Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3073 and EX28DRAFT_3074 are separated by 190 nucleotides EX28DRAFT_3074 and EX28DRAFT_3075 are separated by 2 nucleotides
EX28DRAFT_3073: EX28DRAFT_3073 - type I secretion outer membrane protein, TolC family, at 383,064 to 384,542
_3073
EX28DRAFT_3074: EX28DRAFT_3074 - Predicted integral membrane protein, at 384,733 to 385,401
_3074
EX28DRAFT_3075: EX28DRAFT_3075 - Glutathionylspermidine synthase, at 385,404 to 386,564
_3075
Position (kb) 384
385
386 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 383.910 kb on + strand, within EX28DRAFT_3073 at 384.039 kb on + strand, within EX28DRAFT_3073 at 384.221 kb on + strand, within EX28DRAFT_3073 at 384.221 kb on + strand, within EX28DRAFT_3073 at 384.222 kb on - strand, within EX28DRAFT_3073 at 384.258 kb on + strand, within EX28DRAFT_3073 at 384.259 kb on - strand, within EX28DRAFT_3073 at 384.444 kb on + strand at 384.447 kb on - strand at 384.525 kb on - strand at 384.537 kb on - strand at 384.540 kb on + strand at 384.639 kb on + strand at 384.639 kb on + strand at 384.705 kb on + strand at 384.750 kb on + strand at 384.829 kb on - strand, within EX28DRAFT_3074 at 384.829 kb on - strand, within EX28DRAFT_3074 at 384.829 kb on - strand, within EX28DRAFT_3074 at 384.961 kb on - strand, within EX28DRAFT_3074 at 384.961 kb on - strand, within EX28DRAFT_3074 at 384.973 kb on + strand, within EX28DRAFT_3074 at 384.973 kb on + strand, within EX28DRAFT_3074 at 385.111 kb on + strand, within EX28DRAFT_3074 at 385.112 kb on - strand, within EX28DRAFT_3074 at 385.205 kb on - strand, within EX28DRAFT_3074 at 385.206 kb on + strand, within EX28DRAFT_3074 at 385.207 kb on - strand, within EX28DRAFT_3074 at 385.466 kb on + strand at 385.482 kb on + strand at 385.515 kb on + strand at 385.515 kb on + strand at 385.515 kb on + strand at 385.516 kb on - strand at 385.516 kb on - strand at 385.695 kb on + strand, within EX28DRAFT_3075 at 385.696 kb on - strand, within EX28DRAFT_3075 at 385.751 kb on + strand, within EX28DRAFT_3075 at 385.974 kb on + strand, within EX28DRAFT_3075 at 386.022 kb on + strand, within EX28DRAFT_3075 at 386.116 kb on - strand, within EX28DRAFT_3075 at 386.116 kb on - strand, within EX28DRAFT_3075 at 386.116 kb on - strand, within EX28DRAFT_3075 at 386.116 kb on - strand, within EX28DRAFT_3075 at 386.198 kb on + strand, within EX28DRAFT_3075 at 386.262 kb on + strand, within EX28DRAFT_3075 at 386.298 kb on + strand, within EX28DRAFT_3075 at 386.299 kb on - strand, within EX28DRAFT_3075 at 386.299 kb on - strand, within EX28DRAFT_3075 at 386.299 kb on - strand, within EX28DRAFT_3075 at 386.299 kb on - strand, within EX28DRAFT_3075
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 383,910 + EX28DRAFT_3073 0.57 -0.6 384,039 + EX28DRAFT_3073 0.66 -1.3 384,221 + EX28DRAFT_3073 0.78 -0.9 384,221 + EX28DRAFT_3073 0.78 -0.4 384,222 - EX28DRAFT_3073 0.78 +0.7 384,258 + EX28DRAFT_3073 0.81 +0.8 384,259 - EX28DRAFT_3073 0.81 +0.9 384,444 + +0.2 384,447 - +0.9 384,525 - -0.1 384,537 - +0.1 384,540 + +1.2 384,639 + +0.4 384,639 + +0.3 384,705 + +0.1 384,750 + -1.0 384,829 - EX28DRAFT_3074 0.14 +0.2 384,829 - EX28DRAFT_3074 0.14 -2.1 384,829 - EX28DRAFT_3074 0.14 +0.5 384,961 - EX28DRAFT_3074 0.34 +0.4 384,961 - EX28DRAFT_3074 0.34 +2.8 384,973 + EX28DRAFT_3074 0.36 +1.5 384,973 + EX28DRAFT_3074 0.36 +0.4 385,111 + EX28DRAFT_3074 0.57 +0.3 385,112 - EX28DRAFT_3074 0.57 +0.0 385,205 - EX28DRAFT_3074 0.71 +0.4 385,206 + EX28DRAFT_3074 0.71 +0.2 385,207 - EX28DRAFT_3074 0.71 +0.1 385,466 + -0.9 385,482 + -0.8 385,515 + +0.5 385,515 + -0.2 385,515 + -0.4 385,516 - +0.5 385,516 - -0.5 385,695 + EX28DRAFT_3075 0.25 -0.3 385,696 - EX28DRAFT_3075 0.25 +1.8 385,751 + EX28DRAFT_3075 0.30 +0.1 385,974 + EX28DRAFT_3075 0.49 +0.2 386,022 + EX28DRAFT_3075 0.53 -1.0 386,116 - EX28DRAFT_3075 0.61 +0.6 386,116 - EX28DRAFT_3075 0.61 +0.2 386,116 - EX28DRAFT_3075 0.61 -3.3 386,116 - EX28DRAFT_3075 0.61 -0.1 386,198 + EX28DRAFT_3075 0.68 +0.1 386,262 + EX28DRAFT_3075 0.74 +0.0 386,298 + EX28DRAFT_3075 0.77 -0.5 386,299 - EX28DRAFT_3075 0.77 +0.1 386,299 - EX28DRAFT_3075 0.77 +0.4 386,299 - EX28DRAFT_3075 0.77 +0.2 386,299 - EX28DRAFT_3075 0.77 +0.0
Or see this region's nucleotide sequence