Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3673 and EX28DRAFT_3674 are separated by 1 nucleotides EX28DRAFT_3674 and EX28DRAFT_3675 are separated by 179 nucleotides EX28DRAFT_3675 and EX28DRAFT_3676 are separated by 563 nucleotides
EX28DRAFT_3673: EX28DRAFT_3673 - acetolactate synthase, large subunit (EC 2.2.1.6), at 340,567 to 342,291
_3673
EX28DRAFT_3674: EX28DRAFT_3674 - acetolactate synthase, small subunit (EC 2.2.1.6), at 342,293 to 342,784
_3674
EX28DRAFT_3675: EX28DRAFT_3675 - transcriptional regulator, LacI family, at 342,964 to 343,971
_3675
EX28DRAFT_3676: EX28DRAFT_3676 - mraZ protein, at 344,535 to 345,029
_3676
Position (kb) 342
343
344 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 342.025 kb on - strand, within EX28DRAFT_3673 at 342.031 kb on + strand, within EX28DRAFT_3673 at 342.032 kb on - strand, within EX28DRAFT_3673 at 342.055 kb on + strand, within EX28DRAFT_3673 at 342.055 kb on + strand, within EX28DRAFT_3673 at 342.056 kb on - strand, within EX28DRAFT_3673 at 342.056 kb on - strand, within EX28DRAFT_3673 at 342.098 kb on - strand, within EX28DRAFT_3673 at 342.107 kb on + strand, within EX28DRAFT_3673 at 342.108 kb on - strand, within EX28DRAFT_3673 at 342.108 kb on - strand, within EX28DRAFT_3673 at 342.192 kb on + strand at 342.237 kb on + strand at 342.237 kb on + strand at 342.292 kb on + strand at 342.374 kb on + strand, within EX28DRAFT_3674 at 342.487 kb on + strand, within EX28DRAFT_3674 at 342.487 kb on + strand, within EX28DRAFT_3674 at 342.534 kb on - strand, within EX28DRAFT_3674 at 342.650 kb on + strand, within EX28DRAFT_3674 at 342.651 kb on - strand, within EX28DRAFT_3674 at 342.651 kb on - strand, within EX28DRAFT_3674 at 342.783 kb on - strand at 342.863 kb on - strand at 342.895 kb on + strand at 343.018 kb on + strand at 343.019 kb on - strand at 343.098 kb on + strand, within EX28DRAFT_3675 at 343.098 kb on + strand, within EX28DRAFT_3675 at 343.099 kb on - strand, within EX28DRAFT_3675 at 343.099 kb on - strand, within EX28DRAFT_3675 at 343.099 kb on - strand, within EX28DRAFT_3675 at 343.102 kb on + strand, within EX28DRAFT_3675 at 343.102 kb on + strand, within EX28DRAFT_3675 at 343.152 kb on + strand, within EX28DRAFT_3675 at 343.190 kb on - strand, within EX28DRAFT_3675 at 343.604 kb on - strand, within EX28DRAFT_3675 at 343.604 kb on - strand, within EX28DRAFT_3675 at 343.609 kb on + strand, within EX28DRAFT_3675 at 343.609 kb on + strand, within EX28DRAFT_3675 at 343.609 kb on + strand, within EX28DRAFT_3675 at 343.617 kb on + strand, within EX28DRAFT_3675 at 343.618 kb on - strand, within EX28DRAFT_3675 at 343.622 kb on - strand, within EX28DRAFT_3675 at 343.622 kb on - strand, within EX28DRAFT_3675 at 343.749 kb on + strand, within EX28DRAFT_3675 at 344.025 kb on - strand at 344.042 kb on + strand at 344.043 kb on - strand at 344.064 kb on + strand at 344.065 kb on - strand at 344.065 kb on - strand at 344.183 kb on - strand at 344.318 kb on - strand at 344.319 kb on + strand at 344.320 kb on - strand at 344.324 kb on - strand at 344.386 kb on + strand at 344.386 kb on + strand at 344.386 kb on + strand at 344.442 kb on - strand at 344.442 kb on - strand at 344.450 kb on - strand at 344.474 kb on + strand at 344.690 kb on + strand, within EX28DRAFT_3676
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 342,025 - EX28DRAFT_3673 0.85 -0.3 342,031 + EX28DRAFT_3673 0.85 +0.5 342,032 - EX28DRAFT_3673 0.85 -0.5 342,055 + EX28DRAFT_3673 0.86 +1.3 342,055 + EX28DRAFT_3673 0.86 +1.0 342,056 - EX28DRAFT_3673 0.86 +1.7 342,056 - EX28DRAFT_3673 0.86 +0.5 342,098 - EX28DRAFT_3673 0.89 -1.9 342,107 + EX28DRAFT_3673 0.89 -0.2 342,108 - EX28DRAFT_3673 0.89 -0.8 342,108 - EX28DRAFT_3673 0.89 +1.0 342,192 + -0.1 342,237 + -0.3 342,237 + -0.2 342,292 + -0.9 342,374 + EX28DRAFT_3674 0.16 +0.5 342,487 + EX28DRAFT_3674 0.39 +0.1 342,487 + EX28DRAFT_3674 0.39 +2.8 342,534 - EX28DRAFT_3674 0.49 -2.8 342,650 + EX28DRAFT_3674 0.73 +0.2 342,651 - EX28DRAFT_3674 0.73 +0.0 342,651 - EX28DRAFT_3674 0.73 +0.8 342,783 - -0.1 342,863 - +0.5 342,895 + -0.3 343,018 + -1.5 343,019 - -1.0 343,098 + EX28DRAFT_3675 0.13 -1.9 343,098 + EX28DRAFT_3675 0.13 -1.4 343,099 - EX28DRAFT_3675 0.13 -2.2 343,099 - EX28DRAFT_3675 0.13 -1.1 343,099 - EX28DRAFT_3675 0.13 -1.0 343,102 + EX28DRAFT_3675 0.14 -0.5 343,102 + EX28DRAFT_3675 0.14 -1.4 343,152 + EX28DRAFT_3675 0.19 -1.8 343,190 - EX28DRAFT_3675 0.22 +0.2 343,604 - EX28DRAFT_3675 0.63 -1.8 343,604 - EX28DRAFT_3675 0.63 -0.7 343,609 + EX28DRAFT_3675 0.64 -1.9 343,609 + EX28DRAFT_3675 0.64 -1.1 343,609 + EX28DRAFT_3675 0.64 -0.4 343,617 + EX28DRAFT_3675 0.65 -2.1 343,618 - EX28DRAFT_3675 0.65 -0.2 343,622 - EX28DRAFT_3675 0.65 -2.8 343,622 - EX28DRAFT_3675 0.65 -1.7 343,749 + EX28DRAFT_3675 0.78 -1.4 344,025 - +0.4 344,042 + +0.8 344,043 - -0.1 344,064 + +0.3 344,065 - -0.0 344,065 - -2.1 344,183 - +0.4 344,318 - +0.6 344,319 + -1.7 344,320 - -0.7 344,324 - -1.9 344,386 + -0.0 344,386 + +0.2 344,386 + -0.7 344,442 - +0.3 344,442 - +0.9 344,450 - -1.2 344,474 + -0.2 344,690 + EX28DRAFT_3676 0.31 -0.1
Or see this region's nucleotide sequence