's nucleotide sequence