Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3544 and EX28DRAFT_3545 are separated by 9 nucleotides EX28DRAFT_3545 and EX28DRAFT_3546 are separated by 2 nucleotides EX28DRAFT_3546 and EX28DRAFT_3547 overlap by 4 nucleotides
EX28DRAFT_3544: EX28DRAFT_3544 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 208,288 to 208,668
_3544
EX28DRAFT_3545: EX28DRAFT_3545 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 208,678 to 209,529
_3545
EX28DRAFT_3546: EX28DRAFT_3546 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase, at 209,532 to 210,998
_3546
EX28DRAFT_3547: EX28DRAFT_3547 - 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase, C-terminal subunit/4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase, N-terminal subunit, at 210,995 to 212,272
_3547
Position (kb) 209
210
211 Strain fitness (log2 ratio) -2
-1
0
1
2 at 208.645 kb on - strand at 208.657 kb on - strand at 208.705 kb on - strand at 208.764 kb on - strand, within EX28DRAFT_3545 at 208.829 kb on + strand, within EX28DRAFT_3545 at 208.829 kb on + strand, within EX28DRAFT_3545 at 208.932 kb on - strand, within EX28DRAFT_3545 at 209.018 kb on + strand, within EX28DRAFT_3545 at 209.105 kb on - strand, within EX28DRAFT_3545 at 209.132 kb on + strand, within EX28DRAFT_3545 at 209.157 kb on - strand, within EX28DRAFT_3545 at 209.157 kb on - strand, within EX28DRAFT_3545 at 209.255 kb on + strand, within EX28DRAFT_3545 at 209.261 kb on + strand, within EX28DRAFT_3545 at 209.304 kb on - strand, within EX28DRAFT_3545 at 209.523 kb on + strand at 209.524 kb on - strand at 209.761 kb on + strand, within EX28DRAFT_3546 at 209.761 kb on + strand, within EX28DRAFT_3546 at 209.762 kb on - strand, within EX28DRAFT_3546 at 209.762 kb on - strand, within EX28DRAFT_3546 at 209.874 kb on - strand, within EX28DRAFT_3546 at 210.277 kb on + strand, within EX28DRAFT_3546 at 210.277 kb on + strand, within EX28DRAFT_3546 at 210.277 kb on + strand, within EX28DRAFT_3546 at 210.278 kb on - strand, within EX28DRAFT_3546 at 210.604 kb on + strand, within EX28DRAFT_3546 at 210.604 kb on + strand, within EX28DRAFT_3546 at 210.604 kb on + strand, within EX28DRAFT_3546 at 210.604 kb on + strand, within EX28DRAFT_3546 at 210.604 kb on + strand, within EX28DRAFT_3546 at 210.604 kb on + strand, within EX28DRAFT_3546 at 210.605 kb on - strand, within EX28DRAFT_3546 at 210.605 kb on - strand, within EX28DRAFT_3546 at 210.912 kb on + strand at 211.139 kb on - strand, within EX28DRAFT_3547 at 211.152 kb on + strand, within EX28DRAFT_3547 at 211.336 kb on - strand, within EX28DRAFT_3547 at 211.393 kb on - strand, within EX28DRAFT_3547 at 211.470 kb on + strand, within EX28DRAFT_3547 at 211.470 kb on + strand, within EX28DRAFT_3547 at 211.470 kb on + strand, within EX28DRAFT_3547 at 211.471 kb on - strand, within EX28DRAFT_3547 at 211.471 kb on - strand, within EX28DRAFT_3547 at 211.471 kb on - strand, within EX28DRAFT_3547 at 211.471 kb on - strand, within EX28DRAFT_3547 at 211.535 kb on - strand, within EX28DRAFT_3547 at 211.535 kb on - strand, within EX28DRAFT_3547 at 211.538 kb on + strand, within EX28DRAFT_3547 at 211.590 kb on + strand, within EX28DRAFT_3547 at 211.610 kb on + strand, within EX28DRAFT_3547 at 211.610 kb on + strand, within EX28DRAFT_3547
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 208,645 - -0.2 208,657 - -0.4 208,705 - +0.5 208,764 - EX28DRAFT_3545 0.10 -0.1 208,829 + EX28DRAFT_3545 0.18 -0.2 208,829 + EX28DRAFT_3545 0.18 -2.1 208,932 - EX28DRAFT_3545 0.30 +1.3 209,018 + EX28DRAFT_3545 0.40 +0.4 209,105 - EX28DRAFT_3545 0.50 -0.9 209,132 + EX28DRAFT_3545 0.53 +0.0 209,157 - EX28DRAFT_3545 0.56 -0.8 209,157 - EX28DRAFT_3545 0.56 -0.3 209,255 + EX28DRAFT_3545 0.68 -0.3 209,261 + EX28DRAFT_3545 0.68 -0.1 209,304 - EX28DRAFT_3545 0.73 +0.6 209,523 + -1.0 209,524 - +1.7 209,761 + EX28DRAFT_3546 0.16 +0.1 209,761 + EX28DRAFT_3546 0.16 -0.5 209,762 - EX28DRAFT_3546 0.16 -0.3 209,762 - EX28DRAFT_3546 0.16 +1.2 209,874 - EX28DRAFT_3546 0.23 +1.2 210,277 + EX28DRAFT_3546 0.51 +2.2 210,277 + EX28DRAFT_3546 0.51 -0.6 210,277 + EX28DRAFT_3546 0.51 -0.2 210,278 - EX28DRAFT_3546 0.51 +0.0 210,604 + EX28DRAFT_3546 0.73 +0.1 210,604 + EX28DRAFT_3546 0.73 +1.1 210,604 + EX28DRAFT_3546 0.73 +0.1 210,604 + EX28DRAFT_3546 0.73 +0.8 210,604 + EX28DRAFT_3546 0.73 -0.3 210,604 + EX28DRAFT_3546 0.73 +1.2 210,605 - EX28DRAFT_3546 0.73 +0.1 210,605 - EX28DRAFT_3546 0.73 +0.1 210,912 + +0.9 211,139 - EX28DRAFT_3547 0.11 -0.3 211,152 + EX28DRAFT_3547 0.12 +0.1 211,336 - EX28DRAFT_3547 0.27 +0.0 211,393 - EX28DRAFT_3547 0.31 -0.2 211,470 + EX28DRAFT_3547 0.37 +0.3 211,470 + EX28DRAFT_3547 0.37 +0.5 211,470 + EX28DRAFT_3547 0.37 +0.2 211,471 - EX28DRAFT_3547 0.37 +0.2 211,471 - EX28DRAFT_3547 0.37 +0.1 211,471 - EX28DRAFT_3547 0.37 +0.2 211,471 - EX28DRAFT_3547 0.37 -0.6 211,535 - EX28DRAFT_3547 0.42 -0.5 211,535 - EX28DRAFT_3547 0.42 -0.9 211,538 + EX28DRAFT_3547 0.42 -0.8 211,590 + EX28DRAFT_3547 0.47 +0.4 211,610 + EX28DRAFT_3547 0.48 -0.4 211,610 + EX28DRAFT_3547 0.48 -0.8
Or see this region's nucleotide sequence