Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3666 and EX28DRAFT_3667 are separated by 162 nucleotides EX28DRAFT_3667 and EX28DRAFT_3668 are separated by 10 nucleotides EX28DRAFT_3668 and EX28DRAFT_3669 are separated by 2 nucleotides
EX28DRAFT_3666: EX28DRAFT_3666 - cation diffusion facilitator family transporter, at 332,723 to 333,613
_3666
EX28DRAFT_3667: EX28DRAFT_3667 - 3-isopropylmalate dehydratase, small subunit, at 333,776 to 334,381
_3667
EX28DRAFT_3668: EX28DRAFT_3668 - 3-isopropylmalate dehydratase, large subunit, at 334,392 to 335,792
_3668
EX28DRAFT_3669: EX28DRAFT_3669 - 3-isopropylmalate dehydrogenase, at 335,795 to 336,886
_3669
Position (kb) 334
335
336 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 333.413 kb on - strand, within EX28DRAFT_3666 at 333.444 kb on - strand, within EX28DRAFT_3666 at 333.498 kb on - strand, within EX28DRAFT_3666 at 333.536 kb on + strand at 333.558 kb on + strand at 333.558 kb on + strand at 333.558 kb on + strand at 333.559 kb on - strand at 333.563 kb on - strand at 333.566 kb on + strand at 333.675 kb on + strand at 333.675 kb on + strand at 333.710 kb on + strand at 333.711 kb on - strand at 333.714 kb on + strand at 333.715 kb on - strand at 333.903 kb on + strand, within EX28DRAFT_3667 at 334.095 kb on + strand, within EX28DRAFT_3667 at 334.306 kb on + strand, within EX28DRAFT_3667 at 334.307 kb on - strand, within EX28DRAFT_3667 at 334.309 kb on + strand, within EX28DRAFT_3667 at 334.357 kb on + strand at 334.357 kb on + strand at 334.357 kb on + strand at 334.393 kb on + strand at 334.394 kb on - strand at 334.503 kb on - strand at 334.666 kb on - strand, within EX28DRAFT_3668 at 334.666 kb on - strand, within EX28DRAFT_3668 at 334.772 kb on + strand, within EX28DRAFT_3668 at 334.772 kb on + strand, within EX28DRAFT_3668 at 334.819 kb on + strand, within EX28DRAFT_3668 at 334.819 kb on + strand, within EX28DRAFT_3668 at 334.819 kb on + strand, within EX28DRAFT_3668 at 334.819 kb on + strand, within EX28DRAFT_3668 at 334.819 kb on + strand, within EX28DRAFT_3668 at 334.819 kb on + strand, within EX28DRAFT_3668 at 334.819 kb on + strand, within EX28DRAFT_3668 at 335.068 kb on + strand, within EX28DRAFT_3668 at 335.068 kb on + strand, within EX28DRAFT_3668 at 335.130 kb on - strand, within EX28DRAFT_3668 at 335.501 kb on - strand, within EX28DRAFT_3668 at 335.716 kb on + strand at 335.747 kb on - strand at 335.776 kb on + strand at 335.776 kb on + strand at 335.776 kb on + strand at 335.778 kb on + strand at 335.779 kb on - strand at 335.787 kb on - strand at 335.796 kb on + strand at 335.796 kb on + strand at 335.797 kb on - strand at 335.815 kb on - strand at 336.213 kb on + strand, within EX28DRAFT_3669 at 336.213 kb on + strand, within EX28DRAFT_3669 at 336.213 kb on + strand, within EX28DRAFT_3669 at 336.213 kb on + strand, within EX28DRAFT_3669 at 336.213 kb on + strand, within EX28DRAFT_3669 at 336.214 kb on - strand, within EX28DRAFT_3669 at 336.323 kb on - strand, within EX28DRAFT_3669 at 336.323 kb on - strand, within EX28DRAFT_3669 at 336.388 kb on - strand, within EX28DRAFT_3669 at 336.388 kb on - strand, within EX28DRAFT_3669 at 336.388 kb on - strand, within EX28DRAFT_3669 at 336.760 kb on - strand, within EX28DRAFT_3669
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 333,413 - EX28DRAFT_3666 0.77 +0.5 333,444 - EX28DRAFT_3666 0.81 +0.2 333,498 - EX28DRAFT_3666 0.87 +0.6 333,536 + +0.2 333,558 + -0.1 333,558 + +0.2 333,558 + -0.4 333,559 - +2.2 333,563 - -2.1 333,566 + +0.6 333,675 + +0.6 333,675 + +0.1 333,710 + -0.3 333,711 - +0.1 333,714 + +1.5 333,715 - -0.7 333,903 + EX28DRAFT_3667 0.21 -5.9 334,095 + EX28DRAFT_3667 0.53 -4.7 334,306 + EX28DRAFT_3667 0.87 -1.5 334,307 - EX28DRAFT_3667 0.88 -2.7 334,309 + EX28DRAFT_3667 0.88 -0.8 334,357 + -4.3 334,357 + -5.0 334,357 + -3.6 334,393 + -4.9 334,394 - +0.5 334,503 - -4.8 334,666 - EX28DRAFT_3668 0.20 -4.7 334,666 - EX28DRAFT_3668 0.20 -0.4 334,772 + EX28DRAFT_3668 0.27 -2.9 334,772 + EX28DRAFT_3668 0.27 -4.3 334,819 + EX28DRAFT_3668 0.30 -3.7 334,819 + EX28DRAFT_3668 0.30 -2.4 334,819 + EX28DRAFT_3668 0.30 -3.3 334,819 + EX28DRAFT_3668 0.30 -4.6 334,819 + EX28DRAFT_3668 0.30 -4.3 334,819 + EX28DRAFT_3668 0.30 -4.5 334,819 + EX28DRAFT_3668 0.30 -4.8 335,068 + EX28DRAFT_3668 0.48 -4.3 335,068 + EX28DRAFT_3668 0.48 -4.9 335,130 - EX28DRAFT_3668 0.53 -3.7 335,501 - EX28DRAFT_3668 0.79 -4.3 335,716 + -2.4 335,747 - -3.6 335,776 + -0.8 335,776 + -3.8 335,776 + -2.6 335,778 + -4.6 335,779 - +0.2 335,787 - -1.4 335,796 + -1.8 335,796 + -4.3 335,797 - -3.9 335,815 - -3.2 336,213 + EX28DRAFT_3669 0.38 -3.4 336,213 + EX28DRAFT_3669 0.38 -2.9 336,213 + EX28DRAFT_3669 0.38 -5.0 336,213 + EX28DRAFT_3669 0.38 -3.7 336,213 + EX28DRAFT_3669 0.38 -1.6 336,214 - EX28DRAFT_3669 0.38 -5.7 336,323 - EX28DRAFT_3669 0.48 -3.7 336,323 - EX28DRAFT_3669 0.48 -3.5 336,388 - EX28DRAFT_3669 0.54 -2.4 336,388 - EX28DRAFT_3669 0.54 -2.4 336,388 - EX28DRAFT_3669 0.54 -3.5 336,760 - EX28DRAFT_3669 0.88 -4.4
Or see this region's nucleotide sequence