Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2949 and EX28DRAFT_2950 are separated by 110 nucleotides EX28DRAFT_2950 and EX28DRAFT_2951 are separated by 165 nucleotides EX28DRAFT_2951 and EX28DRAFT_2952 are separated by 51 nucleotides
EX28DRAFT_2949: EX28DRAFT_2949 - folate-binding protein YgfZ, at 258,486 to 259,466
_2949
EX28DRAFT_2950: EX28DRAFT_2950 - channel protein, hemolysin III family, at 259,577 to 260,236
_2950
EX28DRAFT_2951: EX28DRAFT_2951 - Uncharacterized protein conserved in bacteria, at 260,402 to 260,713
_2951
EX28DRAFT_2952: EX28DRAFT_2952 - transcriptional regulator, RpiR family, at 260,765 to 261,496
_2952
Position (kb) 259
260
261 Strain fitness (log2 ratio) -2
-1
0
1
2 at 259.472 kb on + strand at 259.472 kb on + strand at 259.472 kb on + strand at 259.511 kb on + strand at 259.593 kb on + strand at 259.599 kb on + strand at 259.599 kb on + strand at 259.600 kb on - strand at 259.600 kb on - strand at 259.662 kb on + strand, within EX28DRAFT_2950 at 259.705 kb on - strand, within EX28DRAFT_2950 at 259.718 kb on + strand, within EX28DRAFT_2950 at 259.767 kb on + strand, within EX28DRAFT_2950 at 259.768 kb on - strand, within EX28DRAFT_2950 at 259.803 kb on + strand, within EX28DRAFT_2950 at 259.803 kb on + strand, within EX28DRAFT_2950 at 259.849 kb on + strand, within EX28DRAFT_2950 at 259.849 kb on + strand, within EX28DRAFT_2950 at 259.941 kb on + strand, within EX28DRAFT_2950 at 259.941 kb on + strand, within EX28DRAFT_2950 at 259.941 kb on + strand, within EX28DRAFT_2950 at 259.942 kb on - strand, within EX28DRAFT_2950 at 259.942 kb on - strand, within EX28DRAFT_2950 at 259.962 kb on + strand, within EX28DRAFT_2950 at 259.963 kb on - strand, within EX28DRAFT_2950 at 259.963 kb on - strand, within EX28DRAFT_2950 at 259.966 kb on + strand, within EX28DRAFT_2950 at 259.966 kb on + strand, within EX28DRAFT_2950 at 260.057 kb on - strand, within EX28DRAFT_2950 at 260.076 kb on + strand, within EX28DRAFT_2950 at 260.129 kb on + strand, within EX28DRAFT_2950 at 260.276 kb on + strand at 260.276 kb on + strand at 260.280 kb on + strand at 260.280 kb on + strand at 260.280 kb on + strand at 260.280 kb on + strand at 260.281 kb on - strand at 260.281 kb on - strand at 260.281 kb on - strand at 260.286 kb on - strand at 260.351 kb on - strand at 260.364 kb on + strand at 260.477 kb on - strand, within EX28DRAFT_2951 at 260.477 kb on - strand, within EX28DRAFT_2951 at 260.568 kb on + strand, within EX28DRAFT_2951 at 260.717 kb on - strand at 260.752 kb on - strand at 260.822 kb on + strand at 260.822 kb on + strand at 260.822 kb on + strand at 260.822 kb on + strand at 260.823 kb on - strand at 260.823 kb on - strand at 260.823 kb on - strand at 260.823 kb on - strand at 260.852 kb on + strand, within EX28DRAFT_2952 at 260.852 kb on + strand, within EX28DRAFT_2952 at 260.852 kb on + strand, within EX28DRAFT_2952 at 260.853 kb on - strand, within EX28DRAFT_2952 at 260.853 kb on - strand, within EX28DRAFT_2952 at 260.853 kb on - strand, within EX28DRAFT_2952 at 260.853 kb on - strand, within EX28DRAFT_2952 at 260.927 kb on - strand, within EX28DRAFT_2952 at 261.066 kb on + strand, within EX28DRAFT_2952 at 261.082 kb on + strand, within EX28DRAFT_2952 at 261.106 kb on + strand, within EX28DRAFT_2952 at 261.108 kb on + strand, within EX28DRAFT_2952 at 261.203 kb on + strand, within EX28DRAFT_2952
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 259,472 + +0.5 259,472 + +0.5 259,472 + +0.2 259,511 + +0.2 259,593 + -0.3 259,599 + +1.2 259,599 + -1.2 259,600 - +0.3 259,600 - -2.1 259,662 + EX28DRAFT_2950 0.13 +0.8 259,705 - EX28DRAFT_2950 0.19 +0.6 259,718 + EX28DRAFT_2950 0.21 -0.1 259,767 + EX28DRAFT_2950 0.29 +0.4 259,768 - EX28DRAFT_2950 0.29 +0.9 259,803 + EX28DRAFT_2950 0.34 +1.0 259,803 + EX28DRAFT_2950 0.34 -0.3 259,849 + EX28DRAFT_2950 0.41 -0.2 259,849 + EX28DRAFT_2950 0.41 +0.4 259,941 + EX28DRAFT_2950 0.55 +0.2 259,941 + EX28DRAFT_2950 0.55 +0.9 259,941 + EX28DRAFT_2950 0.55 +0.4 259,942 - EX28DRAFT_2950 0.55 -0.4 259,942 - EX28DRAFT_2950 0.55 +0.2 259,962 + EX28DRAFT_2950 0.58 +0.8 259,963 - EX28DRAFT_2950 0.58 +0.9 259,963 - EX28DRAFT_2950 0.58 +0.2 259,966 + EX28DRAFT_2950 0.59 +0.2 259,966 + EX28DRAFT_2950 0.59 -0.3 260,057 - EX28DRAFT_2950 0.73 +0.2 260,076 + EX28DRAFT_2950 0.76 -0.3 260,129 + EX28DRAFT_2950 0.84 +0.6 260,276 + -0.3 260,276 + +0.1 260,280 + +1.0 260,280 + -1.5 260,280 + -0.0 260,280 + -0.1 260,281 - +0.7 260,281 - +0.6 260,281 - +0.2 260,286 - -0.5 260,351 - -0.2 260,364 + +0.4 260,477 - EX28DRAFT_2951 0.24 -2.1 260,477 - EX28DRAFT_2951 0.24 -0.3 260,568 + EX28DRAFT_2951 0.53 +0.2 260,717 - +0.3 260,752 - +0.8 260,822 + -0.5 260,822 + -0.3 260,822 + -1.1 260,822 + -0.3 260,823 - +2.0 260,823 - +0.2 260,823 - +0.0 260,823 - -1.1 260,852 + EX28DRAFT_2952 0.12 -0.7 260,852 + EX28DRAFT_2952 0.12 +0.5 260,852 + EX28DRAFT_2952 0.12 -0.1 260,853 - EX28DRAFT_2952 0.12 +0.4 260,853 - EX28DRAFT_2952 0.12 -0.2 260,853 - EX28DRAFT_2952 0.12 -0.8 260,853 - EX28DRAFT_2952 0.12 +0.3 260,927 - EX28DRAFT_2952 0.22 -0.2 261,066 + EX28DRAFT_2952 0.41 +0.2 261,082 + EX28DRAFT_2952 0.43 +0.0 261,106 + EX28DRAFT_2952 0.47 +1.4 261,108 + EX28DRAFT_2952 0.47 -1.3 261,203 + EX28DRAFT_2952 0.60 +0.2
Or see this region's nucleotide sequence