Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3007 and EX28DRAFT_3008 are separated by 13 nucleotides EX28DRAFT_3008 and EX28DRAFT_3009 are separated by 17 nucleotides EX28DRAFT_3009 and EX28DRAFT_3010 are separated by 345 nucleotides
EX28DRAFT_3007: EX28DRAFT_3007 - [citrate (pro-3S)-lyase] ligase, at 314,664 to 315,686
_3007
EX28DRAFT_3008: EX28DRAFT_3008 - 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway), at 315,700 to 316,563
_3008
EX28DRAFT_3009: EX28DRAFT_3009 - Na+/citrate symporter, at 316,581 to 317,942
_3009
EX28DRAFT_3010: EX28DRAFT_3010 - Signal transduction histidine kinase regulating citrate/malate metabolism, at 318,288 to 319,913
_3010
Position (kb) 316
317
318 Strain fitness (log2 ratio) -1
0
1 at 315.697 kb on + strand at 315.701 kb on + strand at 315.701 kb on + strand at 315.701 kb on + strand at 315.702 kb on - strand at 315.802 kb on - strand, within EX28DRAFT_3008 at 315.802 kb on - strand, within EX28DRAFT_3008 at 315.802 kb on - strand, within EX28DRAFT_3008 at 315.894 kb on - strand, within EX28DRAFT_3008 at 315.894 kb on - strand, within EX28DRAFT_3008 at 316.104 kb on - strand, within EX28DRAFT_3008 at 316.319 kb on + strand, within EX28DRAFT_3008 at 316.319 kb on + strand, within EX28DRAFT_3008 at 316.319 kb on + strand, within EX28DRAFT_3008 at 316.367 kb on - strand, within EX28DRAFT_3008 at 316.490 kb on - strand at 316.766 kb on + strand, within EX28DRAFT_3009 at 316.766 kb on + strand, within EX28DRAFT_3009 at 317.144 kb on + strand, within EX28DRAFT_3009 at 317.551 kb on + strand, within EX28DRAFT_3009 at 317.552 kb on - strand, within EX28DRAFT_3009 at 317.558 kb on - strand, within EX28DRAFT_3009 at 317.558 kb on - strand, within EX28DRAFT_3009 at 317.627 kb on - strand, within EX28DRAFT_3009 at 317.790 kb on - strand, within EX28DRAFT_3009 at 317.831 kb on + strand at 317.859 kb on - strand at 317.943 kb on - strand at 318.133 kb on - strand at 318.309 kb on - strand at 318.539 kb on + strand, within EX28DRAFT_3010 at 318.539 kb on + strand, within EX28DRAFT_3010 at 318.557 kb on + strand, within EX28DRAFT_3010 at 318.635 kb on - strand, within EX28DRAFT_3010 at 318.751 kb on - strand, within EX28DRAFT_3010 at 318.762 kb on + strand, within EX28DRAFT_3010 at 318.762 kb on + strand, within EX28DRAFT_3010 at 318.797 kb on + strand, within EX28DRAFT_3010 at 318.837 kb on - strand, within EX28DRAFT_3010 at 318.837 kb on - strand, within EX28DRAFT_3010 at 318.863 kb on + strand, within EX28DRAFT_3010 at 318.941 kb on + strand, within EX28DRAFT_3010
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 315,697 + -0.5 315,701 + +0.9 315,701 + +0.1 315,701 + -0.4 315,702 - +0.7 315,802 - EX28DRAFT_3008 0.12 -0.4 315,802 - EX28DRAFT_3008 0.12 -0.3 315,802 - EX28DRAFT_3008 0.12 -0.2 315,894 - EX28DRAFT_3008 0.22 -0.9 315,894 - EX28DRAFT_3008 0.22 -0.1 316,104 - EX28DRAFT_3008 0.47 +0.1 316,319 + EX28DRAFT_3008 0.72 -0.3 316,319 + EX28DRAFT_3008 0.72 +0.1 316,319 + EX28DRAFT_3008 0.72 -0.6 316,367 - EX28DRAFT_3008 0.77 +0.8 316,490 - +1.3 316,766 + EX28DRAFT_3009 0.14 +0.5 316,766 + EX28DRAFT_3009 0.14 +0.5 317,144 + EX28DRAFT_3009 0.41 -0.1 317,551 + EX28DRAFT_3009 0.71 +0.2 317,552 - EX28DRAFT_3009 0.71 +0.6 317,558 - EX28DRAFT_3009 0.72 -1.1 317,558 - EX28DRAFT_3009 0.72 +0.3 317,627 - EX28DRAFT_3009 0.77 -0.6 317,790 - EX28DRAFT_3009 0.89 +0.6 317,831 + +0.5 317,859 - -0.4 317,943 - +1.1 318,133 - -0.3 318,309 - -0.0 318,539 + EX28DRAFT_3010 0.15 +0.1 318,539 + EX28DRAFT_3010 0.15 +0.1 318,557 + EX28DRAFT_3010 0.17 +0.1 318,635 - EX28DRAFT_3010 0.21 -0.6 318,751 - EX28DRAFT_3010 0.28 -0.5 318,762 + EX28DRAFT_3010 0.29 -0.0 318,762 + EX28DRAFT_3010 0.29 +0.5 318,797 + EX28DRAFT_3010 0.31 -0.7 318,837 - EX28DRAFT_3010 0.34 -1.4 318,837 - EX28DRAFT_3010 0.34 +0.0 318,863 + EX28DRAFT_3010 0.35 +0.1 318,941 + EX28DRAFT_3010 0.40 -1.3
Or see this region's nucleotide sequence