Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2892 and EX28DRAFT_2893 are separated by 212 nucleotides EX28DRAFT_2893 and EX28DRAFT_2894 are separated by 34 nucleotides
EX28DRAFT_2892: EX28DRAFT_2892 - N-acetylmuramoyl-L-alanine amidase, at 195,589 to 196,857
_2892
EX28DRAFT_2893: EX28DRAFT_2893 - N-acetylglutamate synthase (EC 2.3.1.1), at 197,070 to 198,401
_2893
EX28DRAFT_2894: EX28DRAFT_2894 - DNA helicase/exodeoxyribonuclease V, alpha subunit (EC 3.1.11.5), at 198,436 to 200,256
_2894
Position (kb) 197
198
199 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 196.119 kb on + strand, within EX28DRAFT_2892 at 196.137 kb on + strand, within EX28DRAFT_2892 at 196.137 kb on + strand, within EX28DRAFT_2892 at 196.140 kb on - strand, within EX28DRAFT_2892 at 196.140 kb on - strand, within EX28DRAFT_2892 at 196.149 kb on + strand, within EX28DRAFT_2892 at 196.361 kb on + strand, within EX28DRAFT_2892 at 196.395 kb on + strand, within EX28DRAFT_2892 at 196.440 kb on + strand, within EX28DRAFT_2892 at 196.441 kb on - strand, within EX28DRAFT_2892 at 196.664 kb on + strand, within EX28DRAFT_2892 at 196.665 kb on - strand, within EX28DRAFT_2892 at 196.704 kb on - strand, within EX28DRAFT_2892 at 196.704 kb on - strand, within EX28DRAFT_2892 at 196.801 kb on + strand at 196.874 kb on - strand at 196.928 kb on + strand at 196.929 kb on - strand at 196.929 kb on - strand at 196.949 kb on - strand at 197.084 kb on + strand at 197.085 kb on - strand at 197.085 kb on - strand at 197.085 kb on - strand at 197.125 kb on - strand at 197.256 kb on + strand, within EX28DRAFT_2893 at 197.256 kb on + strand, within EX28DRAFT_2893 at 197.257 kb on - strand, within EX28DRAFT_2893 at 197.257 kb on - strand, within EX28DRAFT_2893 at 197.423 kb on + strand, within EX28DRAFT_2893 at 197.423 kb on + strand, within EX28DRAFT_2893 at 197.549 kb on + strand, within EX28DRAFT_2893 at 197.549 kb on + strand, within EX28DRAFT_2893 at 197.550 kb on - strand, within EX28DRAFT_2893 at 197.877 kb on + strand, within EX28DRAFT_2893 at 198.189 kb on - strand, within EX28DRAFT_2893 at 198.354 kb on + strand at 198.354 kb on + strand at 198.354 kb on + strand at 198.355 kb on - strand at 198.355 kb on - strand at 198.355 kb on - strand at 198.360 kb on + strand at 198.360 kb on + strand at 198.361 kb on - strand at 198.361 kb on - strand at 198.361 kb on - strand at 198.560 kb on + strand at 198.560 kb on + strand at 198.560 kb on + strand at 198.561 kb on - strand at 198.580 kb on + strand at 198.580 kb on + strand at 198.580 kb on + strand at 198.580 kb on + strand at 198.642 kb on + strand, within EX28DRAFT_2894 at 198.643 kb on - strand, within EX28DRAFT_2894 at 198.948 kb on - strand, within EX28DRAFT_2894 at 198.982 kb on - strand, within EX28DRAFT_2894 at 198.995 kb on - strand, within EX28DRAFT_2894 at 198.995 kb on - strand, within EX28DRAFT_2894 at 199.049 kb on + strand, within EX28DRAFT_2894 at 199.084 kb on - strand, within EX28DRAFT_2894 at 199.313 kb on + strand, within EX28DRAFT_2894
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 196,119 + EX28DRAFT_2892 0.42 -0.3 196,137 + EX28DRAFT_2892 0.43 +0.2 196,137 + EX28DRAFT_2892 0.43 +0.2 196,140 - EX28DRAFT_2892 0.43 -0.1 196,140 - EX28DRAFT_2892 0.43 -0.6 196,149 + EX28DRAFT_2892 0.44 -0.0 196,361 + EX28DRAFT_2892 0.61 +0.0 196,395 + EX28DRAFT_2892 0.64 +0.2 196,440 + EX28DRAFT_2892 0.67 -0.7 196,441 - EX28DRAFT_2892 0.67 +0.4 196,664 + EX28DRAFT_2892 0.85 -0.1 196,665 - EX28DRAFT_2892 0.85 +0.3 196,704 - EX28DRAFT_2892 0.88 +0.1 196,704 - EX28DRAFT_2892 0.88 +0.1 196,801 + -1.9 196,874 - +1.1 196,928 + +0.2 196,929 - +1.0 196,929 - +0.3 196,949 - +0.3 197,084 + -2.6 197,085 - -1.2 197,085 - -4.6 197,085 - -1.4 197,125 - -4.2 197,256 + EX28DRAFT_2893 0.14 -4.8 197,256 + EX28DRAFT_2893 0.14 -3.5 197,257 - EX28DRAFT_2893 0.14 -2.4 197,257 - EX28DRAFT_2893 0.14 -3.3 197,423 + EX28DRAFT_2893 0.27 -3.0 197,423 + EX28DRAFT_2893 0.27 -1.7 197,549 + EX28DRAFT_2893 0.36 -3.5 197,549 + EX28DRAFT_2893 0.36 -3.0 197,550 - EX28DRAFT_2893 0.36 -5.2 197,877 + EX28DRAFT_2893 0.61 -3.6 198,189 - EX28DRAFT_2893 0.84 -0.8 198,354 + -2.7 198,354 + -5.6 198,354 + -2.5 198,355 - -5.0 198,355 - -4.6 198,355 - -3.3 198,360 + -3.1 198,360 + -4.1 198,361 - -0.4 198,361 - -1.3 198,361 - -4.1 198,560 + +2.0 198,560 + -0.7 198,560 + -0.3 198,561 - +0.3 198,580 + -0.2 198,580 + +0.3 198,580 + -0.6 198,580 + -0.1 198,642 + EX28DRAFT_2894 0.11 +0.9 198,643 - EX28DRAFT_2894 0.11 -0.2 198,948 - EX28DRAFT_2894 0.28 +1.0 198,982 - EX28DRAFT_2894 0.30 -1.1 198,995 - EX28DRAFT_2894 0.31 +0.8 198,995 - EX28DRAFT_2894 0.31 +0.7 199,049 + EX28DRAFT_2894 0.34 +0.5 199,084 - EX28DRAFT_2894 0.36 -0.2 199,313 + EX28DRAFT_2894 0.48 -0.3
Or see this region's nucleotide sequence