Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3430 and EX28DRAFT_3431 overlap by 4 nucleotides EX28DRAFT_3431 and EX28DRAFT_3432 are separated by 83 nucleotides
EX28DRAFT_3430: EX28DRAFT_3430 - uncharacterized pyridoxal phosphate-dependent enzyme, at 99,394 to 100,512
_3430
EX28DRAFT_3431: EX28DRAFT_3431 - conserved hypothetical protein EF_0839/AHA_3917, at 100,509 to 101,249
_3431
EX28DRAFT_3432: EX28DRAFT_3432 - 3-carboxymuconate cyclase, at 101,333 to 102,469
_3432
Position (kb)
100
101
102 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 99.559 kb on + strand, within EX28DRAFT_3430 at 99.559 kb on + strand, within EX28DRAFT_3430 at 99.559 kb on + strand, within EX28DRAFT_3430 at 99.560 kb on - strand, within EX28DRAFT_3430 at 100.015 kb on + strand, within EX28DRAFT_3430 at 100.015 kb on + strand, within EX28DRAFT_3430 at 100.016 kb on - strand, within EX28DRAFT_3430 at 100.075 kb on + strand, within EX28DRAFT_3430 at 100.171 kb on + strand, within EX28DRAFT_3430 at 100.172 kb on - strand, within EX28DRAFT_3430 at 100.378 kb on + strand, within EX28DRAFT_3430 at 100.387 kb on + strand, within EX28DRAFT_3430 at 100.388 kb on - strand, within EX28DRAFT_3430 at 100.388 kb on - strand, within EX28DRAFT_3430 at 100.399 kb on + strand, within EX28DRAFT_3430 at 100.490 kb on + strand at 100.677 kb on + strand, within EX28DRAFT_3431 at 100.678 kb on - strand, within EX28DRAFT_3431 at 100.743 kb on - strand, within EX28DRAFT_3431 at 100.989 kb on + strand, within EX28DRAFT_3431 at 100.990 kb on - strand, within EX28DRAFT_3431 at 100.990 kb on - strand, within EX28DRAFT_3431 at 101.101 kb on - strand, within EX28DRAFT_3431 at 101.157 kb on - strand, within EX28DRAFT_3431 at 101.160 kb on + strand, within EX28DRAFT_3431 at 101.160 kb on + strand, within EX28DRAFT_3431 at 101.160 kb on + strand, within EX28DRAFT_3431 at 101.161 kb on - strand, within EX28DRAFT_3431 at 101.161 kb on - strand, within EX28DRAFT_3431 at 101.161 kb on - strand, within EX28DRAFT_3431 at 101.214 kb on - strand at 101.214 kb on - strand at 101.247 kb on + strand at 101.247 kb on + strand at 101.247 kb on + strand at 101.248 kb on - strand at 101.248 kb on - strand at 101.250 kb on + strand at 101.420 kb on + strand at 101.420 kb on + strand at 101.421 kb on - strand at 101.421 kb on - strand at 101.421 kb on - strand at 101.486 kb on - strand, within EX28DRAFT_3432 at 101.552 kb on + strand, within EX28DRAFT_3432 at 101.660 kb on + strand, within EX28DRAFT_3432 at 101.691 kb on + strand, within EX28DRAFT_3432 at 101.691 kb on + strand, within EX28DRAFT_3432 at 101.691 kb on + strand, within EX28DRAFT_3432 at 101.692 kb on - strand, within EX28DRAFT_3432 at 101.705 kb on + strand, within EX28DRAFT_3432 at 101.705 kb on + strand, within EX28DRAFT_3432 at 101.705 kb on + strand, within EX28DRAFT_3432 at 101.706 kb on - strand, within EX28DRAFT_3432 at 101.737 kb on + strand, within EX28DRAFT_3432 at 101.894 kb on + strand, within EX28DRAFT_3432 at 101.931 kb on - strand, within EX28DRAFT_3432 at 102.077 kb on - strand, within EX28DRAFT_3432 at 102.091 kb on + strand, within EX28DRAFT_3432 at 102.115 kb on - strand, within EX28DRAFT_3432 at 102.209 kb on + strand, within EX28DRAFT_3432 at 102.209 kb on + strand, within EX28DRAFT_3432 at 102.209 kb on + strand, within EX28DRAFT_3432 at 102.209 kb on + strand, within EX28DRAFT_3432 at 102.210 kb on - strand, within EX28DRAFT_3432 at 102.210 kb on - strand, within EX28DRAFT_3432 at 102.210 kb on - strand, within EX28DRAFT_3432 at 102.210 kb on - strand, within EX28DRAFT_3432
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 99,559 + EX28DRAFT_3430 0.15 +0.1 99,559 + EX28DRAFT_3430 0.15 +0.3 99,559 + EX28DRAFT_3430 0.15 +0.2 99,560 - EX28DRAFT_3430 0.15 -0.8 100,015 + EX28DRAFT_3430 0.55 +0.3 100,015 + EX28DRAFT_3430 0.55 +0.3 100,016 - EX28DRAFT_3430 0.56 -0.1 100,075 + EX28DRAFT_3430 0.61 -0.2 100,171 + EX28DRAFT_3430 0.69 +0.2 100,172 - EX28DRAFT_3430 0.70 +0.8 100,378 + EX28DRAFT_3430 0.88 -1.0 100,387 + EX28DRAFT_3430 0.89 -0.4 100,388 - EX28DRAFT_3430 0.89 +0.3 100,388 - EX28DRAFT_3430 0.89 -0.2 100,399 + EX28DRAFT_3430 0.90 +0.3 100,490 + -0.8 100,677 + EX28DRAFT_3431 0.23 +0.1 100,678 - EX28DRAFT_3431 0.23 +0.9 100,743 - EX28DRAFT_3431 0.32 -3.0 100,989 + EX28DRAFT_3431 0.65 +0.4 100,990 - EX28DRAFT_3431 0.65 -0.3 100,990 - EX28DRAFT_3431 0.65 -1.1 101,101 - EX28DRAFT_3431 0.80 -0.3 101,157 - EX28DRAFT_3431 0.87 -0.6 101,160 + EX28DRAFT_3431 0.88 +0.0 101,160 + EX28DRAFT_3431 0.88 -0.6 101,160 + EX28DRAFT_3431 0.88 +0.4 101,161 - EX28DRAFT_3431 0.88 +0.4 101,161 - EX28DRAFT_3431 0.88 -0.2 101,161 - EX28DRAFT_3431 0.88 -0.1 101,214 - -0.2 101,214 - -0.0 101,247 + +0.3 101,247 + +0.4 101,247 + -0.6 101,248 - -1.0 101,248 - +0.1 101,250 + +0.5 101,420 + -0.2 101,420 + -1.0 101,421 - -0.8 101,421 - -0.0 101,421 - +1.3 101,486 - EX28DRAFT_3432 0.13 -1.9 101,552 + EX28DRAFT_3432 0.19 +0.2 101,660 + EX28DRAFT_3432 0.29 +0.0 101,691 + EX28DRAFT_3432 0.31 -0.2 101,691 + EX28DRAFT_3432 0.31 +0.2 101,691 + EX28DRAFT_3432 0.31 -0.1 101,692 - EX28DRAFT_3432 0.32 +0.3 101,705 + EX28DRAFT_3432 0.33 -0.4 101,705 + EX28DRAFT_3432 0.33 -0.1 101,705 + EX28DRAFT_3432 0.33 -0.2 101,706 - EX28DRAFT_3432 0.33 -0.6 101,737 + EX28DRAFT_3432 0.36 +1.8 101,894 + EX28DRAFT_3432 0.49 +0.2 101,931 - EX28DRAFT_3432 0.53 -0.3 102,077 - EX28DRAFT_3432 0.65 -0.3 102,091 + EX28DRAFT_3432 0.67 -0.5 102,115 - EX28DRAFT_3432 0.69 -1.6 102,209 + EX28DRAFT_3432 0.77 -0.0 102,209 + EX28DRAFT_3432 0.77 +1.7 102,209 + EX28DRAFT_3432 0.77 +0.0 102,209 + EX28DRAFT_3432 0.77 -0.8 102,210 - EX28DRAFT_3432 0.77 -0.2 102,210 - EX28DRAFT_3432 0.77 -0.4 102,210 - EX28DRAFT_3432 0.77 +0.4 102,210 - EX28DRAFT_3432 0.77 +0.0
Or see this region's nucleotide sequence