Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3820 and EX28DRAFT_3821 are separated by 331 nucleotides EX28DRAFT_3821 and EX28DRAFT_3822 are separated by 39 nucleotides EX28DRAFT_3822 and EX28DRAFT_3823 are separated by 255 nucleotides
EX28DRAFT_3820: EX28DRAFT_3820 - Protein of unknown function (DUF3053), at 23,387 to 24,097
_3820
EX28DRAFT_3821: EX28DRAFT_3821 - Predicted transcriptional regulator, at 24,429 to 24,716
_3821
EX28DRAFT_3822: EX28DRAFT_3822 - ATPase components of ABC transporters with duplicated ATPase domains, at 24,756 to 26,375
_3822
EX28DRAFT_3823: EX28DRAFT_3823 - glycyl-tRNA synthetase beta chain (EC 6.1.1.14), at 26,631 to 28,700
_3823
Position (kb) 24
25
26
27 Strain fitness (log2 ratio) -2
-1
0
1
2 at 23.785 kb on + strand, within EX28DRAFT_3820 at 23.785 kb on + strand, within EX28DRAFT_3820 at 23.785 kb on + strand, within EX28DRAFT_3820 at 23.785 kb on + strand, within EX28DRAFT_3820 at 23.786 kb on - strand, within EX28DRAFT_3820 at 23.786 kb on - strand, within EX28DRAFT_3820 at 23.787 kb on + strand, within EX28DRAFT_3820 at 23.787 kb on + strand, within EX28DRAFT_3820 at 23.787 kb on + strand, within EX28DRAFT_3820 at 23.787 kb on + strand, within EX28DRAFT_3820 at 23.788 kb on - strand, within EX28DRAFT_3820 at 23.825 kb on + strand, within EX28DRAFT_3820 at 23.969 kb on - strand, within EX28DRAFT_3820 at 24.100 kb on + strand at 24.101 kb on - strand at 24.185 kb on - strand at 24.185 kb on - strand at 24.259 kb on + strand at 24.259 kb on + strand at 24.260 kb on - strand at 24.260 kb on - strand at 24.266 kb on - strand at 24.278 kb on - strand at 24.282 kb on + strand at 24.283 kb on - strand at 24.286 kb on + strand at 24.286 kb on + strand at 24.286 kb on + strand at 24.287 kb on - strand at 24.307 kb on + strand at 24.359 kb on + strand at 24.360 kb on - strand at 24.369 kb on + strand at 24.369 kb on + strand at 24.414 kb on + strand at 24.435 kb on + strand at 24.435 kb on + strand at 24.438 kb on - strand at 24.438 kb on - strand at 24.494 kb on - strand, within EX28DRAFT_3821 at 24.540 kb on + strand, within EX28DRAFT_3821 at 24.541 kb on - strand, within EX28DRAFT_3821 at 24.541 kb on - strand, within EX28DRAFT_3821 at 24.541 kb on - strand, within EX28DRAFT_3821 at 24.662 kb on - strand, within EX28DRAFT_3821 at 24.662 kb on - strand, within EX28DRAFT_3821 at 24.709 kb on + strand at 24.710 kb on - strand at 24.814 kb on - strand at 24.870 kb on + strand at 24.870 kb on + strand at 24.870 kb on + strand at 24.871 kb on - strand at 24.937 kb on - strand, within EX28DRAFT_3822 at 24.987 kb on - strand, within EX28DRAFT_3822 at 25.091 kb on + strand, within EX28DRAFT_3822 at 25.092 kb on - strand, within EX28DRAFT_3822 at 25.092 kb on - strand, within EX28DRAFT_3822 at 25.092 kb on - strand, within EX28DRAFT_3822 at 25.225 kb on - strand, within EX28DRAFT_3822 at 25.397 kb on + strand, within EX28DRAFT_3822 at 25.425 kb on - strand, within EX28DRAFT_3822 at 25.505 kb on + strand, within EX28DRAFT_3822 at 25.505 kb on + strand, within EX28DRAFT_3822 at 25.506 kb on - strand, within EX28DRAFT_3822 at 25.507 kb on + strand, within EX28DRAFT_3822 at 25.508 kb on - strand, within EX28DRAFT_3822 at 25.532 kb on + strand, within EX28DRAFT_3822 at 25.687 kb on + strand, within EX28DRAFT_3822 at 25.820 kb on - strand, within EX28DRAFT_3822 at 25.820 kb on - strand, within EX28DRAFT_3822 at 26.054 kb on - strand, within EX28DRAFT_3822 at 26.180 kb on + strand, within EX28DRAFT_3822 at 26.375 kb on + strand at 26.601 kb on + strand at 26.601 kb on + strand at 26.602 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 23,785 + EX28DRAFT_3820 0.56 +0.2 23,785 + EX28DRAFT_3820 0.56 +0.4 23,785 + EX28DRAFT_3820 0.56 -0.3 23,785 + EX28DRAFT_3820 0.56 +0.8 23,786 - EX28DRAFT_3820 0.56 -0.0 23,786 - EX28DRAFT_3820 0.56 +0.6 23,787 + EX28DRAFT_3820 0.56 -0.8 23,787 + EX28DRAFT_3820 0.56 -0.0 23,787 + EX28DRAFT_3820 0.56 -2.6 23,787 + EX28DRAFT_3820 0.56 -0.3 23,788 - EX28DRAFT_3820 0.56 +0.3 23,825 + EX28DRAFT_3820 0.62 +0.1 23,969 - EX28DRAFT_3820 0.82 +0.1 24,100 + -0.6 24,101 - +0.5 24,185 - +0.8 24,185 - +0.1 24,259 + +0.4 24,259 + +0.4 24,260 - +0.8 24,260 - +0.4 24,266 - +0.4 24,278 - +0.7 24,282 + +0.4 24,283 - -1.2 24,286 + +0.4 24,286 + +0.2 24,286 + +1.8 24,287 - +0.2 24,307 + -0.2 24,359 + +0.2 24,360 - +1.6 24,369 + +0.4 24,369 + +0.1 24,414 + -1.0 24,435 + -0.9 24,435 + +0.2 24,438 - +0.1 24,438 - +0.4 24,494 - EX28DRAFT_3821 0.23 +0.1 24,540 + EX28DRAFT_3821 0.39 +1.9 24,541 - EX28DRAFT_3821 0.39 +0.3 24,541 - EX28DRAFT_3821 0.39 -0.3 24,541 - EX28DRAFT_3821 0.39 -1.3 24,662 - EX28DRAFT_3821 0.81 -0.3 24,662 - EX28DRAFT_3821 0.81 -0.1 24,709 + +0.1 24,710 - +0.7 24,814 - +0.7 24,870 + +0.4 24,870 + +1.4 24,870 + -0.2 24,871 - -0.3 24,937 - EX28DRAFT_3822 0.11 +0.5 24,987 - EX28DRAFT_3822 0.14 +0.2 25,091 + EX28DRAFT_3822 0.21 +0.4 25,092 - EX28DRAFT_3822 0.21 -1.1 25,092 - EX28DRAFT_3822 0.21 -0.6 25,092 - EX28DRAFT_3822 0.21 +0.6 25,225 - EX28DRAFT_3822 0.29 -0.2 25,397 + EX28DRAFT_3822 0.40 -2.0 25,425 - EX28DRAFT_3822 0.41 -0.5 25,505 + EX28DRAFT_3822 0.46 +0.4 25,505 + EX28DRAFT_3822 0.46 +1.2 25,506 - EX28DRAFT_3822 0.46 +0.5 25,507 + EX28DRAFT_3822 0.46 +0.2 25,508 - EX28DRAFT_3822 0.46 -0.0 25,532 + EX28DRAFT_3822 0.48 +0.1 25,687 + EX28DRAFT_3822 0.57 +0.2 25,820 - EX28DRAFT_3822 0.66 +0.1 25,820 - EX28DRAFT_3822 0.66 -1.0 26,054 - EX28DRAFT_3822 0.80 +0.1 26,180 + EX28DRAFT_3822 0.88 +1.0 26,375 + -0.5 26,601 + -0.3 26,601 + -2.1 26,602 - +0.0
Or see this region's nucleotide sequence