Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3013 and EX28DRAFT_3014 are separated by 95 nucleotides EX28DRAFT_3014 and EX28DRAFT_3015 are separated by 139 nucleotides EX28DRAFT_3015 and EX28DRAFT_3016 are separated by 94 nucleotides EX28DRAFT_3016 and EX28DRAFT_3017 are separated by 170 nucleotides
EX28DRAFT_3013: EX28DRAFT_3013 - Uncharacterized membrane protein, possible Na+ channel or pump, at 322,967 to 323,683
_3013
EX28DRAFT_3014: EX28DRAFT_3014 - tRNA, at 323,779 to 323,854
_3014
EX28DRAFT_3015: EX28DRAFT_3015 - hypothetical protein, at 323,994 to 324,386
_3015
EX28DRAFT_3016: EX28DRAFT_3016 - Protein of unknown function (DUF1493), at 324,481 to 324,861
_3016
EX28DRAFT_3017: EX28DRAFT_3017 - Bacterial regulatory proteins, luxR family, at 325,032 to 325,610
_3017
Position (kb) 323
324
325 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 323.104 kb on + strand, within EX28DRAFT_3013 at 323.112 kb on + strand, within EX28DRAFT_3013 at 323.203 kb on + strand, within EX28DRAFT_3013 at 323.204 kb on - strand, within EX28DRAFT_3013 at 323.204 kb on - strand, within EX28DRAFT_3013 at 323.281 kb on + strand, within EX28DRAFT_3013 at 323.291 kb on + strand, within EX28DRAFT_3013 at 323.599 kb on + strand, within EX28DRAFT_3013 at 323.891 kb on + strand at 323.891 kb on + strand at 323.893 kb on + strand at 323.950 kb on - strand at 323.970 kb on + strand at 323.970 kb on + strand at 323.970 kb on + strand at 323.970 kb on + strand at 324.000 kb on - strand at 324.000 kb on - strand at 324.000 kb on - strand at 324.089 kb on + strand, within EX28DRAFT_3015 at 324.089 kb on + strand, within EX28DRAFT_3015 at 324.174 kb on - strand, within EX28DRAFT_3015 at 324.174 kb on - strand, within EX28DRAFT_3015 at 324.174 kb on - strand, within EX28DRAFT_3015 at 324.182 kb on + strand, within EX28DRAFT_3015 at 324.259 kb on + strand, within EX28DRAFT_3015 at 324.259 kb on + strand, within EX28DRAFT_3015 at 324.376 kb on + strand at 324.377 kb on - strand at 324.381 kb on - strand at 324.402 kb on - strand at 324.438 kb on - strand at 324.454 kb on - strand at 324.509 kb on - strand at 324.526 kb on - strand, within EX28DRAFT_3016 at 324.528 kb on - strand, within EX28DRAFT_3016 at 324.528 kb on - strand, within EX28DRAFT_3016 at 324.585 kb on + strand, within EX28DRAFT_3016 at 324.585 kb on + strand, within EX28DRAFT_3016 at 324.585 kb on + strand, within EX28DRAFT_3016 at 324.585 kb on + strand, within EX28DRAFT_3016 at 324.585 kb on + strand, within EX28DRAFT_3016 at 324.586 kb on - strand, within EX28DRAFT_3016 at 324.586 kb on - strand, within EX28DRAFT_3016 at 324.612 kb on - strand, within EX28DRAFT_3016 at 324.674 kb on + strand, within EX28DRAFT_3016 at 324.674 kb on + strand, within EX28DRAFT_3016 at 324.755 kb on - strand, within EX28DRAFT_3016 at 324.805 kb on - strand, within EX28DRAFT_3016 at 324.836 kb on - strand at 324.857 kb on - strand at 324.857 kb on - strand at 324.901 kb on - strand at 324.901 kb on - strand at 324.941 kb on - strand at 324.990 kb on - strand at 325.009 kb on + strand at 325.009 kb on + strand at 325.009 kb on + strand at 325.010 kb on - strand at 325.010 kb on - strand at 325.236 kb on - strand, within EX28DRAFT_3017 at 325.248 kb on - strand, within EX28DRAFT_3017 at 325.303 kb on - strand, within EX28DRAFT_3017 at 325.344 kb on + strand, within EX28DRAFT_3017 at 325.344 kb on + strand, within EX28DRAFT_3017 at 325.345 kb on - strand, within EX28DRAFT_3017 at 325.352 kb on + strand, within EX28DRAFT_3017
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 323,104 + EX28DRAFT_3013 0.19 -0.6 323,112 + EX28DRAFT_3013 0.20 -0.0 323,203 + EX28DRAFT_3013 0.33 -0.8 323,204 - EX28DRAFT_3013 0.33 -1.9 323,204 - EX28DRAFT_3013 0.33 +0.0 323,281 + EX28DRAFT_3013 0.44 -0.3 323,291 + EX28DRAFT_3013 0.45 +0.0 323,599 + EX28DRAFT_3013 0.88 -0.8 323,891 + +0.7 323,891 + +0.0 323,893 + +0.4 323,950 - +2.5 323,970 + -0.5 323,970 + +1.6 323,970 + -0.2 323,970 + -0.1 324,000 - +0.0 324,000 - +0.1 324,000 - -0.6 324,089 + EX28DRAFT_3015 0.24 +0.0 324,089 + EX28DRAFT_3015 0.24 -0.7 324,174 - EX28DRAFT_3015 0.46 -1.5 324,174 - EX28DRAFT_3015 0.46 -0.6 324,174 - EX28DRAFT_3015 0.46 -0.3 324,182 + EX28DRAFT_3015 0.48 -0.0 324,259 + EX28DRAFT_3015 0.67 +0.6 324,259 + EX28DRAFT_3015 0.67 -1.1 324,376 + +0.1 324,377 - -0.2 324,381 - -0.6 324,402 - -0.2 324,438 - -0.2 324,454 - +0.5 324,509 - +0.5 324,526 - EX28DRAFT_3016 0.12 +0.4 324,528 - EX28DRAFT_3016 0.12 +0.2 324,528 - EX28DRAFT_3016 0.12 -0.2 324,585 + EX28DRAFT_3016 0.27 +0.2 324,585 + EX28DRAFT_3016 0.27 -0.0 324,585 + EX28DRAFT_3016 0.27 -0.2 324,585 + EX28DRAFT_3016 0.27 -0.3 324,585 + EX28DRAFT_3016 0.27 -0.8 324,586 - EX28DRAFT_3016 0.28 +0.3 324,586 - EX28DRAFT_3016 0.28 -0.2 324,612 - EX28DRAFT_3016 0.34 -0.1 324,674 + EX28DRAFT_3016 0.51 -0.6 324,674 + EX28DRAFT_3016 0.51 -0.6 324,755 - EX28DRAFT_3016 0.72 +0.5 324,805 - EX28DRAFT_3016 0.85 -0.0 324,836 - +0.9 324,857 - +0.2 324,857 - +0.5 324,901 - +0.2 324,901 - -2.9 324,941 - +0.5 324,990 - -0.6 325,009 + +0.3 325,009 + -0.1 325,009 + -1.0 325,010 - -0.2 325,010 - -0.0 325,236 - EX28DRAFT_3017 0.35 +0.1 325,248 - EX28DRAFT_3017 0.37 -0.0 325,303 - EX28DRAFT_3017 0.47 +0.4 325,344 + EX28DRAFT_3017 0.54 +0.2 325,344 + EX28DRAFT_3017 0.54 -0.4 325,345 - EX28DRAFT_3017 0.54 -1.0 325,352 + EX28DRAFT_3017 0.55 +0.2
Or see this region's nucleotide sequence