Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4091 and EX28DRAFT_4092 overlap by 4 nucleotides EX28DRAFT_4092 and EX28DRAFT_4093 overlap by 4 nucleotides EX28DRAFT_4093 and EX28DRAFT_4094 are separated by 1 nucleotides EX28DRAFT_4094 and EX28DRAFT_4095 overlap by 4 nucleotides
EX28DRAFT_4091: EX28DRAFT_4091 - 3-oxoacyl-(acyl-carrier-protein) reductase, putative, at 65,601 to 66,332
_4091
EX28DRAFT_4092: EX28DRAFT_4092 - Predicted 3-hydroxylacyl-(acyl carrier protein) dehydratase, at 66,329 to 66,802
_4092
EX28DRAFT_4093: EX28DRAFT_4093 - 3-oxoacyl-(acyl-carrier-protein) synthase, at 66,799 to 67,968
_4093
EX28DRAFT_4094: EX28DRAFT_4094 - Protein of unknown function (DUF3261), at 67,970 to 68,548
_4094
EX28DRAFT_4095: EX28DRAFT_4095 - Predicted exporter, at 68,545 to 70,869
_4095
Position (kb) 66
67
68 Strain fitness (log2 ratio) -2
-1
0
1 at 65.943 kb on - strand, within EX28DRAFT_4091 at 65.963 kb on + strand, within EX28DRAFT_4091 at 65.964 kb on - strand, within EX28DRAFT_4091 at 65.964 kb on - strand, within EX28DRAFT_4091 at 65.984 kb on + strand, within EX28DRAFT_4091 at 65.986 kb on + strand, within EX28DRAFT_4091 at 66.081 kb on + strand, within EX28DRAFT_4091 at 66.089 kb on - strand, within EX28DRAFT_4091 at 66.389 kb on - strand, within EX28DRAFT_4092 at 66.562 kb on + strand, within EX28DRAFT_4092 at 66.778 kb on - strand at 66.778 kb on - strand at 66.791 kb on + strand at 66.791 kb on + strand at 66.791 kb on + strand at 66.792 kb on - strand at 66.792 kb on - strand at 66.795 kb on + strand at 66.795 kb on + strand at 66.795 kb on + strand at 66.796 kb on - strand at 66.796 kb on - strand at 66.796 kb on - strand at 66.796 kb on - strand at 66.856 kb on - strand at 67.006 kb on - strand, within EX28DRAFT_4093 at 67.085 kb on - strand, within EX28DRAFT_4093 at 67.139 kb on + strand, within EX28DRAFT_4093 at 67.231 kb on - strand, within EX28DRAFT_4093 at 67.235 kb on + strand, within EX28DRAFT_4093 at 67.236 kb on - strand, within EX28DRAFT_4093 at 67.270 kb on + strand, within EX28DRAFT_4093 at 67.271 kb on - strand, within EX28DRAFT_4093 at 67.271 kb on - strand, within EX28DRAFT_4093 at 67.292 kb on - strand, within EX28DRAFT_4093 at 67.292 kb on - strand, within EX28DRAFT_4093 at 67.871 kb on + strand at 67.871 kb on + strand at 67.872 kb on - strand at 67.872 kb on - strand at 67.872 kb on - strand at 67.959 kb on + strand at 67.960 kb on - strand at 67.960 kb on - strand at 67.961 kb on + strand at 67.961 kb on + strand at 67.961 kb on + strand at 67.962 kb on - strand at 67.962 kb on - strand at 67.983 kb on + strand at 67.984 kb on - strand at 67.984 kb on - strand at 68.001 kb on + strand at 68.001 kb on + strand at 68.002 kb on - strand at 68.243 kb on - strand, within EX28DRAFT_4094 at 68.262 kb on + strand, within EX28DRAFT_4094 at 68.550 kb on + strand at 68.762 kb on + strand at 68.763 kb on - strand at 68.766 kb on + strand at 68.954 kb on - strand, within EX28DRAFT_4095
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 65,943 - EX28DRAFT_4091 0.47 +0.1 65,963 + EX28DRAFT_4091 0.49 -0.2 65,964 - EX28DRAFT_4091 0.50 +0.5 65,964 - EX28DRAFT_4091 0.50 +0.1 65,984 + EX28DRAFT_4091 0.52 +0.2 65,986 + EX28DRAFT_4091 0.53 +0.4 66,081 + EX28DRAFT_4091 0.66 -0.1 66,089 - EX28DRAFT_4091 0.67 +0.2 66,389 - EX28DRAFT_4092 0.13 -1.7 66,562 + EX28DRAFT_4092 0.49 -0.3 66,778 - -2.0 66,778 - +1.1 66,791 + +0.2 66,791 + +0.3 66,791 + -0.7 66,792 - -1.0 66,792 - -0.1 66,795 + -0.0 66,795 + -0.5 66,795 + -0.6 66,796 - -0.6 66,796 - -0.0 66,796 - +1.0 66,796 - +0.4 66,856 - -0.2 67,006 - EX28DRAFT_4093 0.18 -0.4 67,085 - EX28DRAFT_4093 0.24 -0.7 67,139 + EX28DRAFT_4093 0.29 +0.3 67,231 - EX28DRAFT_4093 0.37 +0.9 67,235 + EX28DRAFT_4093 0.37 +0.0 67,236 - EX28DRAFT_4093 0.37 -0.3 67,270 + EX28DRAFT_4093 0.40 -0.1 67,271 - EX28DRAFT_4093 0.40 -0.6 67,271 - EX28DRAFT_4093 0.40 -0.1 67,292 - EX28DRAFT_4093 0.42 -1.0 67,292 - EX28DRAFT_4093 0.42 -0.3 67,871 + -0.6 67,871 + +1.4 67,872 - +0.8 67,872 - +0.1 67,872 - +0.1 67,959 + +0.8 67,960 - +0.1 67,960 - +0.2 67,961 + -0.4 67,961 + +1.4 67,961 + +0.7 67,962 - -0.7 67,962 - +0.3 67,983 + -1.0 67,984 - +0.2 67,984 - +0.3 68,001 + -0.1 68,001 + +1.0 68,002 - +1.1 68,243 - EX28DRAFT_4094 0.47 -0.8 68,262 + EX28DRAFT_4094 0.50 +0.5 68,550 + -0.3 68,762 + +0.4 68,763 - -0.4 68,766 + -1.0 68,954 - EX28DRAFT_4095 0.18 +0.2
Or see this region's nucleotide sequence