Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3383 and EX28DRAFT_3384 are separated by 169 nucleotides EX28DRAFT_3384 and EX28DRAFT_3385 are separated by 341 nucleotides EX28DRAFT_3385 and EX28DRAFT_3386 are separated by 6 nucleotides
EX28DRAFT_3383: EX28DRAFT_3383 - Alpha/beta hydrolase family, at 53,319 to 54,041
_3383
EX28DRAFT_3384: EX28DRAFT_3384 - Protein of unknown function (DUF1471), at 54,211 to 54,486
_3384
EX28DRAFT_3385: EX28DRAFT_3385 - ribosomal protein S6, at 54,828 to 55,223
_3385
EX28DRAFT_3386: EX28DRAFT_3386 - restart primosome assembly protein PriB, at 55,230 to 55,544
_3386
Position (kb) 54
55 Strain fitness (log2 ratio) -2
-1
0
1
2 at 53.242 kb on - strand at 53.242 kb on - strand at 53.272 kb on - strand at 53.289 kb on + strand at 53.298 kb on + strand at 53.298 kb on + strand at 53.336 kb on - strand at 53.614 kb on - strand, within EX28DRAFT_3383 at 54.065 kb on - strand at 54.109 kb on - strand at 54.112 kb on - strand at 54.112 kb on - strand at 54.112 kb on - strand at 54.163 kb on + strand at 54.163 kb on + strand at 54.163 kb on + strand at 54.164 kb on - strand at 54.164 kb on - strand at 54.164 kb on - strand at 54.164 kb on - strand at 54.169 kb on + strand at 54.218 kb on + strand at 54.222 kb on + strand at 54.223 kb on - strand at 54.284 kb on + strand, within EX28DRAFT_3384 at 54.284 kb on + strand, within EX28DRAFT_3384 at 54.285 kb on - strand, within EX28DRAFT_3384 at 54.285 kb on - strand, within EX28DRAFT_3384 at 54.352 kb on - strand, within EX28DRAFT_3384 at 54.363 kb on - strand, within EX28DRAFT_3384 at 54.415 kb on + strand, within EX28DRAFT_3384 at 54.416 kb on - strand, within EX28DRAFT_3384 at 54.490 kb on - strand at 54.504 kb on + strand at 54.505 kb on - strand at 54.557 kb on - strand at 54.563 kb on + strand at 54.563 kb on + strand at 54.645 kb on - strand at 54.658 kb on - strand at 54.658 kb on - strand at 54.684 kb on + strand at 54.685 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 53,242 - +0.1 53,242 - +0.2 53,272 - +0.5 53,289 + +1.8 53,298 + +0.5 53,298 + -0.0 53,336 - -0.2 53,614 - EX28DRAFT_3383 0.41 +0.6 54,065 - -0.7 54,109 - -0.1 54,112 - +1.2 54,112 - +0.1 54,112 - +0.4 54,163 + -1.8 54,163 + +0.5 54,163 + -0.3 54,164 - -1.0 54,164 - -0.1 54,164 - +1.7 54,164 - -0.1 54,169 + -0.1 54,218 + +0.4 54,222 + +0.1 54,223 - -0.7 54,284 + EX28DRAFT_3384 0.26 -0.5 54,284 + EX28DRAFT_3384 0.26 +1.2 54,285 - EX28DRAFT_3384 0.27 -0.1 54,285 - EX28DRAFT_3384 0.27 -0.4 54,352 - EX28DRAFT_3384 0.51 -0.6 54,363 - EX28DRAFT_3384 0.55 -0.5 54,415 + EX28DRAFT_3384 0.74 -0.6 54,416 - EX28DRAFT_3384 0.74 -0.3 54,490 - -0.3 54,504 + +0.1 54,505 - -0.3 54,557 - +0.4 54,563 + +0.0 54,563 + +0.1 54,645 - -0.2 54,658 - -1.0 54,658 - -1.0 54,684 + -0.9 54,685 - +0.1
Or see this region's nucleotide sequence