Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2906 and EX28DRAFT_2907 are separated by 128 nucleotides EX28DRAFT_2907 and EX28DRAFT_2908 are separated by 131 nucleotides
EX28DRAFT_2906: EX28DRAFT_2906 - DNA mismatch repair protein MutH, at 217,436 to 218,128
_2906
EX28DRAFT_2907: EX28DRAFT_2907 - Bacterial protein of unknown function (DUF903), at 218,257 to 218,475
_2907
EX28DRAFT_2908: EX28DRAFT_2908 - Predicted oxidoreductases (related to aryl-alcohol dehydrogenases), at 218,607 to 219,647
_2908
Position (kb)
218
219 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 217.373 kb on + strand at 217.373 kb on + strand at 217.385 kb on - strand at 217.417 kb on + strand at 217.447 kb on + strand at 217.489 kb on - strand at 217.489 kb on - strand at 217.669 kb on - strand, within EX28DRAFT_2906 at 217.726 kb on - strand, within EX28DRAFT_2906 at 217.787 kb on - strand, within EX28DRAFT_2906 at 217.951 kb on + strand, within EX28DRAFT_2906 at 217.974 kb on + strand, within EX28DRAFT_2906 at 217.975 kb on - strand, within EX28DRAFT_2906 at 217.975 kb on - strand, within EX28DRAFT_2906 at 217.975 kb on - strand, within EX28DRAFT_2906 at 217.978 kb on - strand, within EX28DRAFT_2906 at 218.069 kb on + strand at 218.126 kb on + strand at 218.127 kb on - strand at 218.159 kb on + strand at 218.222 kb on + strand at 218.222 kb on + strand at 218.223 kb on - strand at 218.277 kb on - strand at 218.317 kb on - strand, within EX28DRAFT_2907 at 218.327 kb on - strand, within EX28DRAFT_2907 at 218.454 kb on - strand at 218.463 kb on + strand at 218.548 kb on + strand at 218.548 kb on + strand at 218.549 kb on - strand at 218.549 kb on - strand at 218.549 kb on - strand at 218.549 kb on - strand at 218.550 kb on + strand at 218.550 kb on + strand at 218.582 kb on + strand at 218.582 kb on + strand at 218.582 kb on + strand at 218.583 kb on - strand at 218.584 kb on + strand at 218.584 kb on + strand at 218.584 kb on + strand at 218.584 kb on + strand at 218.584 kb on + strand at 218.585 kb on - strand at 218.585 kb on - strand at 218.718 kb on + strand, within EX28DRAFT_2908 at 218.763 kb on + strand, within EX28DRAFT_2908 at 218.763 kb on + strand, within EX28DRAFT_2908 at 218.764 kb on - strand, within EX28DRAFT_2908 at 219.249 kb on + strand, within EX28DRAFT_2908 at 219.249 kb on + strand, within EX28DRAFT_2908 at 219.249 kb on + strand, within EX28DRAFT_2908 at 219.250 kb on - strand, within EX28DRAFT_2908 at 219.250 kb on - strand, within EX28DRAFT_2908 at 219.250 kb on - strand, within EX28DRAFT_2908 at 219.250 kb on - strand, within EX28DRAFT_2908 at 219.304 kb on - strand, within EX28DRAFT_2908 at 219.323 kb on + strand, within EX28DRAFT_2908 at 219.339 kb on + strand, within EX28DRAFT_2908 at 219.399 kb on + strand, within EX28DRAFT_2908 at 219.400 kb on - strand, within EX28DRAFT_2908 at 219.436 kb on - strand, within EX28DRAFT_2908
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 217,373 + +0.4 217,373 + -0.1 217,385 - +0.2 217,417 + +0.1 217,447 + -2.4 217,489 - -0.2 217,489 - -0.2 217,669 - EX28DRAFT_2906 0.34 +0.3 217,726 - EX28DRAFT_2906 0.42 +0.3 217,787 - EX28DRAFT_2906 0.51 +0.2 217,951 + EX28DRAFT_2906 0.74 +0.4 217,974 + EX28DRAFT_2906 0.78 +0.4 217,975 - EX28DRAFT_2906 0.78 +0.6 217,975 - EX28DRAFT_2906 0.78 -0.0 217,975 - EX28DRAFT_2906 0.78 +0.9 217,978 - EX28DRAFT_2906 0.78 +0.1 218,069 + -0.1 218,126 + +0.6 218,127 - +0.5 218,159 + +0.0 218,222 + +0.2 218,222 + -0.1 218,223 - +1.1 218,277 - +0.3 218,317 - EX28DRAFT_2907 0.27 -1.1 218,327 - EX28DRAFT_2907 0.32 -0.3 218,454 - -2.1 218,463 + +0.3 218,548 + +0.3 218,548 + -0.0 218,549 - +0.2 218,549 - -0.4 218,549 - +0.1 218,549 - +1.2 218,550 + +0.6 218,550 + +0.1 218,582 + -0.6 218,582 + -1.1 218,582 + -0.4 218,583 - +0.3 218,584 + -1.4 218,584 + +0.4 218,584 + -0.4 218,584 + -0.4 218,584 + +0.1 218,585 - -0.2 218,585 - -0.1 218,718 + EX28DRAFT_2908 0.11 -0.5 218,763 + EX28DRAFT_2908 0.15 +0.2 218,763 + EX28DRAFT_2908 0.15 -0.2 218,764 - EX28DRAFT_2908 0.15 -0.3 219,249 + EX28DRAFT_2908 0.62 +0.3 219,249 + EX28DRAFT_2908 0.62 +0.1 219,249 + EX28DRAFT_2908 0.62 +0.0 219,250 - EX28DRAFT_2908 0.62 +0.2 219,250 - EX28DRAFT_2908 0.62 +1.8 219,250 - EX28DRAFT_2908 0.62 -1.1 219,250 - EX28DRAFT_2908 0.62 -0.6 219,304 - EX28DRAFT_2908 0.67 -1.3 219,323 + EX28DRAFT_2908 0.69 +0.2 219,339 + EX28DRAFT_2908 0.70 +0.2 219,399 + EX28DRAFT_2908 0.76 -0.1 219,400 - EX28DRAFT_2908 0.76 -1.0 219,436 - EX28DRAFT_2908 0.80 +0.2
Or see this region's nucleotide sequence