Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3176 and EX28DRAFT_3177 are separated by 30 nucleotides EX28DRAFT_3177 and EX28DRAFT_3178 are separated by 9 nucleotides
EX28DRAFT_3176: EX28DRAFT_3176 - acetate kinase, at 476,655 to 477,863
_3176
EX28DRAFT_3177: EX28DRAFT_3177 - serine transporter, at 477,894 to 479,225
_3177
EX28DRAFT_3178: EX28DRAFT_3178 - Threonine dehydratase, at 479,235 to 480,224
_3178
Position (kb) 477
478
479
480 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 476.942 kb on + strand, within EX28DRAFT_3176 at 476.942 kb on + strand, within EX28DRAFT_3176 at 476.942 kb on + strand, within EX28DRAFT_3176 at 476.973 kb on + strand, within EX28DRAFT_3176 at 477.168 kb on + strand, within EX28DRAFT_3176 at 477.203 kb on + strand, within EX28DRAFT_3176 at 477.323 kb on - strand, within EX28DRAFT_3176 at 477.400 kb on + strand, within EX28DRAFT_3176 at 477.400 kb on + strand, within EX28DRAFT_3176 at 477.442 kb on - strand, within EX28DRAFT_3176 at 477.505 kb on + strand, within EX28DRAFT_3176 at 477.509 kb on + strand, within EX28DRAFT_3176 at 477.836 kb on + strand at 478.006 kb on + strand at 478.006 kb on + strand at 478.006 kb on + strand at 478.006 kb on + strand at 478.007 kb on - strand at 478.007 kb on - strand at 478.062 kb on - strand, within EX28DRAFT_3177 at 478.062 kb on - strand, within EX28DRAFT_3177 at 478.277 kb on - strand, within EX28DRAFT_3177 at 478.410 kb on - strand, within EX28DRAFT_3177 at 478.410 kb on - strand, within EX28DRAFT_3177 at 478.758 kb on - strand, within EX28DRAFT_3177 at 478.856 kb on + strand, within EX28DRAFT_3177 at 478.915 kb on + strand, within EX28DRAFT_3177 at 478.915 kb on + strand, within EX28DRAFT_3177 at 478.916 kb on - strand, within EX28DRAFT_3177 at 478.946 kb on + strand, within EX28DRAFT_3177 at 479.032 kb on + strand, within EX28DRAFT_3177 at 479.172 kb on - strand at 479.225 kb on + strand at 479.225 kb on + strand at 479.226 kb on - strand at 479.226 kb on - strand at 479.312 kb on + strand at 479.313 kb on - strand at 479.326 kb on + strand at 479.327 kb on - strand at 479.327 kb on - strand at 479.612 kb on + strand, within EX28DRAFT_3178 at 479.612 kb on + strand, within EX28DRAFT_3178 at 479.612 kb on + strand, within EX28DRAFT_3178 at 479.613 kb on - strand, within EX28DRAFT_3178 at 479.706 kb on + strand, within EX28DRAFT_3178 at 479.784 kb on - strand, within EX28DRAFT_3178 at 479.888 kb on + strand, within EX28DRAFT_3178 at 479.888 kb on + strand, within EX28DRAFT_3178 at 480.028 kb on - strand, within EX28DRAFT_3178 at 480.216 kb on - strand at 480.224 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 476,942 + EX28DRAFT_3176 0.24 +0.2 476,942 + EX28DRAFT_3176 0.24 +0.8 476,942 + EX28DRAFT_3176 0.24 +0.4 476,973 + EX28DRAFT_3176 0.26 +0.2 477,168 + EX28DRAFT_3176 0.42 +0.0 477,203 + EX28DRAFT_3176 0.45 -1.0 477,323 - EX28DRAFT_3176 0.55 +0.6 477,400 + EX28DRAFT_3176 0.62 +0.6 477,400 + EX28DRAFT_3176 0.62 -1.6 477,442 - EX28DRAFT_3176 0.65 -1.0 477,505 + EX28DRAFT_3176 0.70 +0.4 477,509 + EX28DRAFT_3176 0.71 -0.2 477,836 + -0.0 478,006 + +1.0 478,006 + -1.1 478,006 + -0.8 478,006 + +0.3 478,007 - +0.2 478,007 - -1.6 478,062 - EX28DRAFT_3177 0.13 +0.2 478,062 - EX28DRAFT_3177 0.13 -0.2 478,277 - EX28DRAFT_3177 0.29 -1.8 478,410 - EX28DRAFT_3177 0.39 -0.2 478,410 - EX28DRAFT_3177 0.39 -0.2 478,758 - EX28DRAFT_3177 0.65 -0.3 478,856 + EX28DRAFT_3177 0.72 -0.4 478,915 + EX28DRAFT_3177 0.77 +0.2 478,915 + EX28DRAFT_3177 0.77 -0.6 478,916 - EX28DRAFT_3177 0.77 -0.4 478,946 + EX28DRAFT_3177 0.79 -3.8 479,032 + EX28DRAFT_3177 0.85 -0.4 479,172 - +0.4 479,225 + +0.1 479,225 + +0.3 479,226 - +1.2 479,226 - -0.1 479,312 + -0.8 479,313 - +0.4 479,326 + +0.6 479,327 - -0.7 479,327 - +0.3 479,612 + EX28DRAFT_3178 0.38 +0.1 479,612 + EX28DRAFT_3178 0.38 -0.9 479,612 + EX28DRAFT_3178 0.38 +1.2 479,613 - EX28DRAFT_3178 0.38 +0.2 479,706 + EX28DRAFT_3178 0.48 -1.8 479,784 - EX28DRAFT_3178 0.55 +0.9 479,888 + EX28DRAFT_3178 0.66 -0.3 479,888 + EX28DRAFT_3178 0.66 -0.0 480,028 - EX28DRAFT_3178 0.80 -0.1 480,216 - +0.3 480,224 + -0.3
Or see this region's nucleotide sequence