Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4070 and EX28DRAFT_4071 are separated by 32 nucleotides EX28DRAFT_4071 and EX28DRAFT_4072 overlap by 1 nucleotides EX28DRAFT_4072 and EX28DRAFT_4073 are separated by 169 nucleotides EX28DRAFT_4073 and EX28DRAFT_4074 are separated by 37 nucleotides
EX28DRAFT_4070: EX28DRAFT_4070 - flavoprotein, HI0933 family, at 45,122 to 46,318
_4070
EX28DRAFT_4071: EX28DRAFT_4071 - Phosphoglycerate dehydrogenase and related dehydrogenases, at 46,351 to 47,289
_4071
EX28DRAFT_4072: EX28DRAFT_4072 - Demethylmenaquinone methyltransferase, at 47,289 to 47,981
_4072
EX28DRAFT_4073: EX28DRAFT_4073 - transcriptional regulator, IclR family, at 48,151 to 48,888
_4073
EX28DRAFT_4074: EX28DRAFT_4074 - Sugar phosphate permease, at 48,926 to 50,260
_4074
Position (kb)
47
48 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 46.321 kb on + strand at 46.322 kb on - strand at 46.322 kb on - strand at 46.322 kb on - strand at 46.389 kb on + strand at 46.389 kb on + strand at 46.390 kb on - strand at 46.390 kb on - strand at 46.419 kb on + strand at 46.419 kb on + strand at 46.419 kb on + strand at 46.421 kb on + strand at 46.421 kb on + strand at 46.422 kb on - strand at 46.422 kb on - strand at 46.447 kb on - strand, within EX28DRAFT_4071 at 46.600 kb on - strand, within EX28DRAFT_4071 at 46.645 kb on - strand, within EX28DRAFT_4071 at 46.758 kb on + strand, within EX28DRAFT_4071 at 46.758 kb on + strand, within EX28DRAFT_4071 at 46.759 kb on - strand, within EX28DRAFT_4071 at 46.772 kb on + strand, within EX28DRAFT_4071 at 46.965 kb on + strand, within EX28DRAFT_4071 at 46.965 kb on + strand, within EX28DRAFT_4071 at 46.966 kb on - strand, within EX28DRAFT_4071 at 46.966 kb on - strand, within EX28DRAFT_4071 at 47.131 kb on + strand, within EX28DRAFT_4071 at 47.131 kb on + strand, within EX28DRAFT_4071 at 47.161 kb on - strand, within EX28DRAFT_4071 at 47.209 kb on + strand at 47.224 kb on + strand at 47.225 kb on - strand at 47.273 kb on - strand at 47.315 kb on + strand at 47.315 kb on + strand at 47.316 kb on - strand at 47.316 kb on - strand at 47.316 kb on - strand at 47.316 kb on - strand at 47.316 kb on - strand at 47.544 kb on + strand, within EX28DRAFT_4072 at 47.590 kb on + strand, within EX28DRAFT_4072 at 47.623 kb on - strand, within EX28DRAFT_4072 at 47.636 kb on + strand, within EX28DRAFT_4072 at 47.636 kb on + strand, within EX28DRAFT_4072 at 47.637 kb on - strand, within EX28DRAFT_4072 at 47.639 kb on + strand, within EX28DRAFT_4072 at 47.639 kb on + strand, within EX28DRAFT_4072 at 47.639 kb on + strand, within EX28DRAFT_4072 at 47.903 kb on - strand, within EX28DRAFT_4072 at 47.981 kb on + strand at 47.981 kb on + strand at 47.982 kb on - strand at 47.982 kb on - strand at 48.059 kb on + strand at 48.088 kb on + strand at 48.088 kb on + strand at 48.089 kb on - strand at 48.118 kb on + strand at 48.147 kb on - strand at 48.170 kb on + strand at 48.310 kb on + strand, within EX28DRAFT_4073 at 48.362 kb on - strand, within EX28DRAFT_4073 at 48.376 kb on + strand, within EX28DRAFT_4073 at 48.376 kb on + strand, within EX28DRAFT_4073 at 48.460 kb on + strand, within EX28DRAFT_4073 at 48.460 kb on + strand, within EX28DRAFT_4073 at 48.461 kb on - strand, within EX28DRAFT_4073 at 48.461 kb on - strand, within EX28DRAFT_4073 at 48.578 kb on + strand, within EX28DRAFT_4073 at 48.579 kb on - strand, within EX28DRAFT_4073 at 48.579 kb on - strand, within EX28DRAFT_4073 at 48.638 kb on - strand, within EX28DRAFT_4073 at 48.773 kb on + strand, within EX28DRAFT_4073 at 48.774 kb on - strand, within EX28DRAFT_4073 at 48.829 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 46,321 + +2.4 46,322 - -0.4 46,322 - -2.6 46,322 - +0.0 46,389 + +0.8 46,389 + +0.2 46,390 - +0.4 46,390 - +0.4 46,419 + -0.1 46,419 + +0.3 46,419 + -0.1 46,421 + +1.6 46,421 + -0.1 46,422 - -0.4 46,422 - +0.2 46,447 - EX28DRAFT_4071 0.10 +0.2 46,600 - EX28DRAFT_4071 0.27 -0.1 46,645 - EX28DRAFT_4071 0.31 -0.5 46,758 + EX28DRAFT_4071 0.43 +0.1 46,758 + EX28DRAFT_4071 0.43 -0.4 46,759 - EX28DRAFT_4071 0.43 -0.6 46,772 + EX28DRAFT_4071 0.45 +1.7 46,965 + EX28DRAFT_4071 0.65 -0.0 46,965 + EX28DRAFT_4071 0.65 -0.5 46,966 - EX28DRAFT_4071 0.65 -0.3 46,966 - EX28DRAFT_4071 0.65 +0.4 47,131 + EX28DRAFT_4071 0.83 -0.5 47,131 + EX28DRAFT_4071 0.83 -0.1 47,161 - EX28DRAFT_4071 0.86 -0.4 47,209 + -0.1 47,224 + -0.7 47,225 - -0.1 47,273 - +0.2 47,315 + +0.6 47,315 + -1.2 47,316 - +0.5 47,316 - +0.5 47,316 - -0.2 47,316 - -1.8 47,316 - +0.4 47,544 + EX28DRAFT_4072 0.37 -1.5 47,590 + EX28DRAFT_4072 0.43 +0.5 47,623 - EX28DRAFT_4072 0.48 +0.4 47,636 + EX28DRAFT_4072 0.50 +0.2 47,636 + EX28DRAFT_4072 0.50 +0.0 47,637 - EX28DRAFT_4072 0.50 +0.1 47,639 + EX28DRAFT_4072 0.51 +0.0 47,639 + EX28DRAFT_4072 0.51 -0.3 47,639 + EX28DRAFT_4072 0.51 -0.3 47,903 - EX28DRAFT_4072 0.89 -0.1 47,981 + -0.7 47,981 + +0.2 47,982 - -1.0 47,982 - +0.8 48,059 + +1.0 48,088 + +0.7 48,088 + +1.0 48,089 - +1.1 48,118 + -1.2 48,147 - +1.2 48,170 + +1.1 48,310 + EX28DRAFT_4073 0.22 +0.3 48,362 - EX28DRAFT_4073 0.29 +0.6 48,376 + EX28DRAFT_4073 0.30 +1.3 48,376 + EX28DRAFT_4073 0.30 -0.2 48,460 + EX28DRAFT_4073 0.42 +0.6 48,460 + EX28DRAFT_4073 0.42 +0.1 48,461 - EX28DRAFT_4073 0.42 -0.0 48,461 - EX28DRAFT_4073 0.42 +0.3 48,578 + EX28DRAFT_4073 0.58 -1.6 48,579 - EX28DRAFT_4073 0.58 -0.7 48,579 - EX28DRAFT_4073 0.58 +0.2 48,638 - EX28DRAFT_4073 0.66 +0.7 48,773 + EX28DRAFT_4073 0.84 -0.0 48,774 - EX28DRAFT_4073 0.84 +0.2 48,829 - -0.6
Or see this region's nucleotide sequence