Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3511 and EX28DRAFT_3512 are separated by 176 nucleotides EX28DRAFT_3512 and EX28DRAFT_3513 are separated by 84 nucleotides EX28DRAFT_3513 and EX28DRAFT_3514 are separated by 103 nucleotides EX28DRAFT_3514 and EX28DRAFT_3515 overlap by 14 nucleotides
EX28DRAFT_3511: EX28DRAFT_3511 - Transcriptional regulators containing a DNA-binding HTH domain and an aminotransferase domain (MocR family) and their eukaryotic orthologs, at 176,504 to 177,922
_3511
EX28DRAFT_3512: EX28DRAFT_3512 - Uncharacterized conserved small protein, at 178,099 to 178,260
_3512
EX28DRAFT_3513: EX28DRAFT_3513 - Transcriptional regulators containing a DNA-binding HTH domain and an aminotransferase domain (MocR family) and their eukaryotic orthologs, at 178,345 to 179,733
_3513
EX28DRAFT_3514: EX28DRAFT_3514 - alkylhydroperoxidase AhpD family core domain, at 179,837 to 180,316
_3514
EX28DRAFT_3515: EX28DRAFT_3515 - Transcriptional regulator, at 180,303 to 181,205
_3515
Position (kb)
178
179
180 Strain fitness (log2 ratio)
-2
-1
0
1 at 177.498 kb on + strand, within EX28DRAFT_3511 at 177.498 kb on + strand, within EX28DRAFT_3511 at 177.498 kb on + strand, within EX28DRAFT_3511 at 177.499 kb on - strand, within EX28DRAFT_3511 at 177.499 kb on - strand, within EX28DRAFT_3511 at 177.520 kb on + strand, within EX28DRAFT_3511 at 177.732 kb on + strand, within EX28DRAFT_3511 at 177.927 kb on + strand at 177.945 kb on + strand at 177.993 kb on + strand at 178.012 kb on + strand at 178.012 kb on + strand at 178.042 kb on + strand at 178.082 kb on - strand at 178.108 kb on + strand at 178.108 kb on + strand at 178.134 kb on + strand, within EX28DRAFT_3512 at 178.135 kb on - strand, within EX28DRAFT_3512 at 178.265 kb on - strand at 178.265 kb on - strand at 178.406 kb on + strand at 178.422 kb on + strand at 178.422 kb on + strand at 178.425 kb on - strand at 178.602 kb on - strand, within EX28DRAFT_3513 at 178.796 kb on + strand, within EX28DRAFT_3513 at 178.827 kb on + strand, within EX28DRAFT_3513 at 178.828 kb on - strand, within EX28DRAFT_3513 at 178.842 kb on + strand, within EX28DRAFT_3513 at 178.919 kb on + strand, within EX28DRAFT_3513 at 179.066 kb on + strand, within EX28DRAFT_3513 at 179.067 kb on - strand, within EX28DRAFT_3513 at 179.242 kb on + strand, within EX28DRAFT_3513 at 179.242 kb on + strand, within EX28DRAFT_3513 at 179.786 kb on + strand at 179.786 kb on + strand at 179.866 kb on + strand at 179.981 kb on - strand, within EX28DRAFT_3514 at 179.981 kb on - strand, within EX28DRAFT_3514 at 180.045 kb on - strand, within EX28DRAFT_3514 at 180.385 kb on + strand at 180.385 kb on + strand at 180.385 kb on + strand at 180.386 kb on - strand at 180.423 kb on + strand, within EX28DRAFT_3515 at 180.426 kb on + strand, within EX28DRAFT_3515 at 180.467 kb on - strand, within EX28DRAFT_3515 at 180.467 kb on - strand, within EX28DRAFT_3515 at 180.467 kb on - strand, within EX28DRAFT_3515 at 180.505 kb on - strand, within EX28DRAFT_3515 at 180.598 kb on + strand, within EX28DRAFT_3515 at 180.686 kb on - strand, within EX28DRAFT_3515
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 177,498 + EX28DRAFT_3511 0.70 +0.5 177,498 + EX28DRAFT_3511 0.70 +0.0 177,498 + EX28DRAFT_3511 0.70 +0.6 177,499 - EX28DRAFT_3511 0.70 -0.4 177,499 - EX28DRAFT_3511 0.70 +0.1 177,520 + EX28DRAFT_3511 0.72 +0.2 177,732 + EX28DRAFT_3511 0.87 -0.0 177,927 + -2.1 177,945 + -0.1 177,993 + +0.8 178,012 + +0.5 178,012 + -0.1 178,042 + -1.3 178,082 - +0.2 178,108 + -1.4 178,108 + -0.7 178,134 + EX28DRAFT_3512 0.22 +0.2 178,135 - EX28DRAFT_3512 0.22 -0.6 178,265 - -0.3 178,265 - +0.1 178,406 + -0.2 178,422 + -0.3 178,422 + +0.3 178,425 - -1.2 178,602 - EX28DRAFT_3513 0.19 -0.5 178,796 + EX28DRAFT_3513 0.32 -1.1 178,827 + EX28DRAFT_3513 0.35 -0.0 178,828 - EX28DRAFT_3513 0.35 +0.5 178,842 + EX28DRAFT_3513 0.36 -0.2 178,919 + EX28DRAFT_3513 0.41 -0.0 179,066 + EX28DRAFT_3513 0.52 +0.1 179,067 - EX28DRAFT_3513 0.52 -0.1 179,242 + EX28DRAFT_3513 0.65 -0.0 179,242 + EX28DRAFT_3513 0.65 +0.6 179,786 + +0.4 179,786 + -0.2 179,866 + -0.5 179,981 - EX28DRAFT_3514 0.30 +0.1 179,981 - EX28DRAFT_3514 0.30 +0.2 180,045 - EX28DRAFT_3514 0.43 +0.7 180,385 + +0.2 180,385 + -0.6 180,385 + -0.8 180,386 - +0.7 180,423 + EX28DRAFT_3515 0.13 +0.2 180,426 + EX28DRAFT_3515 0.14 -1.1 180,467 - EX28DRAFT_3515 0.18 +0.4 180,467 - EX28DRAFT_3515 0.18 +0.1 180,467 - EX28DRAFT_3515 0.18 +0.4 180,505 - EX28DRAFT_3515 0.22 -0.8 180,598 + EX28DRAFT_3515 0.33 -0.4 180,686 - EX28DRAFT_3515 0.42 -0.8
Or see this region's nucleotide sequence