Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3973 and EX28DRAFT_3974 are separated by 136 nucleotides EX28DRAFT_3974 and EX28DRAFT_3975 overlap by 7 nucleotides EX28DRAFT_3975 and EX28DRAFT_3976 are separated by 251 nucleotides EX28DRAFT_3976 and EX28DRAFT_3977 overlap by 4 nucleotides
EX28DRAFT_3973: EX28DRAFT_3973 - Molecular chaperone (small heat shock protein), at 182,403 to 182,831
_3973
EX28DRAFT_3974: EX28DRAFT_3974 - Molecular chaperone (small heat shock protein), at 182,968 to 183,378
_3974
EX28DRAFT_3975: EX28DRAFT_3975 - RNA, at 183,372 to 183,443
_3975
EX28DRAFT_3976: EX28DRAFT_3976 - Predicted periplasmic lipoprotein, at 183,695 to 184,024
_3976
EX28DRAFT_3977: EX28DRAFT_3977 - Protein of unknown function (DUF3748), at 184,021 to 185,292
_3977
Position (kb) 183
184
185 Strain fitness (log2 ratio) -2
-1
0
1 at 182.728 kb on + strand, within EX28DRAFT_3973 at 182.815 kb on - strand at 182.815 kb on - strand at 182.822 kb on - strand at 182.825 kb on + strand at 182.826 kb on - strand at 182.835 kb on - strand at 182.835 kb on - strand at 182.869 kb on + strand at 182.870 kb on - strand at 182.914 kb on - strand at 182.965 kb on + strand at 182.966 kb on - strand at 182.969 kb on + strand at 182.970 kb on - strand at 183.128 kb on + strand, within EX28DRAFT_3974 at 183.128 kb on + strand, within EX28DRAFT_3974 at 183.128 kb on + strand, within EX28DRAFT_3974 at 183.129 kb on - strand, within EX28DRAFT_3974 at 183.129 kb on - strand, within EX28DRAFT_3974 at 183.132 kb on + strand, within EX28DRAFT_3974 at 183.133 kb on - strand, within EX28DRAFT_3974 at 183.133 kb on - strand, within EX28DRAFT_3974 at 183.133 kb on - strand, within EX28DRAFT_3974 at 183.272 kb on + strand, within EX28DRAFT_3974 at 183.273 kb on - strand, within EX28DRAFT_3974 at 183.351 kb on - strand at 183.353 kb on - strand at 183.378 kb on + strand at 183.582 kb on - strand at 183.613 kb on + strand at 183.613 kb on + strand at 183.614 kb on - strand at 183.614 kb on - strand at 183.632 kb on + strand at 183.663 kb on + strand at 183.664 kb on - strand at 183.664 kb on - strand at 183.677 kb on - strand at 183.741 kb on + strand, within EX28DRAFT_3976 at 183.741 kb on - strand, within EX28DRAFT_3976 at 183.795 kb on - strand, within EX28DRAFT_3976 at 183.839 kb on - strand, within EX28DRAFT_3976 at 184.025 kb on + strand at 184.254 kb on + strand, within EX28DRAFT_3977 at 184.329 kb on + strand, within EX28DRAFT_3977 at 184.330 kb on - strand, within EX28DRAFT_3977 at 184.429 kb on - strand, within EX28DRAFT_3977 at 184.543 kb on + strand, within EX28DRAFT_3977 at 184.544 kb on - strand, within EX28DRAFT_3977 at 184.634 kb on + strand, within EX28DRAFT_3977 at 184.823 kb on + strand, within EX28DRAFT_3977 at 184.824 kb on - strand, within EX28DRAFT_3977 at 184.824 kb on - strand, within EX28DRAFT_3977 at 184.828 kb on - strand, within EX28DRAFT_3977 at 185.024 kb on + strand, within EX28DRAFT_3977 at 185.024 kb on + strand, within EX28DRAFT_3977 at 185.024 kb on + strand, within EX28DRAFT_3977
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 182,728 + EX28DRAFT_3973 0.76 +0.3 182,815 - +0.4 182,815 - +0.2 182,822 - -0.4 182,825 + -0.7 182,826 - -0.0 182,835 - +0.1 182,835 - +0.2 182,869 + -0.2 182,870 - -0.1 182,914 - +0.0 182,965 + -0.2 182,966 - +0.1 182,969 + -0.6 182,970 - -0.7 183,128 + EX28DRAFT_3974 0.39 -2.4 183,128 + EX28DRAFT_3974 0.39 +0.6 183,128 + EX28DRAFT_3974 0.39 -0.0 183,129 - EX28DRAFT_3974 0.39 +0.2 183,129 - EX28DRAFT_3974 0.39 +0.0 183,132 + EX28DRAFT_3974 0.40 -0.3 183,133 - EX28DRAFT_3974 0.40 -0.0 183,133 - EX28DRAFT_3974 0.40 +0.4 183,133 - EX28DRAFT_3974 0.40 -0.9 183,272 + EX28DRAFT_3974 0.74 -0.1 183,273 - EX28DRAFT_3974 0.74 -0.5 183,351 - +0.4 183,353 - -0.3 183,378 + +0.7 183,582 - -0.1 183,613 + +0.8 183,613 + -0.5 183,614 - -1.0 183,614 - +0.1 183,632 + -0.7 183,663 + -0.0 183,664 - +0.1 183,664 - +0.1 183,677 - +0.2 183,741 + EX28DRAFT_3976 0.14 -1.6 183,741 - EX28DRAFT_3976 0.14 -0.7 183,795 - EX28DRAFT_3976 0.30 +0.9 183,839 - EX28DRAFT_3976 0.44 -0.5 184,025 + -1.0 184,254 + EX28DRAFT_3977 0.18 +0.4 184,329 + EX28DRAFT_3977 0.24 +0.9 184,330 - EX28DRAFT_3977 0.24 +0.3 184,429 - EX28DRAFT_3977 0.32 +0.5 184,543 + EX28DRAFT_3977 0.41 -0.4 184,544 - EX28DRAFT_3977 0.41 -0.1 184,634 + EX28DRAFT_3977 0.48 +0.2 184,823 + EX28DRAFT_3977 0.63 +0.1 184,824 - EX28DRAFT_3977 0.63 +0.9 184,824 - EX28DRAFT_3977 0.63 -1.2 184,828 - EX28DRAFT_3977 0.63 -0.1 185,024 + EX28DRAFT_3977 0.79 -1.4 185,024 + EX28DRAFT_3977 0.79 +0.2 185,024 + EX28DRAFT_3977 0.79 -0.0
Or see this region's nucleotide sequence