Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3044 and EX28DRAFT_3045 are separated by 4 nucleotides EX28DRAFT_3045 and EX28DRAFT_3046 are separated by 251 nucleotides EX28DRAFT_3046 and EX28DRAFT_3047 are separated by 143 nucleotides EX28DRAFT_3047 and EX28DRAFT_3048 are separated by 39 nucleotides
EX28DRAFT_3044: EX28DRAFT_3044 - TonB system transport protein ExbD, group 1, at 351,645 to 352,070
_3044
EX28DRAFT_3045: EX28DRAFT_3045 - tonB-system energizer ExbB, at 352,075 to 352,806
_3045
EX28DRAFT_3046: EX28DRAFT_3046 - cystathionine beta-lyase, bacterial, at 353,058 to 354,245
_3046
EX28DRAFT_3047: EX28DRAFT_3047 - Uncharacterized membrane-associated protein, at 354,389 to 355,048
_3047
EX28DRAFT_3048: EX28DRAFT_3048 - AraC-type DNA-binding domain-containing proteins, at 355,088 to 355,987
_3048
Position (kb) 353
354
355 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 352.071 kb on - strand at 352.071 kb on - strand at 352.093 kb on - strand at 352.223 kb on + strand, within EX28DRAFT_3045 at 352.223 kb on + strand, within EX28DRAFT_3045 at 352.223 kb on + strand, within EX28DRAFT_3045 at 352.223 kb on + strand, within EX28DRAFT_3045 at 352.224 kb on - strand, within EX28DRAFT_3045 at 352.642 kb on - strand, within EX28DRAFT_3045 at 352.760 kb on + strand at 352.760 kb on + strand at 352.761 kb on - strand at 352.761 kb on - strand at 352.844 kb on + strand at 352.844 kb on + strand at 352.845 kb on - strand at 352.845 kb on - strand at 352.845 kb on - strand at 352.847 kb on + strand at 352.847 kb on + strand at 352.848 kb on - strand at 352.885 kb on + strand at 352.889 kb on - strand at 352.977 kb on + strand at 353.037 kb on - strand at 353.366 kb on + strand, within EX28DRAFT_3046 at 353.431 kb on + strand, within EX28DRAFT_3046 at 353.486 kb on + strand, within EX28DRAFT_3046 at 353.663 kb on + strand, within EX28DRAFT_3046 at 353.663 kb on + strand, within EX28DRAFT_3046 at 353.688 kb on + strand, within EX28DRAFT_3046 at 353.805 kb on + strand, within EX28DRAFT_3046 at 353.805 kb on + strand, within EX28DRAFT_3046 at 353.806 kb on - strand, within EX28DRAFT_3046 at 353.806 kb on - strand, within EX28DRAFT_3046 at 353.940 kb on - strand, within EX28DRAFT_3046 at 354.045 kb on - strand, within EX28DRAFT_3046 at 354.051 kb on - strand, within EX28DRAFT_3046 at 354.070 kb on - strand, within EX28DRAFT_3046 at 354.362 kb on + strand at 354.362 kb on + strand at 354.363 kb on - strand at 354.364 kb on + strand at 354.364 kb on + strand at 354.364 kb on + strand at 354.365 kb on - strand at 354.365 kb on - strand at 354.365 kb on - strand at 354.365 kb on - strand at 354.406 kb on + strand at 354.647 kb on + strand, within EX28DRAFT_3047 at 354.647 kb on + strand, within EX28DRAFT_3047 at 354.716 kb on + strand, within EX28DRAFT_3047 at 354.717 kb on - strand, within EX28DRAFT_3047 at 354.717 kb on - strand, within EX28DRAFT_3047 at 354.917 kb on - strand, within EX28DRAFT_3047 at 355.035 kb on - strand at 355.089 kb on + strand at 355.089 kb on + strand at 355.146 kb on + strand at 355.147 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 352,071 - +0.2 352,071 - +0.2 352,093 - -0.7 352,223 + EX28DRAFT_3045 0.20 -1.6 352,223 + EX28DRAFT_3045 0.20 -2.1 352,223 + EX28DRAFT_3045 0.20 -1.2 352,223 + EX28DRAFT_3045 0.20 -2.2 352,224 - EX28DRAFT_3045 0.20 -0.1 352,642 - EX28DRAFT_3045 0.77 -0.1 352,760 + -0.9 352,760 + -0.3 352,761 - -0.4 352,761 - -0.5 352,844 + -1.4 352,844 + -1.1 352,845 - +0.2 352,845 - -1.6 352,845 - +0.5 352,847 + -0.2 352,847 + +0.1 352,848 - +0.2 352,885 + +0.2 352,889 - +0.6 352,977 + -0.9 353,037 - -2.4 353,366 + EX28DRAFT_3046 0.26 -5.0 353,431 + EX28DRAFT_3046 0.31 -2.8 353,486 + EX28DRAFT_3046 0.36 -3.4 353,663 + EX28DRAFT_3046 0.51 -1.9 353,663 + EX28DRAFT_3046 0.51 -5.3 353,688 + EX28DRAFT_3046 0.53 -2.4 353,805 + EX28DRAFT_3046 0.63 -3.3 353,805 + EX28DRAFT_3046 0.63 -4.0 353,806 - EX28DRAFT_3046 0.63 -4.6 353,806 - EX28DRAFT_3046 0.63 -4.3 353,940 - EX28DRAFT_3046 0.74 -3.9 354,045 - EX28DRAFT_3046 0.83 -3.5 354,051 - EX28DRAFT_3046 0.84 -4.6 354,070 - EX28DRAFT_3046 0.85 -2.4 354,362 + +0.5 354,362 + -0.2 354,363 - -0.0 354,364 + -1.0 354,364 + +0.4 354,364 + +0.0 354,365 - +0.6 354,365 - +2.8 354,365 - -0.2 354,365 - -0.8 354,406 + +0.1 354,647 + EX28DRAFT_3047 0.39 +1.3 354,647 + EX28DRAFT_3047 0.39 -0.4 354,716 + EX28DRAFT_3047 0.50 +0.4 354,717 - EX28DRAFT_3047 0.50 +0.5 354,717 - EX28DRAFT_3047 0.50 -0.8 354,917 - EX28DRAFT_3047 0.80 -0.6 355,035 - -1.8 355,089 + +0.2 355,089 + -0.1 355,146 + -0.2 355,147 - +0.1
Or see this region's nucleotide sequence