Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2936 and EX28DRAFT_2937 are separated by 22 nucleotides EX28DRAFT_2937 and EX28DRAFT_2938 are separated by 554 nucleotides EX28DRAFT_2938 and EX28DRAFT_2939 are separated by 80 nucleotides EX28DRAFT_2939 and EX28DRAFT_2940 are separated by 163 nucleotides
EX28DRAFT_2936: EX28DRAFT_2936 - Uracil DNA glycosylase superfamily, at 247,744 to 248,346
_2936
EX28DRAFT_2937: EX28DRAFT_2937 - hypothetical protein, at 248,369 to 248,614
_2937
EX28DRAFT_2938: EX28DRAFT_2938 - tRNA, at 249,169 to 249,242
_2938
EX28DRAFT_2939: EX28DRAFT_2939 - Membrane proteins related to metalloendopeptidases, at 249,323 to 250,066
_2939
EX28DRAFT_2940: EX28DRAFT_2940 - isopentenyl-diphosphate delta-isomerase (EC 5.3.3.2), at 250,230 to 250,772
_2940
Position (kb) 249
250 Strain fitness (log2 ratio) -1
0
1
2 at 248.233 kb on + strand, within EX28DRAFT_2936 at 248.233 kb on + strand, within EX28DRAFT_2936 at 248.413 kb on - strand, within EX28DRAFT_2937 at 248.414 kb on + strand, within EX28DRAFT_2937 at 248.414 kb on + strand, within EX28DRAFT_2937 at 248.415 kb on - strand, within EX28DRAFT_2937 at 248.417 kb on - strand, within EX28DRAFT_2937 at 248.417 kb on - strand, within EX28DRAFT_2937 at 248.423 kb on + strand, within EX28DRAFT_2937 at 248.424 kb on - strand, within EX28DRAFT_2937 at 248.424 kb on - strand, within EX28DRAFT_2937 at 248.509 kb on - strand, within EX28DRAFT_2937 at 248.512 kb on + strand, within EX28DRAFT_2937 at 248.513 kb on - strand, within EX28DRAFT_2937 at 248.523 kb on + strand, within EX28DRAFT_2937 at 248.590 kb on + strand at 248.590 kb on + strand at 248.627 kb on + strand at 248.631 kb on + strand at 248.683 kb on - strand at 248.701 kb on + strand at 248.710 kb on + strand at 248.711 kb on - strand at 248.844 kb on - strand at 248.864 kb on + strand at 248.865 kb on - strand at 248.865 kb on - strand at 248.931 kb on + strand at 248.971 kb on - strand at 249.081 kb on + strand at 249.093 kb on + strand at 249.098 kb on + strand at 249.099 kb on - strand at 249.223 kb on + strand, within EX28DRAFT_2938 at 249.264 kb on + strand at 249.264 kb on + strand at 249.264 kb on + strand at 249.283 kb on + strand at 249.283 kb on + strand at 249.284 kb on - strand at 249.284 kb on - strand at 249.388 kb on - strand at 249.505 kb on - strand, within EX28DRAFT_2939 at 249.532 kb on + strand, within EX28DRAFT_2939 at 249.532 kb on + strand, within EX28DRAFT_2939 at 249.532 kb on + strand, within EX28DRAFT_2939 at 249.533 kb on - strand, within EX28DRAFT_2939 at 249.533 kb on - strand, within EX28DRAFT_2939 at 249.576 kb on + strand, within EX28DRAFT_2939 at 249.577 kb on - strand, within EX28DRAFT_2939 at 249.603 kb on + strand, within EX28DRAFT_2939 at 249.846 kb on + strand, within EX28DRAFT_2939 at 249.846 kb on + strand, within EX28DRAFT_2939 at 249.847 kb on - strand, within EX28DRAFT_2939 at 249.847 kb on - strand, within EX28DRAFT_2939 at 249.957 kb on + strand, within EX28DRAFT_2939 at 249.958 kb on - strand, within EX28DRAFT_2939 at 249.958 kb on - strand, within EX28DRAFT_2939 at 250.004 kb on + strand at 250.098 kb on + strand at 250.098 kb on + strand at 250.099 kb on - strand at 250.119 kb on + strand at 250.164 kb on - strand at 250.174 kb on + strand at 250.175 kb on - strand at 250.175 kb on - strand at 250.176 kb on + strand at 250.176 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 248,233 + EX28DRAFT_2936 0.81 +0.7 248,233 + EX28DRAFT_2936 0.81 +0.2 248,413 - EX28DRAFT_2937 0.18 +0.5 248,414 + EX28DRAFT_2937 0.18 -0.6 248,414 + EX28DRAFT_2937 0.18 -0.8 248,415 - EX28DRAFT_2937 0.19 +0.4 248,417 - EX28DRAFT_2937 0.20 +1.0 248,417 - EX28DRAFT_2937 0.20 +0.0 248,423 + EX28DRAFT_2937 0.22 -0.2 248,424 - EX28DRAFT_2937 0.22 +0.3 248,424 - EX28DRAFT_2937 0.22 +0.1 248,509 - EX28DRAFT_2937 0.57 +0.2 248,512 + EX28DRAFT_2937 0.58 -0.5 248,513 - EX28DRAFT_2937 0.59 +0.3 248,523 + EX28DRAFT_2937 0.63 +0.9 248,590 + +0.2 248,590 + -0.2 248,627 + -0.1 248,631 + -0.1 248,683 - +0.1 248,701 + +0.4 248,710 + +0.4 248,711 - +1.3 248,844 - +0.9 248,864 + -0.1 248,865 - +0.9 248,865 - -0.0 248,931 + +0.2 248,971 - -0.2 249,081 + +0.4 249,093 + +0.8 249,098 + +1.7 249,099 - +1.2 249,223 + EX28DRAFT_2938 0.73 -0.8 249,264 + +0.3 249,264 + +0.6 249,264 + +0.8 249,283 + +0.2 249,283 + -1.7 249,284 - +0.3 249,284 - +1.3 249,388 - +0.9 249,505 - EX28DRAFT_2939 0.24 -0.5 249,532 + EX28DRAFT_2939 0.28 +0.2 249,532 + EX28DRAFT_2939 0.28 -0.2 249,532 + EX28DRAFT_2939 0.28 -0.3 249,533 - EX28DRAFT_2939 0.28 -0.1 249,533 - EX28DRAFT_2939 0.28 -0.8 249,576 + EX28DRAFT_2939 0.34 +0.0 249,577 - EX28DRAFT_2939 0.34 -0.7 249,603 + EX28DRAFT_2939 0.38 -0.3 249,846 + EX28DRAFT_2939 0.70 -0.7 249,846 + EX28DRAFT_2939 0.70 +0.0 249,847 - EX28DRAFT_2939 0.70 +0.9 249,847 - EX28DRAFT_2939 0.70 +0.1 249,957 + EX28DRAFT_2939 0.85 -0.1 249,958 - EX28DRAFT_2939 0.85 +0.5 249,958 - EX28DRAFT_2939 0.85 +0.0 250,004 + +1.9 250,098 + +0.1 250,098 + +0.0 250,099 - +0.3 250,119 + +0.5 250,164 - +0.4 250,174 + -0.4 250,175 - +0.1 250,175 - -0.3 250,176 + +0.7 250,176 + -0.2
Or see this region's nucleotide sequence