Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3614 and EX28DRAFT_3615 are separated by 1 nucleotides EX28DRAFT_3615 and EX28DRAFT_3616 are separated by 3 nucleotides EX28DRAFT_3616 and EX28DRAFT_3617 are separated by 257 nucleotides EX28DRAFT_3617 and EX28DRAFT_3618 are separated by 32 nucleotides
EX28DRAFT_3614: EX28DRAFT_3614 - aspartate kinase (EC 2.7.2.4), at 272,367 to 274,829
_3614
EX28DRAFT_3615: EX28DRAFT_3615 - homoserine kinase (EC 2.7.1.39), at 274,831 to 275,760
_3615
EX28DRAFT_3616: EX28DRAFT_3616 - threonine synthase, at 275,764 to 277,050
_3616
EX28DRAFT_3617: EX28DRAFT_3617 - Protein of unknown function (DUF2502), at 277,308 to 277,610
_3617
EX28DRAFT_3618: EX28DRAFT_3618 - Uncharacterized protein conserved in bacteria, at 277,643 to 278,416
_3618
Position (kb) 275
276
277
278 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 274.820 kb on - strand at 275.019 kb on + strand, within EX28DRAFT_3615 at 275.683 kb on - strand at 275.719 kb on - strand at 275.758 kb on + strand at 275.758 kb on + strand at 275.762 kb on + strand at 275.763 kb on - strand at 275.763 kb on - strand at 275.774 kb on - strand at 275.774 kb on - strand at 275.774 kb on - strand at 275.782 kb on - strand at 275.790 kb on + strand at 276.200 kb on - strand, within EX28DRAFT_3616 at 276.276 kb on + strand, within EX28DRAFT_3616 at 276.437 kb on - strand, within EX28DRAFT_3616 at 276.807 kb on + strand, within EX28DRAFT_3616 at 276.808 kb on - strand, within EX28DRAFT_3616 at 276.808 kb on - strand, within EX28DRAFT_3616 at 276.920 kb on + strand, within EX28DRAFT_3616 at 277.087 kb on + strand at 277.112 kb on + strand at 277.200 kb on - strand at 277.267 kb on + strand at 277.313 kb on + strand at 277.393 kb on + strand, within EX28DRAFT_3617 at 277.394 kb on - strand, within EX28DRAFT_3617 at 277.394 kb on - strand, within EX28DRAFT_3617 at 277.403 kb on - strand, within EX28DRAFT_3617 at 277.428 kb on - strand, within EX28DRAFT_3617 at 277.488 kb on + strand, within EX28DRAFT_3617 at 277.489 kb on - strand, within EX28DRAFT_3617 at 277.489 kb on - strand, within EX28DRAFT_3617 at 277.644 kb on + strand at 277.704 kb on + strand at 277.890 kb on + strand, within EX28DRAFT_3618 at 277.890 kb on + strand, within EX28DRAFT_3618 at 277.890 kb on + strand, within EX28DRAFT_3618 at 277.891 kb on - strand, within EX28DRAFT_3618 at 277.891 kb on - strand, within EX28DRAFT_3618 at 277.891 kb on - strand, within EX28DRAFT_3618 at 277.891 kb on - strand, within EX28DRAFT_3618 at 277.893 kb on + strand, within EX28DRAFT_3618 at 277.894 kb on - strand, within EX28DRAFT_3618 at 277.894 kb on - strand, within EX28DRAFT_3618 at 277.986 kb on + strand, within EX28DRAFT_3618
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 274,820 - -2.5 275,019 + EX28DRAFT_3615 0.20 -3.5 275,683 - -2.6 275,719 - -3.2 275,758 + -4.0 275,758 + -3.3 275,762 + -3.1 275,763 - -4.4 275,763 - -3.4 275,774 - -4.1 275,774 - -2.1 275,774 - -2.6 275,782 - -3.0 275,790 + -3.6 276,200 - EX28DRAFT_3616 0.34 -3.6 276,276 + EX28DRAFT_3616 0.40 -2.4 276,437 - EX28DRAFT_3616 0.52 -5.6 276,807 + EX28DRAFT_3616 0.81 -3.0 276,808 - EX28DRAFT_3616 0.81 -3.9 276,808 - EX28DRAFT_3616 0.81 -4.5 276,920 + EX28DRAFT_3616 0.90 -1.7 277,087 + +1.0 277,112 + -0.2 277,200 - -1.0 277,267 + +0.2 277,313 + -0.5 277,393 + EX28DRAFT_3617 0.28 -0.7 277,394 - EX28DRAFT_3617 0.28 +2.5 277,394 - EX28DRAFT_3617 0.28 +0.0 277,403 - EX28DRAFT_3617 0.31 -0.0 277,428 - EX28DRAFT_3617 0.40 -0.1 277,488 + EX28DRAFT_3617 0.59 +0.3 277,489 - EX28DRAFT_3617 0.60 -0.3 277,489 - EX28DRAFT_3617 0.60 +3.4 277,644 + -0.6 277,704 + +0.1 277,890 + EX28DRAFT_3618 0.32 -0.2 277,890 + EX28DRAFT_3618 0.32 +0.0 277,890 + EX28DRAFT_3618 0.32 +0.8 277,891 - EX28DRAFT_3618 0.32 +0.2 277,891 - EX28DRAFT_3618 0.32 -1.4 277,891 - EX28DRAFT_3618 0.32 -0.6 277,891 - EX28DRAFT_3618 0.32 -0.7 277,893 + EX28DRAFT_3618 0.32 +0.3 277,894 - EX28DRAFT_3618 0.32 +0.1 277,894 - EX28DRAFT_3618 0.32 -0.6 277,986 + EX28DRAFT_3618 0.44 -0.5
Or see this region's nucleotide sequence