Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3724 and EX28DRAFT_3725 overlap by 4 nucleotides EX28DRAFT_3725 and EX28DRAFT_3726 are separated by 104 nucleotides EX28DRAFT_3726 and EX28DRAFT_3727 are separated by 62 nucleotides
EX28DRAFT_3724: EX28DRAFT_3724 - ABC-type multidrug transport system, ATPase component, at 400,361 to 401,287
_3724
EX28DRAFT_3725: EX28DRAFT_3725 - ABC-type polysaccharide/polyol phosphate export systems, permease component, at 401,284 to 402,054
_3725
EX28DRAFT_3726: EX28DRAFT_3726 - Phosphotransferase system, mannose/fructose-specific component IIA, at 402,159 to 402,599
_3726
EX28DRAFT_3727: EX28DRAFT_3727 - Predicted xylanase/chitin deacetylase, at 402,662 to 403,909
_3727
Position (kb) 401
402
403 Strain fitness (log2 ratio) -2
-1
0
1
2 at 400.293 kb on - strand at 400.295 kb on + strand at 400.296 kb on - strand at 400.297 kb on + strand at 400.297 kb on + strand at 400.350 kb on - strand at 400.354 kb on + strand at 400.354 kb on + strand at 400.390 kb on + strand at 400.431 kb on + strand at 400.432 kb on - strand at 400.460 kb on + strand, within EX28DRAFT_3724 at 400.690 kb on + strand, within EX28DRAFT_3724 at 400.723 kb on + strand, within EX28DRAFT_3724 at 401.103 kb on - strand, within EX28DRAFT_3724 at 401.108 kb on + strand, within EX28DRAFT_3724 at 401.164 kb on + strand, within EX28DRAFT_3724 at 401.208 kb on + strand at 401.208 kb on + strand at 401.212 kb on + strand at 401.219 kb on - strand at 401.259 kb on + strand at 401.260 kb on - strand at 401.344 kb on - strand at 401.349 kb on + strand at 401.350 kb on - strand at 401.395 kb on + strand, within EX28DRAFT_3725 at 401.396 kb on - strand, within EX28DRAFT_3725 at 401.451 kb on + strand, within EX28DRAFT_3725 at 401.451 kb on + strand, within EX28DRAFT_3725 at 401.452 kb on - strand, within EX28DRAFT_3725 at 401.452 kb on - strand, within EX28DRAFT_3725 at 401.452 kb on - strand, within EX28DRAFT_3725 at 401.455 kb on + strand, within EX28DRAFT_3725 at 401.455 kb on + strand, within EX28DRAFT_3725 at 401.455 kb on + strand, within EX28DRAFT_3725 at 401.455 kb on + strand, within EX28DRAFT_3725 at 401.456 kb on - strand, within EX28DRAFT_3725 at 401.456 kb on - strand, within EX28DRAFT_3725 at 401.456 kb on - strand, within EX28DRAFT_3725 at 401.457 kb on + strand, within EX28DRAFT_3725 at 401.458 kb on - strand, within EX28DRAFT_3725 at 401.519 kb on + strand, within EX28DRAFT_3725 at 401.519 kb on + strand, within EX28DRAFT_3725 at 401.520 kb on - strand, within EX28DRAFT_3725 at 401.849 kb on - strand, within EX28DRAFT_3725 at 401.908 kb on + strand, within EX28DRAFT_3725 at 401.908 kb on + strand, within EX28DRAFT_3725 at 401.909 kb on - strand, within EX28DRAFT_3725 at 401.992 kb on - strand at 402.005 kb on - strand at 402.102 kb on + strand at 402.147 kb on - strand at 402.158 kb on + strand at 402.158 kb on + strand at 402.159 kb on - strand at 402.159 kb on - strand at 402.159 kb on - strand at 402.159 kb on - strand at 402.212 kb on + strand, within EX28DRAFT_3726 at 402.402 kb on - strand, within EX28DRAFT_3726 at 402.402 kb on - strand, within EX28DRAFT_3726 at 402.435 kb on - strand, within EX28DRAFT_3726 at 402.435 kb on - strand, within EX28DRAFT_3726 at 402.435 kb on - strand, within EX28DRAFT_3726 at 402.460 kb on - strand, within EX28DRAFT_3726 at 402.589 kb on - strand at 402.597 kb on + strand at 402.626 kb on - strand at 402.764 kb on - strand at 402.866 kb on + strand, within EX28DRAFT_3727 at 402.866 kb on + strand, within EX28DRAFT_3727 at 402.997 kb on + strand, within EX28DRAFT_3727 at 402.998 kb on - strand, within EX28DRAFT_3727 at 403.020 kb on - strand, within EX28DRAFT_3727
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 400,293 - -0.2 400,295 + +0.4 400,296 - +0.7 400,297 + +0.1 400,297 + -0.1 400,350 - +1.0 400,354 + -1.4 400,354 + -1.4 400,390 + -0.1 400,431 + -2.0 400,432 - +0.3 400,460 + EX28DRAFT_3724 0.11 +1.2 400,690 + EX28DRAFT_3724 0.35 +0.4 400,723 + EX28DRAFT_3724 0.39 +1.0 401,103 - EX28DRAFT_3724 0.80 +0.3 401,108 + EX28DRAFT_3724 0.81 +0.5 401,164 + EX28DRAFT_3724 0.87 +0.2 401,208 + +0.4 401,208 + +0.4 401,212 + +0.2 401,219 - -1.8 401,259 + +0.8 401,260 - -1.1 401,344 - +0.2 401,349 + +2.2 401,350 - -1.2 401,395 + EX28DRAFT_3725 0.14 +0.7 401,396 - EX28DRAFT_3725 0.15 -0.3 401,451 + EX28DRAFT_3725 0.22 +0.2 401,451 + EX28DRAFT_3725 0.22 +0.2 401,452 - EX28DRAFT_3725 0.22 +0.2 401,452 - EX28DRAFT_3725 0.22 +0.4 401,452 - EX28DRAFT_3725 0.22 +0.4 401,455 + EX28DRAFT_3725 0.22 +0.1 401,455 + EX28DRAFT_3725 0.22 +0.3 401,455 + EX28DRAFT_3725 0.22 +0.6 401,455 + EX28DRAFT_3725 0.22 +0.7 401,456 - EX28DRAFT_3725 0.22 +0.0 401,456 - EX28DRAFT_3725 0.22 +0.5 401,456 - EX28DRAFT_3725 0.22 -0.2 401,457 + EX28DRAFT_3725 0.22 +0.2 401,458 - EX28DRAFT_3725 0.23 +0.5 401,519 + EX28DRAFT_3725 0.30 +0.1 401,519 + EX28DRAFT_3725 0.30 -0.1 401,520 - EX28DRAFT_3725 0.31 +1.4 401,849 - EX28DRAFT_3725 0.73 +0.4 401,908 + EX28DRAFT_3725 0.81 -0.4 401,908 + EX28DRAFT_3725 0.81 +0.8 401,909 - EX28DRAFT_3725 0.81 -0.2 401,992 - +1.2 402,005 - -0.1 402,102 + -0.4 402,147 - +0.3 402,158 + +0.2 402,158 + +0.2 402,159 - -0.0 402,159 - +1.4 402,159 - +0.2 402,159 - +0.2 402,212 + EX28DRAFT_3726 0.12 -0.3 402,402 - EX28DRAFT_3726 0.55 +0.6 402,402 - EX28DRAFT_3726 0.55 +0.2 402,435 - EX28DRAFT_3726 0.63 -0.4 402,435 - EX28DRAFT_3726 0.63 -0.5 402,435 - EX28DRAFT_3726 0.63 +0.0 402,460 - EX28DRAFT_3726 0.68 +0.2 402,589 - +0.3 402,597 + +0.6 402,626 - +0.1 402,764 - -0.6 402,866 + EX28DRAFT_3727 0.16 -0.2 402,866 + EX28DRAFT_3727 0.16 -0.2 402,997 + EX28DRAFT_3727 0.27 +0.2 402,998 - EX28DRAFT_3727 0.27 -0.4 403,020 - EX28DRAFT_3727 0.29 -0.1
Or see this region's nucleotide sequence