Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3700 and EX28DRAFT_3701 are separated by 10 nucleotides EX28DRAFT_3701 and EX28DRAFT_3702 are separated by 174 nucleotides EX28DRAFT_3702 and EX28DRAFT_3703 are separated by 87 nucleotides EX28DRAFT_3703 and EX28DRAFT_3704 are separated by 9 nucleotides
EX28DRAFT_3700: EX28DRAFT_3700 - Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB, at 370,509 to 371,891
_3700
EX28DRAFT_3701: EX28DRAFT_3701 - prepilin-type N-terminal cleavage/methylation domain, at 371,902 to 372,339
_3701
EX28DRAFT_3702: EX28DRAFT_3702 - nicotinate-nucleotide pyrophosphorylase [carboxylating] (EC 2.4.2.19), at 372,514 to 373,407
_3702
EX28DRAFT_3703: EX28DRAFT_3703 - N-acetylmuramoyl-L-alanine amidase, at 373,495 to 374,046
_3703
EX28DRAFT_3704: EX28DRAFT_3704 - Membrane protein required for beta-lactamase induction, at 374,056 to 374,910
_3704
Position (kb) 372
373
374 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 371.635 kb on + strand, within EX28DRAFT_3700 at 371.645 kb on + strand, within EX28DRAFT_3700 at 371.698 kb on - strand, within EX28DRAFT_3700 at 371.965 kb on - strand, within EX28DRAFT_3701 at 372.124 kb on - strand, within EX28DRAFT_3701 at 372.217 kb on - strand, within EX28DRAFT_3701 at 372.339 kb on + strand at 372.340 kb on - strand at 372.340 kb on - strand at 372.350 kb on + strand at 372.578 kb on + strand at 372.578 kb on + strand at 372.578 kb on + strand at 372.578 kb on + strand at 372.579 kb on - strand at 372.579 kb on - strand at 372.579 kb on - strand at 372.766 kb on + strand, within EX28DRAFT_3702 at 372.838 kb on - strand, within EX28DRAFT_3702 at 372.838 kb on - strand, within EX28DRAFT_3702 at 372.838 kb on - strand, within EX28DRAFT_3702 at 372.915 kb on - strand, within EX28DRAFT_3702 at 372.915 kb on - strand, within EX28DRAFT_3702 at 372.915 kb on - strand, within EX28DRAFT_3702 at 373.347 kb on + strand at 373.350 kb on + strand at 373.350 kb on + strand at 373.350 kb on + strand at 373.350 kb on + strand at 373.350 kb on + strand at 373.351 kb on - strand at 373.351 kb on - strand at 373.351 kb on - strand at 373.351 kb on - strand at 373.351 kb on - strand at 373.351 kb on - strand at 373.354 kb on - strand at 373.391 kb on + strand at 373.410 kb on + strand at 373.411 kb on - strand at 373.411 kb on - strand at 373.437 kb on - strand at 373.452 kb on + strand at 374.045 kb on - strand at 374.052 kb on + strand at 374.052 kb on + strand at 374.052 kb on + strand at 374.068 kb on - strand at 374.214 kb on + strand, within EX28DRAFT_3704 at 374.214 kb on + strand, within EX28DRAFT_3704 at 374.214 kb on + strand, within EX28DRAFT_3704 at 374.215 kb on - strand, within EX28DRAFT_3704 at 374.239 kb on + strand, within EX28DRAFT_3704 at 374.335 kb on + strand, within EX28DRAFT_3704 at 374.336 kb on - strand, within EX28DRAFT_3704
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 371,635 + EX28DRAFT_3700 0.81 -1.2 371,645 + EX28DRAFT_3700 0.82 +0.7 371,698 - EX28DRAFT_3700 0.86 +0.3 371,965 - EX28DRAFT_3701 0.14 -1.5 372,124 - EX28DRAFT_3701 0.51 +1.4 372,217 - EX28DRAFT_3701 0.72 -0.0 372,339 + +0.1 372,340 - -0.2 372,340 - +0.5 372,350 + +0.1 372,578 + -0.9 372,578 + -1.7 372,578 + -1.4 372,578 + -1.4 372,579 - -1.4 372,579 - -1.8 372,579 - -2.8 372,766 + EX28DRAFT_3702 0.28 +1.2 372,838 - EX28DRAFT_3702 0.36 -2.6 372,838 - EX28DRAFT_3702 0.36 -1.6 372,838 - EX28DRAFT_3702 0.36 -0.0 372,915 - EX28DRAFT_3702 0.45 -1.3 372,915 - EX28DRAFT_3702 0.45 -1.8 372,915 - EX28DRAFT_3702 0.45 +0.6 373,347 + -1.4 373,350 + -0.4 373,350 + -3.3 373,350 + -1.4 373,350 + -0.8 373,350 + -1.8 373,351 - +1.2 373,351 - -1.2 373,351 - -0.4 373,351 - -1.9 373,351 - +0.4 373,351 - -0.8 373,354 - -1.8 373,391 + -2.1 373,410 + -1.2 373,411 - -0.1 373,411 - -2.1 373,437 - -0.6 373,452 + -0.6 374,045 - +0.1 374,052 + +0.0 374,052 + -0.1 374,052 + -0.1 374,068 - +0.1 374,214 + EX28DRAFT_3704 0.18 +0.6 374,214 + EX28DRAFT_3704 0.18 +0.4 374,214 + EX28DRAFT_3704 0.18 +0.0 374,215 - EX28DRAFT_3704 0.19 +0.2 374,239 + EX28DRAFT_3704 0.21 +0.5 374,335 + EX28DRAFT_3704 0.33 -0.1 374,336 - EX28DRAFT_3704 0.33 +0.8
Or see this region's nucleotide sequence