Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3645 and EX28DRAFT_3646 overlap by 4 nucleotides EX28DRAFT_3646 and EX28DRAFT_3647 overlap by 1 nucleotides EX28DRAFT_3647 and EX28DRAFT_3648 are separated by 53 nucleotides
EX28DRAFT_3645: EX28DRAFT_3645 - ribosomal RNA small subunit methyltransferase A, at 306,560 to 307,381
_3645
EX28DRAFT_3646: EX28DRAFT_3646 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 307,378 to 308,364
_3646
EX28DRAFT_3647: EX28DRAFT_3647 - periplasmic chaperone for outer membrane proteins SurA, at 308,364 to 309,650
_3647
EX28DRAFT_3648: EX28DRAFT_3648 - Organic solvent tolerance protein OstA, at 309,704 to 312,055
_3648
Position (kb) 308
309
310 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 307.389 kb on + strand at 307.423 kb on - strand at 307.423 kb on - strand at 307.575 kb on - strand, within EX28DRAFT_3646 at 307.575 kb on - strand, within EX28DRAFT_3646 at 308.081 kb on + strand, within EX28DRAFT_3646 at 308.081 kb on + strand, within EX28DRAFT_3646 at 308.081 kb on + strand, within EX28DRAFT_3646 at 308.082 kb on - strand, within EX28DRAFT_3646 at 308.207 kb on - strand, within EX28DRAFT_3646 at 308.490 kb on - strand at 308.609 kb on + strand, within EX28DRAFT_3647 at 308.946 kb on - strand, within EX28DRAFT_3647 at 309.047 kb on + strand, within EX28DRAFT_3647 at 309.268 kb on + strand, within EX28DRAFT_3647 at 309.277 kb on + strand, within EX28DRAFT_3647 at 309.293 kb on - strand, within EX28DRAFT_3647 at 309.318 kb on + strand, within EX28DRAFT_3647 at 309.326 kb on + strand, within EX28DRAFT_3647 at 309.326 kb on + strand, within EX28DRAFT_3647 at 309.327 kb on - strand, within EX28DRAFT_3647 at 309.327 kb on - strand, within EX28DRAFT_3647 at 309.327 kb on - strand, within EX28DRAFT_3647 at 309.327 kb on - strand, within EX28DRAFT_3647 at 309.573 kb on - strand at 309.603 kb on - strand at 309.765 kb on + strand at 309.766 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 307,389 + +2.8 307,423 - -3.8 307,423 - -3.3 307,575 - EX28DRAFT_3646 0.20 -3.0 307,575 - EX28DRAFT_3646 0.20 -3.5 308,081 + EX28DRAFT_3646 0.71 -4.0 308,081 + EX28DRAFT_3646 0.71 -1.4 308,081 + EX28DRAFT_3646 0.71 -1.8 308,082 - EX28DRAFT_3646 0.71 -1.2 308,207 - EX28DRAFT_3646 0.84 -4.5 308,490 - -1.4 308,609 + EX28DRAFT_3647 0.19 -2.0 308,946 - EX28DRAFT_3647 0.45 -3.0 309,047 + EX28DRAFT_3647 0.53 -2.3 309,268 + EX28DRAFT_3647 0.70 -3.5 309,277 + EX28DRAFT_3647 0.71 -2.6 309,293 - EX28DRAFT_3647 0.72 -1.2 309,318 + EX28DRAFT_3647 0.74 -3.1 309,326 + EX28DRAFT_3647 0.75 -4.6 309,326 + EX28DRAFT_3647 0.75 -3.9 309,327 - EX28DRAFT_3647 0.75 -2.4 309,327 - EX28DRAFT_3647 0.75 -0.4 309,327 - EX28DRAFT_3647 0.75 -1.1 309,327 - EX28DRAFT_3647 0.75 +0.2 309,573 - -1.8 309,603 - -2.3 309,765 + -3.2 309,766 - -0.5
Or see this region's nucleotide sequence