Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2886 and EX28DRAFT_2887 overlap by 1 nucleotides EX28DRAFT_2887 and EX28DRAFT_2888 overlap by 10 nucleotides EX28DRAFT_2888 and EX28DRAFT_2889 are separated by 75 nucleotides
EX28DRAFT_2886: EX28DRAFT_2886 - cysteine desulfurase, catalytic subunit CsdA, at 191,473 to 192,678
_2886
EX28DRAFT_2887: EX28DRAFT_2887 - cysteine desulfurase, sulfur acceptor subunit CsdE, at 192,678 to 193,124
_2887
EX28DRAFT_2888: EX28DRAFT_2888 - Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 1, at 193,115 to 193,921
_2888
EX28DRAFT_2889: EX28DRAFT_2889 - Membrane-bound lytic murein transglycosylase, at 193,997 to 195,094
_2889
Position (kb) 193
194 Strain fitness (log2 ratio) -1
0
1
2 at 192.119 kb on - strand, within EX28DRAFT_2886 at 192.135 kb on + strand, within EX28DRAFT_2886 at 192.135 kb on + strand, within EX28DRAFT_2886 at 192.135 kb on + strand, within EX28DRAFT_2886 at 192.136 kb on - strand, within EX28DRAFT_2886 at 192.136 kb on - strand, within EX28DRAFT_2886 at 192.610 kb on - strand at 192.612 kb on + strand at 192.665 kb on + strand at 192.666 kb on - strand at 192.684 kb on - strand at 192.737 kb on - strand, within EX28DRAFT_2887 at 192.773 kb on + strand, within EX28DRAFT_2887 at 192.774 kb on - strand, within EX28DRAFT_2887 at 192.777 kb on + strand, within EX28DRAFT_2887 at 192.850 kb on + strand, within EX28DRAFT_2887 at 192.851 kb on - strand, within EX28DRAFT_2887 at 192.851 kb on - strand, within EX28DRAFT_2887 at 193.551 kb on + strand, within EX28DRAFT_2888 at 193.551 kb on + strand, within EX28DRAFT_2888 at 193.552 kb on - strand, within EX28DRAFT_2888 at 193.557 kb on + strand, within EX28DRAFT_2888 at 193.558 kb on - strand, within EX28DRAFT_2888 at 193.558 kb on - strand, within EX28DRAFT_2888 at 193.574 kb on - strand, within EX28DRAFT_2888 at 193.751 kb on - strand, within EX28DRAFT_2888 at 193.865 kb on + strand at 193.866 kb on - strand at 193.922 kb on - strand at 193.922 kb on - strand at 193.935 kb on + strand at 193.935 kb on + strand at 193.935 kb on + strand at 193.936 kb on - strand at 193.936 kb on - strand at 193.968 kb on + strand at 193.969 kb on - strand at 193.969 kb on - strand at 194.064 kb on + strand at 194.064 kb on + strand at 194.065 kb on - strand at 194.098 kb on + strand at 194.106 kb on + strand at 194.106 kb on + strand at 194.107 kb on - strand, within EX28DRAFT_2889 at 194.107 kb on - strand, within EX28DRAFT_2889 at 194.194 kb on + strand, within EX28DRAFT_2889 at 194.261 kb on + strand, within EX28DRAFT_2889 at 194.496 kb on + strand, within EX28DRAFT_2889 at 194.496 kb on + strand, within EX28DRAFT_2889 at 194.497 kb on - strand, within EX28DRAFT_2889 at 194.538 kb on + strand, within EX28DRAFT_2889 at 194.589 kb on + strand, within EX28DRAFT_2889 at 194.602 kb on - strand, within EX28DRAFT_2889 at 194.668 kb on + strand, within EX28DRAFT_2889 at 194.673 kb on + strand, within EX28DRAFT_2889 at 194.674 kb on - strand, within EX28DRAFT_2889 at 194.677 kb on + strand, within EX28DRAFT_2889 at 194.678 kb on - strand, within EX28DRAFT_2889 at 194.730 kb on + strand, within EX28DRAFT_2889 at 194.730 kb on + strand, within EX28DRAFT_2889 at 194.806 kb on + strand, within EX28DRAFT_2889 at 194.806 kb on + strand, within EX28DRAFT_2889 at 194.853 kb on + strand, within EX28DRAFT_2889 at 194.853 kb on + strand, within EX28DRAFT_2889 at 194.854 kb on - strand, within EX28DRAFT_2889 at 194.854 kb on - strand, within EX28DRAFT_2889 at 194.860 kb on + strand, within EX28DRAFT_2889 at 194.874 kb on + strand, within EX28DRAFT_2889
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 192,119 - EX28DRAFT_2886 0.54 +0.0 192,135 + EX28DRAFT_2886 0.55 -0.2 192,135 + EX28DRAFT_2886 0.55 -0.8 192,135 + EX28DRAFT_2886 0.55 -0.0 192,136 - EX28DRAFT_2886 0.55 +0.4 192,136 - EX28DRAFT_2886 0.55 +0.1 192,610 - +0.2 192,612 + +0.4 192,665 + -0.6 192,666 - -1.2 192,684 - -0.1 192,737 - EX28DRAFT_2887 0.13 -0.1 192,773 + EX28DRAFT_2887 0.21 -0.7 192,774 - EX28DRAFT_2887 0.21 +0.1 192,777 + EX28DRAFT_2887 0.22 +0.3 192,850 + EX28DRAFT_2887 0.38 +0.4 192,851 - EX28DRAFT_2887 0.39 -0.0 192,851 - EX28DRAFT_2887 0.39 +0.7 193,551 + EX28DRAFT_2888 0.54 +1.0 193,551 + EX28DRAFT_2888 0.54 -0.4 193,552 - EX28DRAFT_2888 0.54 -1.1 193,557 + EX28DRAFT_2888 0.55 +0.8 193,558 - EX28DRAFT_2888 0.55 -0.0 193,558 - EX28DRAFT_2888 0.55 +0.1 193,574 - EX28DRAFT_2888 0.57 +1.1 193,751 - EX28DRAFT_2888 0.79 +1.8 193,865 + +0.8 193,866 - -0.8 193,922 - +0.0 193,922 - +0.5 193,935 + -0.1 193,935 + -0.8 193,935 + +2.4 193,936 - -0.7 193,936 - +1.5 193,968 + -0.3 193,969 - -0.1 193,969 - +0.3 194,064 + +0.2 194,064 + +0.0 194,065 - +0.6 194,098 + -1.1 194,106 + -0.9 194,106 + -1.0 194,107 - EX28DRAFT_2889 0.10 -0.2 194,107 - EX28DRAFT_2889 0.10 +0.5 194,194 + EX28DRAFT_2889 0.18 -1.1 194,261 + EX28DRAFT_2889 0.24 +0.1 194,496 + EX28DRAFT_2889 0.45 +0.4 194,496 + EX28DRAFT_2889 0.45 +0.2 194,497 - EX28DRAFT_2889 0.46 -0.5 194,538 + EX28DRAFT_2889 0.49 -0.1 194,589 + EX28DRAFT_2889 0.54 +0.1 194,602 - EX28DRAFT_2889 0.55 +0.8 194,668 + EX28DRAFT_2889 0.61 -0.2 194,673 + EX28DRAFT_2889 0.62 +0.9 194,674 - EX28DRAFT_2889 0.62 -0.4 194,677 + EX28DRAFT_2889 0.62 +0.2 194,678 - EX28DRAFT_2889 0.62 +0.4 194,730 + EX28DRAFT_2889 0.67 +1.0 194,730 + EX28DRAFT_2889 0.67 +0.3 194,806 + EX28DRAFT_2889 0.74 -0.8 194,806 + EX28DRAFT_2889 0.74 +1.3 194,853 + EX28DRAFT_2889 0.78 -1.0 194,853 + EX28DRAFT_2889 0.78 -0.4 194,854 - EX28DRAFT_2889 0.78 +0.2 194,854 - EX28DRAFT_2889 0.78 +0.1 194,860 + EX28DRAFT_2889 0.79 -1.5 194,874 + EX28DRAFT_2889 0.80 +0.5
Or see this region's nucleotide sequence